Teguh Santoso, Astrid Feinisa Khairani, Muhammad Hasan Bashari, Julia Ramadhanti, Enny Rohmawati, Monika Pury Oktora, Hesti Lina Wiraswati
{"title":"广藿香乙醇提取物广藿香阿霉素诱导的3T3-L1成纤维细胞抗衰老谱","authors":"Teguh Santoso, Astrid Feinisa Khairani, Muhammad Hasan Bashari, Julia Ramadhanti, Enny Rohmawati, Monika Pury Oktora, Hesti Lina Wiraswati","doi":"10.1002/agm2.70014","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Objectives</h3>\n \n <p>This research aimed to investigate the potential of \n <i>Pogostemon cablin</i>\n Benth's ethanolic extract (PCEE) as an antiaging agent.</p>\n </section>\n \n <section>\n \n <h3> Methods</h3>\n \n <p>We treated 3T3-L1 cells induced by doxorubicin with PCEE and assessed them with antiaging markers such as <i>GLB-1</i> gene and telomere length with polymerase chain reaction (PCR), or reactive oxygen species (ROS) accumulation with 2,7-dichlorofluorescein diacetate (DCFHDA).</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>This study demonstrates that PCEE can inhibit ROS accumulation, decrease the expression level of the senescence-associated β-galactosidase (SA-β-gal) <i>gene, GLB-1,</i> and maintain telomere length in doxorubicin-induced 3T3-L1 cells.</p>\n </section>\n \n <section>\n \n <h3> Conclusion</h3>\n \n <p>These studies reveal that PCEE is a promising antiaging agent. An in vivo approach should be conducted for further research.</p>\n </section>\n </div>","PeriodicalId":32862,"journal":{"name":"Aging Medicine","volume":"8 2","pages":"156-163"},"PeriodicalIF":2.2000,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/agm2.70014","citationCount":"0","resultStr":"{\"title\":\"Patchouli Ethanol Extract Pogostemon cablin Benth. Against Aging Profiles in Doxorubicin-Induced 3T3-L1 Fibroblast Cell Lines\",\"authors\":\"Teguh Santoso, Astrid Feinisa Khairani, Muhammad Hasan Bashari, Julia Ramadhanti, Enny Rohmawati, Monika Pury Oktora, Hesti Lina Wiraswati\",\"doi\":\"10.1002/agm2.70014\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n \\n <section>\\n \\n <h3> Objectives</h3>\\n \\n <p>This research aimed to investigate the potential of \\n <i>Pogostemon cablin</i>\\n Benth's ethanolic extract (PCEE) as an antiaging agent.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Methods</h3>\\n \\n <p>We treated 3T3-L1 cells induced by doxorubicin with PCEE and assessed them with antiaging markers such as <i>GLB-1</i> gene and telomere length with polymerase chain reaction (PCR), or reactive oxygen species (ROS) accumulation with 2,7-dichlorofluorescein diacetate (DCFHDA).</p>\\n </section>\\n \\n <section>\\n \\n <h3> Results</h3>\\n \\n <p>This study demonstrates that PCEE can inhibit ROS accumulation, decrease the expression level of the senescence-associated β-galactosidase (SA-β-gal) <i>gene, GLB-1,</i> and maintain telomere length in doxorubicin-induced 3T3-L1 cells.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Conclusion</h3>\\n \\n <p>These studies reveal that PCEE is a promising antiaging agent. An in vivo approach should be conducted for further research.</p>\\n </section>\\n </div>\",\"PeriodicalId\":32862,\"journal\":{\"name\":\"Aging Medicine\",\"volume\":\"8 2\",\"pages\":\"156-163\"},\"PeriodicalIF\":2.2000,\"publicationDate\":\"2025-04-18\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://onlinelibrary.wiley.com/doi/epdf/10.1002/agm2.70014\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Aging Medicine\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1002/agm2.70014\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"GERIATRICS & GERONTOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Aging Medicine","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/agm2.70014","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"GERIATRICS & GERONTOLOGY","Score":null,"Total":0}
Patchouli Ethanol Extract Pogostemon cablin Benth. Against Aging Profiles in Doxorubicin-Induced 3T3-L1 Fibroblast Cell Lines
Objectives
This research aimed to investigate the potential of
Pogostemon cablin
Benth's ethanolic extract (PCEE) as an antiaging agent.
Methods
We treated 3T3-L1 cells induced by doxorubicin with PCEE and assessed them with antiaging markers such as GLB-1 gene and telomere length with polymerase chain reaction (PCR), or reactive oxygen species (ROS) accumulation with 2,7-dichlorofluorescein diacetate (DCFHDA).
Results
This study demonstrates that PCEE can inhibit ROS accumulation, decrease the expression level of the senescence-associated β-galactosidase (SA-β-gal) gene, GLB-1, and maintain telomere length in doxorubicin-induced 3T3-L1 cells.
Conclusion
These studies reveal that PCEE is a promising antiaging agent. An in vivo approach should be conducted for further research.
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