不同马黛茶基因型生物活性物质的提取及抗菌凝胶的研制

IF 2.5 3区医学 Q3 CHEMISTRY, MEDICINAL

Fitoterapia Pub Date : 2025-05-02 DOI:10.1016/j.fitote.2025.106582

Gustavo Zambon Mendonça , Charles Windson Isidoro Haminiuk , Alessandra Cristina Pedro , Lucia Regina Rocha Martins , Fernando Molin , Gabriel Oales Kaminski Larsen , Cristiane Vieira Helm , Ivar Wendling , Giselle Maria Maciel

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引用次数: 0

摘要

巴拉圭茶（巴拉圭冬青）是生物活性化合物的重要来源，如多酚和甲基黄嘌呤，它们提供健康益处并具有潜在的抗菌特性。然而，马黛茶的基因型和获得植物提取物的条件可能会影响其生物学潜力的结果。本文采用统计实验设计，描述了从两种不同基因型马茶（BRS和EC38）中提取氢乙醇提取物的生产。测定各提取物的总酚类化合物（TPC）、总黄酮和黄酮醇（TFF）、抗氧化活性和抗菌潜力。选取部分提取物进行色谱评价（LC-DAD和LC-QTOF-MS）和抗菌凝胶的制备。TPC浓度以BRS基因型最高（112 mg GAE/g DW）， TFF浓度以EC38基因型最高（19.45 QE/g DW）。BRS和EC38的抗氧化活性最高，分别为598.9 μmol TE/g DW和753.7 μmol TE/g DW。hplc - qtof - ms共鉴定出16种酚类化合物和黄酮醇类化合物，以EC38基因型最多。与EC38基因型相比，BRS基因型提取物的可可碱和咖啡因含量较高。马黛茶提取物对大肠杆菌、金黄色葡萄球菌和白色念珠菌均有抑制作用，但抑制程度不同。与仅用乙醇（40%和70%）生产的洗手凝胶相比，马黛茶凝胶对大肠杆菌和金黄色葡萄球菌的抑制能力相同或更好。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Extraction of bioactive compounds from different yerba mate genotypes and development of an antimicrobial gel

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Extraction of bioactive compounds from different yerba mate genotypes and development of an antimicrobial gel

Yerba mate (Ilex paraguariensis) is an essential source of bioactive compounds, such as polyphenols and methylxanthines, which provide health benefits and present potential antimicrobial properties. Nevertheless, the yerba mate genotypes and conditions to obtain plant extracts may influence the results of their biological potential. This paper describes the production of hydroethanolic extracts from two different genotypes of yerba mate (BRS and EC38) using a statistical experimental design. The total phenolic compounds (TPC), total flavones and flavonols (TFF), antioxidant activity, and antimicrobial potential were determined for each extract. Besides, some extracts were selected for chromatographic evaluation (LC-DAD and LC-QTOF-MS) and the development of an antimicrobial gel. The highest concentration of TPC was observed in BRS genotype (112 mg GAE/g DW) and the highest value of TFF was detected in EC38 genotype (19.45 QE/g DW). The highest values of antioxidant activities were 598.9 μmol TE/g DW for BRS and 753.7 μmol TE/g DW for EC38. A total of 16 phenolic compounds and flavonols were identified in yerba mate extracts by LC-QTOF-MS, and the EC38 genotype presented the highest number of compounds. The extracts from the BRS genotype had higher concentration of theobromine and caffeine, compared to the EC38 genotype. The yerba mate extracts could inhibit Escherichia coli, Staphylococcus aureus and Candida albicans, though at different levels. The yerba mate gels demonstrated equal and/or superior inhibitory capacity against E. coli and S. aureus compared to hand sanitizing gel produced only with ethanol (40 % and 70 %).

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来源期刊

Fitoterapia 医学-药学

CiteScore

5.80

自引率

2.90%

发文量

198

审稿时长

1.5 months

期刊介绍： Fitoterapia is a Journal dedicated to medicinal plants and to bioactive natural products of plant origin. It publishes original contributions in seven major areas: 1. Characterization of active ingredients of medicinal plants 2. Development of standardization method for bioactive plant extracts and natural products 3. Identification of bioactivity in plant extracts 4. Identification of targets and mechanism of activity of plant extracts 5. Production and genomic characterization of medicinal plants biomass 6. Chemistry and biochemistry of bioactive natural products of plant origin 7. Critical reviews of the historical, clinical and legal status of medicinal plants, and accounts on topical issues.