Vincristine Loaded Bioinspired Vesicles: Preparation, Characterization and In-Vitro Evaluation on U87-MG Cells

Proposed work is focused on isolation of nanovesicles from Catharanthus roseus flower and Vincristine loading in it and exploration of biological activity in U87-MG and C6 cells. Nanovesicles (CDNs) were isolated by differential centrifugation followed by ultracentrifugation. Tween 80 employed as stabilizer to reduce agglomeration of particles. Spherical morphology and lipid bilayer confirmation by HRTEM. The size, PDI, and zeta potential of stable CDNs were ~ 80 nm, 0.2, and − 20 mV, respectively. The DL and EE were 12.4% and 84.6%, respectively. FTIR and DSC studies confirm encapsulation of Vincristine within CDNs. In vitro release of Vincristine from CDNs was found to follow Higuchi diffusion pattern. Drug-loaded CDNs depicted prominent cytotoxicity activity in U87 and C6 cells with ~ two times in comparison to plain CDNs and free drugs. No significant cytotoxicity was observed in HEK 293 T cells. Drug-loaded CDNs depicted prominent cellular uptake in U87-MG cells after 12 h of incubation. CDNs exhibited promising antioxidant activity when studied in ROS-induced cells as well as through DPPH and FRAP assay. In conclusion, this study, for the first time, proposed the use of CDNs as drug carriers and loading of Vincristine within exosomes isolated from its source only with prominent anti-glioma activity. This duo-drug carrier combination can be a promising approach for glioma patients after prior preclinical evaluation.