灌溉温度和绞刀速度对骨软骨移植软骨细胞活力的影响。

Andrew R Phillips,Erik C Haneberg,Thomas E Moran,Ron Gilat,Kofi K Acheampong,Vince Morgan,Adam B Yanke
{"title":"灌溉温度和绞刀速度对骨软骨移植软骨细胞活力的影响。","authors":"Andrew R Phillips,Erik C Haneberg,Thomas E Moran,Ron Gilat,Kofi K Acheampong,Vince Morgan,Adam B Yanke","doi":"10.1177/03635465251336363","DOIUrl":null,"url":null,"abstract":"BACKGROUND\r\nPrevious literature shows that current osteochondral allograft (OCA) harvesting techniques lead to donor plug chondrocyte death during OCA transplant for treating focal knee cartilage defects.\r\n\r\nPURPOSE\r\nTo evaluate the effects of drilling/reaming speed and irrigation temperature on chondrocyte viability during OCA harvest.\r\n\r\nSTUDY DESIGN\r\nControlled laboratory study.\r\n\r\nMETHODS\r\nFrom each human femoral condyle (n = 9) used in the study, cartilage plugs were harvested using a powered reamer with 15 mm-diameter coring bit and continuous saline irrigation. Four experimental groups were formed according to the following parameters that were used to randomly harvest the plugs: (1) drill speed setting with room temperature (RT) saline; (2) ream speed setting with RT saline; (3) drill speed setting with cold saline; (4) ream speed setting with cold saline. A control cartilage sample was harvested with a scalpel from a macroscopically healthy region near the intercondylar notch in each specimen. Chondrocyte viability, determined by calcein and ethidium live/dead stain, was compared between groups and controls across the whole, central third, and peripheral thirds of cartilage samples.\r\n\r\nRESULTS\r\nWhole drill RT (P = .001) and whole ream RT (P = .004) viabilities were significantly decreased in experimental samples compared with controls. Viability at the center and periphery of drill RT plugs (P = .001 and P < .001, respectively) and ream RT plugs (P = .001 and P = .002, respectively) were also decreased. Conversely, viability was not significantly different between controls and either whole drill cold (P = .17), center drill cold (P = .24), periphery drill cold (P = .20), whole ream cold (P = .15), center ream cold (P = .24), or periphery ream cold (P = .07) samples. Viability was decreased in whole drill RT compared with whole ream RT (P = .015), but there was no difference between whole drill cold and whole ream cold (P = .26).\r\n\r\nCONCLUSION\r\nContinuous irrigation with 5°C saline rather than RT saline during OCA harvest maintained donor graft chondrocyte viability in vitro. With RT irrigation, faster reamer speed induced more chondrocyte death than slower reamer speed, although this difference was mitigated with 5°C saline irrigation.\r\n\r\nCLINICAL RELEVANCE\r\nIrrigation with 5°C saline during donor plug OCA harvest may mitigate thermally induced chondrocyte damage and improve graft incorporation, healing, and outcomes of OCA transplant.","PeriodicalId":517411,"journal":{"name":"The American Journal of Sports Medicine","volume":"111 1","pages":"3635465251336363"},"PeriodicalIF":0.0000,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Osteochondral Allograft Chondrocyte Viability Is Affected During Harvest by Irrigation Temperature and Reamer Speed.\",\"authors\":\"Andrew R Phillips,Erik C Haneberg,Thomas E Moran,Ron Gilat,Kofi K Acheampong,Vince Morgan,Adam B Yanke\",\"doi\":\"10.1177/03635465251336363\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"BACKGROUND\\r\\nPrevious literature shows that current osteochondral allograft (OCA) harvesting techniques lead to donor plug chondrocyte death during OCA transplant for treating focal knee cartilage defects.\\r\\n\\r\\nPURPOSE\\r\\nTo evaluate the effects of drilling/reaming speed and irrigation temperature on chondrocyte viability during OCA harvest.\\r\\n\\r\\nSTUDY DESIGN\\r\\nControlled laboratory study.\\r\\n\\r\\nMETHODS\\r\\nFrom each human femoral condyle (n = 9) used in the study, cartilage plugs were harvested using a powered reamer with 15 mm-diameter coring bit and continuous saline irrigation. Four experimental groups were formed according to the following parameters that were used to randomly harvest the plugs: (1) drill speed setting with room temperature (RT) saline; (2) ream speed setting with RT saline; (3) drill speed setting with cold saline; (4) ream speed setting with cold saline. A control cartilage sample was harvested with a scalpel from a macroscopically healthy region near the intercondylar notch in each specimen. Chondrocyte viability, determined by calcein and ethidium live/dead stain, was compared between groups and controls across the whole, central third, and peripheral thirds of cartilage samples.\\r\\n\\r\\nRESULTS\\r\\nWhole drill RT (P = .001) and whole ream RT (P = .004) viabilities were significantly decreased in experimental samples compared with controls. Viability at the center and periphery of drill RT plugs (P = .001 and P < .001, respectively) and ream RT plugs (P = .001 and P = .002, respectively) were also decreased. Conversely, viability was not significantly different between controls and either whole drill cold (P = .17), center drill cold (P = .24), periphery drill cold (P = .20), whole ream cold (P = .15), center ream cold (P = .24), or periphery ream cold (P = .07) samples. Viability was decreased in whole drill RT compared with whole ream RT (P = .015), but there was no difference between whole drill cold and whole ream cold (P = .26).\\r\\n\\r\\nCONCLUSION\\r\\nContinuous irrigation with 5°C saline rather than RT saline during OCA harvest maintained donor graft chondrocyte viability in vitro. With RT irrigation, faster reamer speed induced more chondrocyte death than slower reamer speed, although this difference was mitigated with 5°C saline irrigation.\\r\\n\\r\\nCLINICAL RELEVANCE\\r\\nIrrigation with 5°C saline during donor plug OCA harvest may mitigate thermally induced chondrocyte damage and improve graft incorporation, healing, and outcomes of OCA transplant.\",\"PeriodicalId\":517411,\"journal\":{\"name\":\"The American Journal of Sports Medicine\",\"volume\":\"111 1\",\"pages\":\"3635465251336363\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2025-05-12\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"The American Journal of Sports Medicine\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1177/03635465251336363\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"The American Journal of Sports Medicine","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1177/03635465251336363","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

摘要

背景:先前的文献表明,目前的同种异体骨软骨移植(OCA)技术在治疗局灶性膝关节软骨缺损的OCA移植过程中会导致供体塞软骨细胞死亡。目的探讨钻孔/扩孔速度和灌洗温度对OCA收获过程中软骨细胞活力的影响。研究设计:对照实验室研究。方法从研究中使用的每个人股骨髁(n = 9)中,使用直径为15mm取心钻头的动力铰刀和连续盐水冲洗获取软骨塞。根据以下参数组成4个实验组,随机取钻塞:(1)室温生理盐水设定钻速;(2) RT生理盐水调眼速度;(3)用冷盐水设定钻速;(4)用冷生理盐水设定粒速。对照软骨样本用手术刀从每个标本中靠近髁间切迹的宏观健康区域采集。通过钙黄蛋白和乙硫铵活/死染色测定软骨细胞活力,比较各组和对照组在整个、中心三分之一和外周三分之一软骨样本中的活力。结果与对照组相比,试验组全钻RT (P = 0.001)和全团RT (P = 0.004)存活率均显著降低。钻孔RT塞(P = 0.001和P < 0.001)和钻孔RT塞(P = 0.001和P = 0.002)中心和周围的活力也下降。相反,对照组与全钻冷(P = 0.17)、中心钻冷(P = 0.24)、外围钻冷(P = 0.20)、全钻冷(P = 0.15)、中心钻冷(P = 0.24)或外围钻冷(P = 0.07)样品之间的活力差异不显著。全钻低温处理与全圈低温处理相比,存活率降低(P = 0.015),但全钻低温处理与全圈低温处理之间差异无统计学意义(P = 0.26)。结论在OCA收获过程中,持续用5°C生理盐水而非RT生理盐水冲洗可维持供体软骨细胞的体外活力。使用RT冲洗时,更快的扩眼器速度比更慢的扩眼器速度诱导更多的软骨细胞死亡,尽管5°C盐水冲洗可以减轻这种差异。临床意义:供体塞OCA收获期间用5°C生理盐水冲洗可以减轻热诱导的软骨细胞损伤,改善移植物融合、愈合和OCA移植的结果。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Osteochondral Allograft Chondrocyte Viability Is Affected During Harvest by Irrigation Temperature and Reamer Speed.
BACKGROUND Previous literature shows that current osteochondral allograft (OCA) harvesting techniques lead to donor plug chondrocyte death during OCA transplant for treating focal knee cartilage defects. PURPOSE To evaluate the effects of drilling/reaming speed and irrigation temperature on chondrocyte viability during OCA harvest. STUDY DESIGN Controlled laboratory study. METHODS From each human femoral condyle (n = 9) used in the study, cartilage plugs were harvested using a powered reamer with 15 mm-diameter coring bit and continuous saline irrigation. Four experimental groups were formed according to the following parameters that were used to randomly harvest the plugs: (1) drill speed setting with room temperature (RT) saline; (2) ream speed setting with RT saline; (3) drill speed setting with cold saline; (4) ream speed setting with cold saline. A control cartilage sample was harvested with a scalpel from a macroscopically healthy region near the intercondylar notch in each specimen. Chondrocyte viability, determined by calcein and ethidium live/dead stain, was compared between groups and controls across the whole, central third, and peripheral thirds of cartilage samples. RESULTS Whole drill RT (P = .001) and whole ream RT (P = .004) viabilities were significantly decreased in experimental samples compared with controls. Viability at the center and periphery of drill RT plugs (P = .001 and P < .001, respectively) and ream RT plugs (P = .001 and P = .002, respectively) were also decreased. Conversely, viability was not significantly different between controls and either whole drill cold (P = .17), center drill cold (P = .24), periphery drill cold (P = .20), whole ream cold (P = .15), center ream cold (P = .24), or periphery ream cold (P = .07) samples. Viability was decreased in whole drill RT compared with whole ream RT (P = .015), but there was no difference between whole drill cold and whole ream cold (P = .26). CONCLUSION Continuous irrigation with 5°C saline rather than RT saline during OCA harvest maintained donor graft chondrocyte viability in vitro. With RT irrigation, faster reamer speed induced more chondrocyte death than slower reamer speed, although this difference was mitigated with 5°C saline irrigation. CLINICAL RELEVANCE Irrigation with 5°C saline during donor plug OCA harvest may mitigate thermally induced chondrocyte damage and improve graft incorporation, healing, and outcomes of OCA transplant.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信