Simulated Dump Yard Microbes Drive Significant Biodegradation of Polypropylene and Polyvinyl Chloride Microplastics

Bacterial culture isolates were used to demonstrate the breakdown of polypropylene (PPs) and polyvinyl chloride (PVCs) microplastics for the first time. Using metagenomics and phylogenetic analysis, bacterial isolates were discovered from a simulated dump yard. PPs and PVCs were broken down using bacterial isolates found to be Acinetobacter baumannii and mixed culture species with a Bacillus sp. dominance. After 50 days of activity, the dry weight of microplastics decreased by 33.3% (PPs) and 27.1% (PVCs) due to the breakdown process aided by Acinetobacter baumannii.Weight reductions of 20.3% for PPs and 18.2% for PVCs have been observed in mixed bacterial cultures, respectively. Microplastics' thermal stability and transition properties changed, according to experiments like DTG, NMR, Raman, and WCA. FTIR analysis captured the structural changes in PPs and PVCs. SEM, TEM, and cell hydrophobicity tests showed that microplastics were biodegrading. GC-MS identified the released byproducts, which included carboxylic acids, alkanes, esters, and aromatic chemicals. Based on these findings, it can be concluded that bacterial isolates are effective in degrading PPs and PVCs and may help create sustainable methods of handling plastic trash.