{"title":"22号针内镜超声引导下组织采集样本和玻片制备的充分性评估，以成功进行全面的基因组分析测试：单个研究所的经验","authors":"Tami Nagatani, Yoji Wani, Masahiro Takatani, Soichiro Fushimi, Hirofumi Inoue, Shinichiro Hori, Kyohei Kai, Hideki Yamamoto, Tetsuya Okazaki, Maki Tanioka, Hiroyuki Okada, Akira Hirasawa","doi":"10.1002/deo2.70104","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Objectives</h3>\n \n <p>This study aimed to evaluate the successful sequencing rate of Foundation One CDx (F1CDx) using small tissue samples obtained with a 22-gauge needle (22G) through endoscopic ultrasound-guided fine needle acquisition (EUS-TA) and to propose guidelines for tissue quantity evaluation criteria and proper slide preparation in clinical practice.</p>\n </section>\n \n <section>\n \n <h3> Methods</h3>\n \n <p>Between June 2019 and April 2024, 119 samples of 22G EUS-TA collected for F1CDx testing at Himeji Red Cross Hospital were retrospectively reviewed. Tissue adequacy was only assessed based on tumor cell percentage (≥20%). The procedure stopped when white tissue fragments reached 20 mm during macroscopic on-site evaluation. The specimens were prepared using both ‘tissue preserving sectioning’ to retain tissue within formalin-fixed paraffin-embedded blocks and the ‘thin sectioning matched needle gauge and tissue length’ method with calculation to ensure minimal unstained slides for the 1 mm<sup>3</sup> sample volume criterion. Tissue area from HE slides and sample volume were measured, and F1CDx reports were analyzed.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>Of 119 samples, 108 (90.8%) were suitable for F1CDx. Excluding the cases not submitted for testing, in the 45 cases where F1CDx was done using 22G EUS-TA samples, eight (17.8%) had a sum of tissue area tissue of 25 mm<sup>2</sup> or greater in the HE-stained sample. However, all cases met the F1CDx 1 mm<sup>3</sup> volume criterion by submitting > 30 unstained slides per sample. As a result, 43 of 45 cases (95.6%) were successfully analyzable.</p>\n </section>\n \n <section>\n \n <h3> Conclusions</h3>\n \n <p>The 22G EUS-TA needle is an effective tool for providing the sufficient tissue volume required for F1CDx.</p>\n </section>\n </div>","PeriodicalId":93973,"journal":{"name":"DEN open","volume":"6 1","pages":""},"PeriodicalIF":1.5000,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/deo2.70104","citationCount":"0","resultStr":"{\"title\":\"Adequacy evaluation of 22-gauge needle endoscopic ultrasound-guided tissue acquisition samples and glass slides preparation for successful comprehensive genomic profiling testing: A single institute experience\",\"authors\":\"Tami Nagatani, Yoji Wani, Masahiro Takatani, Soichiro Fushimi, Hirofumi Inoue, Shinichiro Hori, Kyohei Kai, Hideki Yamamoto, Tetsuya Okazaki, Maki Tanioka, Hiroyuki Okada, Akira Hirasawa\",\"doi\":\"10.1002/deo2.70104\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n \\n <section>\\n \\n <h3> Objectives</h3>\\n \\n <p>This study aimed to evaluate the successful sequencing rate of Foundation One CDx (F1CDx) using small tissue samples obtained with a 22-gauge needle (22G) through endoscopic ultrasound-guided fine needle acquisition (EUS-TA) and to propose guidelines for tissue quantity evaluation criteria and proper slide preparation in clinical practice.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Methods</h3>\\n \\n <p>Between June 2019 and April 2024, 119 samples of 22G EUS-TA collected for F1CDx testing at Himeji Red Cross Hospital were retrospectively reviewed. Tissue adequacy was only assessed based on tumor cell percentage (≥20%). The procedure stopped when white tissue fragments reached 20 mm during macroscopic on-site evaluation. The specimens were prepared using both ‘tissue preserving sectioning’ to retain tissue within formalin-fixed paraffin-embedded blocks and the ‘thin sectioning matched needle gauge and tissue length’ method with calculation to ensure minimal unstained slides for the 1 mm<sup>3</sup> sample volume criterion. Tissue area from HE slides and sample volume were measured, and F1CDx reports were analyzed.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Results</h3>\\n \\n <p>Of 119 samples, 108 (90.8%) were suitable for F1CDx. Excluding the cases not submitted for testing, in the 45 cases where F1CDx was done using 22G EUS-TA samples, eight (17.8%) had a sum of tissue area tissue of 25 mm<sup>2</sup> or greater in the HE-stained sample. 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引用次数: 0
摘要
目的本研究旨在通过内镜超声引导细针采集（EUS-TA），利用22号针（22G）获得的小组织样本，评估Foundation One CDx （F1CDx）的测序成功率，为临床实践中组织数量评估标准和正确的载玻片制备提供指导。方法回顾性分析2019年6月至2024年4月在姬路红十字会医院采集的用于F1CDx检测的22G EUS-TA样本119份。仅根据肿瘤细胞百分比（≥20%）评估组织充分性。在肉眼现场评估中，当白色组织碎片达到20毫米时，手术停止。标本的制备采用“组织保存切片”（将组织保留在福尔马林固定石蜡包埋块中）和“薄切片匹配针距和组织长度”方法，并计算以确保1 mm3样品体积标准的最小未染色载玻片。测量HE切片的组织面积和样品体积，并分析F1CDx报告。结果119份样品中有108份（90.8%）适合F1CDx检测。排除未提交检测的病例，在45例使用22G EUS-TA样本进行F1CDx的病例中，8例（17.8%）he染色样本的组织面积总和为25 mm2或更大。然而，所有病例均通过提交>；每个样品30张未染色玻片。结果45例中43例（95.6%）分析成功。结论22G EUS-TA针是提供F1CDx所需足够组织体积的有效工具。
Adequacy evaluation of 22-gauge needle endoscopic ultrasound-guided tissue acquisition samples and glass slides preparation for successful comprehensive genomic profiling testing: A single institute experience
Objectives
This study aimed to evaluate the successful sequencing rate of Foundation One CDx (F1CDx) using small tissue samples obtained with a 22-gauge needle (22G) through endoscopic ultrasound-guided fine needle acquisition (EUS-TA) and to propose guidelines for tissue quantity evaluation criteria and proper slide preparation in clinical practice.
Methods
Between June 2019 and April 2024, 119 samples of 22G EUS-TA collected for F1CDx testing at Himeji Red Cross Hospital were retrospectively reviewed. Tissue adequacy was only assessed based on tumor cell percentage (≥20%). The procedure stopped when white tissue fragments reached 20 mm during macroscopic on-site evaluation. The specimens were prepared using both ‘tissue preserving sectioning’ to retain tissue within formalin-fixed paraffin-embedded blocks and the ‘thin sectioning matched needle gauge and tissue length’ method with calculation to ensure minimal unstained slides for the 1 mm3 sample volume criterion. Tissue area from HE slides and sample volume were measured, and F1CDx reports were analyzed.
Results
Of 119 samples, 108 (90.8%) were suitable for F1CDx. Excluding the cases not submitted for testing, in the 45 cases where F1CDx was done using 22G EUS-TA samples, eight (17.8%) had a sum of tissue area tissue of 25 mm2 or greater in the HE-stained sample. However, all cases met the F1CDx 1 mm3 volume criterion by submitting > 30 unstained slides per sample. As a result, 43 of 45 cases (95.6%) were successfully analyzable.
Conclusions
The 22G EUS-TA needle is an effective tool for providing the sufficient tissue volume required for F1CDx.
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