Evaluation of the efficacy and safety of omega-3 fatty acid nutritional supplements from Schizochytrium sp. in dog food

The rising demand for omega-3 polyunsaturated fatty acids (n-3 PUFAs) and concerns over fish oil sustainability have driven the search for alternatives. This study evaluated the efficacy and safety of algal powder and algal oil from Schizochytrium sp. as n-3 PUFAs supplements in dogs, compared to fish oil. Twenty-four Beagle dogs were fed diets supplemented with 1 % fish oil, 1 % algal powder, 1 % algal oil, or no n-3 PUFAs for 28 days. Results showed that algal-derived supplements significantly increased serum DHA levels (up to 0.265 ± 0.05 g/l), improved coat quality, and enhanced antioxidant capacity. Notably, algal powder exhibited the strongest lipid-lowering effects, reducing total cholesterol to 69.4 % of control levels, despite its lower EPA content. Safety function markers (alanine transferase, alkaline phosphatase, and acid phosphatase) remained stable across all groups, confirming safety. These findings highlight algal powder and algal oil as effective, safe, and sustainable n-3 PUFA sources, offering a viable alternative to fish oil for canine nutrition.