根际细菌群落赋予蒙古黄芪耐旱性

IF 5.9 1区 农林科学 Q1 AGRONOMY
Shuyan Li, Peirong Li, Hao Ding, Simin Zhao, Jiamin Ai, Gehong Wei, Zhefei Li
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引用次数: 0

摘要

干旱胁迫显著抑制了黄芪的生长,导致黄芪生物量减少、光合效率降低、氧化损伤加剧。在我们的研究中,中度干旱胁迫下黄芪根中皂苷和黄酮类化合物的积累明显增加。这些次生代谢物进一步重塑了根际微生物群落结构,显著增加了其多样性和相互作用网络的复杂性。值得注意的是,干旱胁迫使根际土壤中有益菌属如根瘤菌和假单胞菌富集。结合可培养微生物的分离和根际细菌群落共现网络,构建了13株合成群落(SynCom),并将其简化为7株。与未接种对照相比,在中度干旱胁迫下,简化后的SynCom接种显著提高了植株生长,地上鲜重提高50.10 %,干重提高55.29 %,地下鲜重提高76.40 %。与未接种对照相比,处理过的植株地上鲜重和干重均显著增加,分别增加46.98 %和61.54 %。此外,接种简化菌群显著降低了黄芪丙二醛(MDA)含量,提高了过氧化氢酶(CAT)和过氧化物酶(POD)活性和叶片光合参数(Fv/Fm和Y(II))。本研究结果为提高黄芪的产量和品质提供了新的思路,并强调了合成根际微生物群落在帮助植物应对非生物胁迫方面的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Rhizosphere bacterial community confers drought tolerance to Astragalus mongholicus
Drought stress significantly inhibits the growth of Astragalus mongholicus, leading to reduced biomass, decreased photosynthetic efficiency, and exacerbated oxidative damage. In our study, the accumulation of saponins and flavonoids in Astragalus roots markedly increased under moderate drought stress. These secondary metabolites further reshaped the rhizosphere microbial community structure, significantly increasing its diversity and interaction network complexity. Notably, drought stress enriched beneficial bacterial genera such as Rhizobium and Pseudomonas in the rhizosphere soil. Combined with the isolation of culturable microorganisms and the co-occurrence network of the rhizosphere bacterial community, we constructed a 13-strain synthetic community (SynCom) and simplified it to 7 strains. Compared with the noninoculated control, under moderate drought stress, inoculation with the simplified SynCom significantly increased plant growth, increasing the aboveground fresh weight by 50.10 %, dry weight by 55.29 %, and underground fresh weight by 76.40 %. Similarly, plants treated with the synthetic community presented significant increases in aboveground fresh weight and dry weight compared with those of the noninoculated control, with increases of 46.98 % and 61.54 %, respectively. Moreover, inoculation with the simplified community significantly reduced the content of malondialdehyde (MDA) and improved the catalase (CAT) and peroxidase (POD) activities and leaf photosynthetic parameters (Fv/Fm and Y(II)) of Astragalus. Our findings provide new insight into improving the yield and quality of Astragalus and highlight the potential of synthetic rhizosphere microbial communities for assisting plants in coping with abiotic stress.
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来源期刊
Agricultural Water Management
Agricultural Water Management 农林科学-农艺学
CiteScore
12.10
自引率
14.90%
发文量
648
审稿时长
4.9 months
期刊介绍: Agricultural Water Management publishes papers of international significance relating to the science, economics, and policy of agricultural water management. In all cases, manuscripts must address implications and provide insight regarding agricultural water management.
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