利用阿尔及利亚特有的阿尔布卡阿米娜(Batt.)的生物活性潜力。J.C. Manning和Goldblatt(天冬酰胺科):抗氧化活性、酶抑制和植物化学特征的新见解

IF 2.2 4区 化学 Q2 Engineering
Djihad Hamdani, Houari Benamar, Malika Bennaceur, Amina Chouh, Chawki Bensouici
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引用次数: 0

摘要

一种草本植物的球茎。J.C. Manning和Goldblatt传统上被阿尔及利亚当地居民用于对抗蛇咬伤、蝎子蜇伤和利什曼病。阿米娜在传统医学中也被用于治疗黑色素瘤和各种皮肤感染,并在止痛或兴奋的镇静水平上使用。这种植物含有生物活性化合物,如酚类物质和生物碱。本工作旨在探索阿米娜作为一种潜在的生物活性分子来源在食品和制药方面的应用。为此,从该草本植物中获得乙醇粗提物和馏分(氯仿、乙酸乙酯、正丁醇和水),并评估其对2,2-二苯基-1-吡啶肼(DPPH)、2,2 ' -氮基-二(3-乙基苯并噻唑-6-磺酸)(ABTS)和OH自由基的清除活性(RSA)、铁还原抗氧化能力(FRAP)、银离子(Ag+)还原电位和铁离子基总抗氧化能力。还研究了提取物对神经系统疾病发病相关酶(丁酰胆碱酯酶:BuChE)和2型糖尿病(T2DM, α-淀粉酶)的体外抑制作用。用比色法测定其总酚类物质(TPC)、总黄酮(TFC)和单宁(TTC)含量。采用超高效液相色谱-电喷雾电离串联质谱(UHPLC-ESI/MSMS)对该药材进行定性分析。乙酸乙酯组分具有显著的RSA和还原铁、银离子(Ag+)的能力。提取物对BuChE和α-淀粉酶具有较高的活性,特别是乙酸乙酯和正丁醇组分的乙醇粗提取物。正丁醇部位TPC最高(386.69 mg GAE/g DE),类黄酮含量最高(34.59 CE/g DE),乙酸乙酯部位TTC最高(263.28 mg CE/g DE)。UHPLC-ESI/MSMS在乙酸乙酯和正丁醇馏分中鉴定出19个化合物。其中甾类化合物1个,萜类化合物2个,脂肪酸1个,酚酸衍生物4个,depsidone 1个,其余为黄酮类化合物。结果表明,阿米娜是一种具有生物活性分子的有前景的食品、制药和生物医学应用来源。图形抽象
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Harnessing the bioactive potential of the Algerian endemic Albuca amoena (Batt.) J.C. Manning and Goldblatt (Asparagaceae): new insights into antioxidant activity, enzyme inhibition, and phytochemical profile

The bulbs of the herb Albuca amoena (Batt.) J.C. Manning and Goldblatt are traditionally used by the local populations in Algeria to counteract snakebites, scorpion stings, and leishmaniasis. A. amoena has also been used in traditional medicine to treat melanoma and various dermal infections and at the sedative level against pain or excitement. This plant contains bioactive compounds, such as phenolics and alkaloids. This work aimed to explore A. amoena as a potential source of bioactive molecules with food and pharmaceutical applications. For that purpose, ethanol crude extract and fractions (chloroform, ethyl acetate, n-butanol and aqueous) were obtained from the whole plant of this herb and evaluated for radical-scavenging activity (RSA) on 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and OH radicals, ferric-reducing antioxidant power (FRAP), silver ions (Ag+)-reducing potential, and for ferric-ion-based total antioxidant capacity. Extracts were also studied for in vitro inhibition of enzymes implicated on the onset of neurological diseases (butyrylcholinesterase: BuChE) and type 2 diabetes mellitus (T2DM, α-amylase). The total contents of phenolics (TPC), flavonoids (TFC), and tannins (TTC) were determined by colorimetric methods. Qualitative analyses of this herb were performed by ultra-high-performance liquid chromatography system coupled with electrospray ionization tandem mass spectrometry (UHPLC-ESI/MSMS). The ethyl acetate fraction had significant RSA and capacity to reduce iron, and silver ions (Ag+). The extracts had a higher activity toward BuChE and α-amylase, especially the ethanol crude extract with ethyl acetate and n-butanol fractions. The n-butanol fraction had the highest TPC (386.69 mg GAE/g DE) and flavonoid levels (34.59 CE/g DE), whereas the ethyl acetate fraction had the highest TTC (263.28 mg CE/g DE). UHPLC-ESI/MSMS identified 19 compounds in ethyl acetate and n-butanol fractions. Among these, one was steroid, two were terpenoids, one was fatty acid, four were phenolic acid derivatives, one was depsidone, and the remaining compounds were flavonoids. Results suggest A. amoena as a promising source of biologically active molecules with food, pharmaceutical, and biomedical applications.

Graphical abstract

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来源期刊
Chemical Papers
Chemical Papers Chemical Engineering-General Chemical Engineering
CiteScore
3.30
自引率
4.50%
发文量
590
期刊介绍: Chemical Papers is a peer-reviewed, international journal devoted to basic and applied chemical research. It has a broad scope covering the chemical sciences, but favors interdisciplinary research and studies that bring chemistry together with other disciplines.
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