Biallelic DNAH11 Variations Cause Male Infertility with Multiple Morphological Abnormalities of the Sperm Flagellum in Humans

Dear Editor,

Asthenoteratozoospermia, defined as reduced sperm motility and abnormal sperm morphology, accounts for approximately 19% of all male infertility. Multiple morphological abnormalities of the sperm flagellum (MMAF) is a severe type of asthenoteratozoospermia, characterized by a variety of sperm tail defects, including short, absent, curl, angle, or irregular sperm flagellum. MMAF is considered as a genetic disorder, with approximately 40 related genes identified so far, yet these genes account for only about 60% of human MMAF cases [1].

Motile cilia and sperm flagella share highly conserved axoneme with a “9 + 2” microtubule architecture. The inner dynein arms (IDAs) and outer dynein arms (ODAs) are two molecular motors to provide the original energy for ciliary beating and sperm motility in humans. DNAH family is involved in the formation of axoneme in motile cilia and sperm flagella [1]. DNAH11 encodes an ODA protein, and mutations in DNAH11 are known to cause primary ciliary dyskinesia (PCD) [2]. However, the role of DNAH11 in sperm motility and morphology has rarely been investigated.

In this study, we performed whole‑exome sequencing (WES) to investigate the potential genetic causes in two unrelated infertile patients with MMAF phenotype after ruling out conventional risk factors. Remarkably, two biallelic DNAH11 variations (NM_001277115.2) were identified, including a compound heterozygous mutation of c.6233G>A (p.Gly2078Glu) and c.9335A>G (p.Gln3112Arg) in patient A and another compound heterozygous mutation of c.6143C>G (p.Thr2048Arg) and c.10379C>A (p.Thr3460Lys) in patient B (Figure 1A). In addition, these variants were completely absent or presented at an exceedingly low frequency in the ExAC Browser (0, 0.0000352, 0.0003, 0.0003, respectively), GnomAD (0, 0, 0.0002, 0.0002, respectively), and 1000 Genomes Project databases (0, 0, 0.000399361, 0.000199681, respectively), predicted to be deleterious by bioinformatic tools, including SIFT, PolyPhen-2, and M-CAP. Moreover, the affected sites of theses variants are quite conserved across different species (Figure 1A).

Papanicolaou staining and scanning electron microscopy (SEM) analysis were carried out to evaluate the aberrant sperm morphology of the patients. Notably, the two patients presented a typical MMAF phenotype characterized by a combination of short, bent, coiled, absent, and/or irregular sperm flagellum (Figure 1B, i–iii). Moreover, the spermatozoa ultrastructure was analyzed by transmission electron microscopy (TEM). Compared with a normal sperm flagellum composed of a “9 + 2” axonemal arrangement from the control, the sperm flagella from the patients frequently exhibited the absence of central pairs (CPs), as well as disordered or missing peripheral microtubule doublet (MTDs) and outer dense fibers (ODFs) (Figure 1B, iv). The defects in mitochondrial sheath were also obvious in the sperm of the patients (Figure 1B, iv).

Notably, DNAH11 was expressed in the midpiece of the sperm tail of the control, while no DNAH11 signal was detected in the flagella of the sperm of the patients by immunofluorescence staining (Figure 1C). Compared with the control, the infertile patients exhibited diminished TOM20 signals by immunofluorescence staining (Figure 1C), indicating the defects of mitochondrial sheath in the sperm flagellum of the patients. Considering that DNAH11 encodes a motor protein of the ODAs, we performed immunofluorescence staining to assess whether the deficiency of DNAH11 affected the expression of other ODA-associated proteins. The results showed that in the control, DNAH8/DNAH17 and DNAI1, which correspond to the heavy and light intermediate chains of the ODAs, respectively, were primarily localized at the sperm flagella (Figure 1D). However, these proteins were almost absent in the spermatozoa of the patients (Figure 1D). We further found that the expression of key IDA-associated proteins of DNAH3/DNAH6 and DNALI1 in the sperm flagella of the patients was consistent with that of the control, indicating that the IDAs were not directly affected by DNAH11 deficiency (Figure 1D). Taken together, these data suggested that DNAH11 deficiency causing male infertility with MMAF may be related to a defect in ODA assembly resulting from the reduced expression of ODA-associated proteins.

In addition, intracytoplasmic sperm injection (ICSI) treatment was attempted for the DNAH11-mutated patients. The basal hormone data of the female partners were normal and ovulation induction was performed before oocyte retrieval. In the ICSI cycle of the patient A's wife, six Day 3 (D3) blastocyst embryos were obtained after standard embryo culture. The couple then underwent the transfer of two blastocysts and ultimately achieved clinical pregnancy. Unfortunately, during the ICSI cycle in the partner of the patient B, five available D3 embryos were obtained, but the implantation failed after two embryos transferred. Collectively, we suggested ICSI as an optional treatment for patients with DNAH11 variants and the failure of ICSI in patient B might be associated with other unidentified female factors.

Lucas et al previously demonstrated that the Dnah11^iv mice, carrying a missense (E2271K) mutation in the AAA2 domain of Dnahc11 (the mouse homolog of DNAH1), displayed reduced sperm motility [3]. For humans, only two studies have reported the single nucleotide polymorphisms of DNAH11 in patients with asthenozoospermia, suggesting that DNAH11 mutations may be a risk factor for male infertility [4, 5]. However, the exact connection and the underlying mechanism between DNAH11 mutations and sperm flagellar defects remain largely unknown in humans. Intriguingly, our study first identified two biallelic mutations in DNAH11 in two unrelated infertile patients with an MMAF phenotype, who denied suffering from any symptoms of PCD. Further investigation revealed the downregulated expression of ODA-associated proteins in the spermatozoa flagella of the patients. Taken together, our findings suggested that mutations in DNAH11 might affect ODAs assembly in human sperm tail and further result in an MMAF phenotype.

Notably, previous studies showed that ultrastructural defects of the ODA were not detected in cilia of most PCD patients with DNAH11 variants by conventional TEM [2]. However, the subtle ODA defects with abnormal ciliary beat patterns, hyperkinetic ciliary beating and reduced beating amplitude were visualized by high-speed video microscopy in these patients [2]. Specifically, high-speed video microscopy revealed a deficiency of >25% in the proximal ODA volume within the proximal ciliary region in DNAH11 mutation patients [2]. Reasonably, in our study, obvious ODA defects were not detected in sperm flagella of the patients by conventional TEM. However, this does not imply the absence of subtle defects, which might be visualized using high-speed video microscopy.

The clinical ICSI outcomes of MMAF patients have varied greatly due to differences in the pathogenic genetic etiologies. For example, successful ICSI outcomes for MMAF patients caused by mutations of DNAH1, DNAH2, and DNAH8 have been reported [1]. However, MMAF men harboring mutations in DNAH17 exhibited poor embryo quality after ICSI treatment. In this study, one patient with DNAH11 variants achieved successful outcomes with ICSI, while another patient experienced poor ICSI results. Certainly, additional female risk factors should not be overlooked. Collectively, further studies are required to explore the relationship between the ICSI outcomes and DNAH11 variants.

In conclusion, our study first identified two biallelic DNAH11 variations as a new genetic factor of MMAF and further provided adequate genetic and therapeutic counselling for male infertility. More cases and further studies using knock-in mice that mimic the DNAH11 mutations found in humans to demonstrate the exact pathogenic mechanism between DNAH11 variants and MMAF phenotypes are urgently warranted.

X. Y. performed the most of experiments. DM. L. and YH. Y. collected samples from human and collected the clinical data. GC. Z. conducted the clinical evaluations and wrote the original draft. GC. Z. and Y. S. conceptualization and reviewed the manuscript. Y. S. supervised the study. All authors critically reviewed and approved the final version of the manuscript.

This study was approved by the Ethics Committee of West China Second University Hospital (2020053). All subjects gave informed consent to participate in the study before taking part.

The authors declare no conflict of interest.