Yingxia Bao , Liyu Hao , Yiqiu Liao , Jianhui Sun , Yiman Chen , Jianliang Li , Hongmei Li , Lingli Wang , Jiansong Wang
{"title":"骨痹颗粒治疗骨关节炎的药效学研究","authors":"Yingxia Bao , Liyu Hao , Yiqiu Liao , Jianhui Sun , Yiman Chen , Jianliang Li , Hongmei Li , Lingli Wang , Jiansong Wang","doi":"10.1016/j.prmcm.2025.100621","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><div>Osteoarthritis (OA) is an aging-associated degenerative disease, primarily characterized by joint stiffness pain and progressive destruction of articular cartilage. Gubi granules is a classic hospital-prepared formulation specifically for the treatment of OA.</div></div><div><h3>Methods</h3><div>Papain-induced and modified Hulth models were established in rats to investigate the protective effects of Gubi granules. Pathological alterations in rat cartilage tissues were observed through hematoxylin and eosin (H&E) staining, and then quantitatively scored using the Mankin score. Commercially available kits were employed to measure the levels of PGE2 and iNOS in rat serum. The anti-inflammatory effects of Gubi Granules were investigated by croton-oil-induced ear swelling model in mice, carrageenan-induced paw swelling and cotton-pellet-induced granuloma models in rats. Moreover, the analgesic effect was evaluated by the acetic acid-induced writhing test in mice.</div></div><div><h3>Results</h3><div>The results showed that Gubi granules significantly alleviated the pathological changes of osteoarticular tissues, and remarkably decreased the Mankin score of joints and the extent of joint swelling (<em>P</em> < 0.01). Additionally, Gubi Granules led to a significant reduction in the serum levels of PGE2 and iNOS (<em>P</em> < 0.01). Moreover, Gubi granules significantly supressed ear swelling in mice (<em>P</em> < 0.05), paw swelling and cotton-pellet-induced granuloma in rats (<em>P</em> < 0.01), as well as significantly inhibited the writhing response in mice (<em>P</em> < 0.01).</div></div><div><h3>Conclusion</h3><div>These findings indicate that Gubi granules exhibit notable anti-inflammatory and analgesic properties, and demonstrate a significant therapeutic efficacy against osteoarthritis, Overall, these results provide an experimental basis to support the clinical application of Gubi Granules in the management of osteoarthritis.</div></div>","PeriodicalId":101013,"journal":{"name":"Pharmacological Research - Modern Chinese Medicine","volume":"15 ","pages":"Article 100621"},"PeriodicalIF":0.0000,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Pharmacodynamic study of Gubi granules in the treatment of osteoarthritis\",\"authors\":\"Yingxia Bao , Liyu Hao , Yiqiu Liao , Jianhui Sun , Yiman Chen , Jianliang Li , Hongmei Li , Lingli Wang , Jiansong Wang\",\"doi\":\"10.1016/j.prmcm.2025.100621\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><div>Osteoarthritis (OA) is an aging-associated degenerative disease, primarily characterized by joint stiffness pain and progressive destruction of articular cartilage. Gubi granules is a classic hospital-prepared formulation specifically for the treatment of OA.</div></div><div><h3>Methods</h3><div>Papain-induced and modified Hulth models were established in rats to investigate the protective effects of Gubi granules. Pathological alterations in rat cartilage tissues were observed through hematoxylin and eosin (H&E) staining, and then quantitatively scored using the Mankin score. Commercially available kits were employed to measure the levels of PGE2 and iNOS in rat serum. The anti-inflammatory effects of Gubi Granules were investigated by croton-oil-induced ear swelling model in mice, carrageenan-induced paw swelling and cotton-pellet-induced granuloma models in rats. Moreover, the analgesic effect was evaluated by the acetic acid-induced writhing test in mice.</div></div><div><h3>Results</h3><div>The results showed that Gubi granules significantly alleviated the pathological changes of osteoarticular tissues, and remarkably decreased the Mankin score of joints and the extent of joint swelling (<em>P</em> < 0.01). Additionally, Gubi Granules led to a significant reduction in the serum levels of PGE2 and iNOS (<em>P</em> < 0.01). Moreover, Gubi granules significantly supressed ear swelling in mice (<em>P</em> < 0.05), paw swelling and cotton-pellet-induced granuloma in rats (<em>P</em> < 0.01), as well as significantly inhibited the writhing response in mice (<em>P</em> < 0.01).</div></div><div><h3>Conclusion</h3><div>These findings indicate that Gubi granules exhibit notable anti-inflammatory and analgesic properties, and demonstrate a significant therapeutic efficacy against osteoarthritis, Overall, these results provide an experimental basis to support the clinical application of Gubi Granules in the management of osteoarthritis.</div></div>\",\"PeriodicalId\":101013,\"journal\":{\"name\":\"Pharmacological Research - Modern Chinese Medicine\",\"volume\":\"15 \",\"pages\":\"Article 100621\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2025-04-28\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Pharmacological Research - Modern Chinese Medicine\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2667142525000508\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Pharmacological Research - Modern Chinese Medicine","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2667142525000508","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Pharmacodynamic study of Gubi granules in the treatment of osteoarthritis
Background
Osteoarthritis (OA) is an aging-associated degenerative disease, primarily characterized by joint stiffness pain and progressive destruction of articular cartilage. Gubi granules is a classic hospital-prepared formulation specifically for the treatment of OA.
Methods
Papain-induced and modified Hulth models were established in rats to investigate the protective effects of Gubi granules. Pathological alterations in rat cartilage tissues were observed through hematoxylin and eosin (H&E) staining, and then quantitatively scored using the Mankin score. Commercially available kits were employed to measure the levels of PGE2 and iNOS in rat serum. The anti-inflammatory effects of Gubi Granules were investigated by croton-oil-induced ear swelling model in mice, carrageenan-induced paw swelling and cotton-pellet-induced granuloma models in rats. Moreover, the analgesic effect was evaluated by the acetic acid-induced writhing test in mice.
Results
The results showed that Gubi granules significantly alleviated the pathological changes of osteoarticular tissues, and remarkably decreased the Mankin score of joints and the extent of joint swelling (P < 0.01). Additionally, Gubi Granules led to a significant reduction in the serum levels of PGE2 and iNOS (P < 0.01). Moreover, Gubi granules significantly supressed ear swelling in mice (P < 0.05), paw swelling and cotton-pellet-induced granuloma in rats (P < 0.01), as well as significantly inhibited the writhing response in mice (P < 0.01).
Conclusion
These findings indicate that Gubi granules exhibit notable anti-inflammatory and analgesic properties, and demonstrate a significant therapeutic efficacy against osteoarthritis, Overall, these results provide an experimental basis to support the clinical application of Gubi Granules in the management of osteoarthritis.
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