骨痹颗粒治疗骨关节炎的药效学研究

Yingxia Bao , Liyu Hao , Yiqiu Liao , Jianhui Sun , Yiman Chen , Jianliang Li , Hongmei Li , Lingli Wang , Jiansong Wang
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引用次数: 0

摘要

骨关节炎(OA)是一种与年龄相关的退行性疾病,主要特征是关节僵硬、疼痛和关节软骨的进行性破坏。骨痹颗粒是治疗骨性关节炎的经典医院制剂。方法建立西班牙毒素诱导的大鼠和改良的大鼠Hulth模型,观察骨痹颗粒的保护作用。通过苏木精和伊红(H&;E)染色观察大鼠软骨组织的病理改变,并用Mankin评分进行定量评分。采用市售试剂盒测定大鼠血清中PGE2和iNOS水平。采用巴豆油致小鼠耳肿胀模型、卡拉胶致大鼠足肿胀模型和棉球致大鼠肉芽肿模型研究骨痹颗粒的抗炎作用。并通过小鼠醋酸扭体实验评价其镇痛作用。结果骨痹颗粒能明显减轻大鼠骨关节组织病理改变,显著降低关节Mankin评分和关节肿胀程度(P <;0.01)。此外,骨痹颗粒可显著降低血清PGE2和iNOS水平(P <;0.01)。骨痹颗粒对小鼠耳肿胀有明显抑制作用(P <;0.05),大鼠足跖肿胀及棉球致肉芽肿(P <;0.01),并显著抑制小鼠扭体反应(P <;0.01)。结论骨痹颗粒具有明显的抗炎镇痛作用,对骨关节炎具有明显的治疗作用,为骨痹颗粒在骨关节炎治疗中的临床应用提供了实验依据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Pharmacodynamic study of Gubi granules in the treatment of osteoarthritis

Background

Osteoarthritis (OA) is an aging-associated degenerative disease, primarily characterized by joint stiffness pain and progressive destruction of articular cartilage. Gubi granules is a classic hospital-prepared formulation specifically for the treatment of OA.

Methods

Papain-induced and modified Hulth models were established in rats to investigate the protective effects of Gubi granules. Pathological alterations in rat cartilage tissues were observed through hematoxylin and eosin (H&E) staining, and then quantitatively scored using the Mankin score. Commercially available kits were employed to measure the levels of PGE2 and iNOS in rat serum. The anti-inflammatory effects of Gubi Granules were investigated by croton-oil-induced ear swelling model in mice, carrageenan-induced paw swelling and cotton-pellet-induced granuloma models in rats. Moreover, the analgesic effect was evaluated by the acetic acid-induced writhing test in mice.

Results

The results showed that Gubi granules significantly alleviated the pathological changes of osteoarticular tissues, and remarkably decreased the Mankin score of joints and the extent of joint swelling (P < 0.01). Additionally, Gubi Granules led to a significant reduction in the serum levels of PGE2 and iNOS (P < 0.01). Moreover, Gubi granules significantly supressed ear swelling in mice (P < 0.05), paw swelling and cotton-pellet-induced granuloma in rats (P < 0.01), as well as significantly inhibited the writhing response in mice (P < 0.01).

Conclusion

These findings indicate that Gubi granules exhibit notable anti-inflammatory and analgesic properties, and demonstrate a significant therapeutic efficacy against osteoarthritis, Overall, these results provide an experimental basis to support the clinical application of Gubi Granules in the management of osteoarthritis.
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