半纤维素衍生的人类蛋白的进化过程

IF 7.3 1区 化学 Q1 CHEMISTRY, MULTIDISCIPLINARY
Xugang Ye, Xiaoxiang Li, Kai Hu, Li Liu
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引用次数: 0

摘要

作为木质纤维素的三组分之一,半纤维素的转化受到人源蛋白的严重阻碍。本研究以玉米芯木聚糖为模型,研究半纤维素合成人蛋白的机理(产量53.4%),透析分离出关键可溶性中间体9,产率0.15%。此外,通过HPLC-MS/MS、IR、13C固体核磁共振、凝胶渗透色谱(GPC)、MALDI-TOF和SEM分析,提出木聚糖类人敏化合物的形成涉及6种基本反应,包括解聚、醚化、亲电取代、醛醇缩合、脱水和热氧化。在早期,木聚糖解聚得到木糖,木糖通过小分子机制不断脱水和醚化形成早期人类素,并伴随着糠醛/糠醇(FAL)的亲电取代和糠醛片段与乙酰丙酸(LA)的醛醇缩合。在后期,来自木聚糖解聚的低聚木糖经过醚化反应形成更大的多糖,通过低聚物机制将其纳入人体。同时,脱水和热氧化程度加深,导致C = C和C = O的结合增强。首次通过原子力显微镜(AFM)进一步可视化了低聚物的机制,为人类在单分子水平上的进化提供了新的途径。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Evolution Process of Humins Derived from Hemicellulose

Evolution Process of Humins Derived from Hemicellulose
As one of the three components in lignocellulose, the conversion of hemicellulose was seriously hindered by the formation of humins. In this study, xylan from corn cob was used as the model to study the formation mechanism of humins from hemicellulose (53.4% in yield), whereas a key soluble intermediate of 9 was separated by dialysis with a yield of 0.15%. Furthermore, by means of HPLC-MS/MS analysis, IR, 13C solid-state NMR, gel permeation chromatography (GPC), MALDI-TOF, and SEM, it was proposed that six types of elementary reactions were involved in the formation of xylan-derived humins, including depolymerization, etherification, electrophilic substitution, aldol condensation, dehydration, and thermal oxidation. In the early stage, xylan depolymerization results in xylose, which undertakes continuous dehydration and etherification to form the early humins via the small-molecule mechanism, accompanied by electrophilic substitution of furfural/furfuryl alcohol (FAL) and aldol condensation of the furfural fragment with levulinic acid (LA). In the later stage, xylo-oligosaccharides from xylan depolymerization undergo etherification to form larger polysaccharides, which are incorporated into the humins via the oligomer mechanism. Meanwhile, the degree of dehydration and thermal oxidation deepens, resulting in the enhancement of C═C and C═O conjugation. For the first time, the oligomer mechanism was further visualized by atomic force microscopy (AFM), demonstrating a new approach of humin evolution at the single-molecule level.
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来源期刊
ACS Sustainable Chemistry & Engineering
ACS Sustainable Chemistry & Engineering CHEMISTRY, MULTIDISCIPLINARY-ENGINEERING, CHEMICAL
CiteScore
13.80
自引率
4.80%
发文量
1470
审稿时长
1.7 months
期刊介绍: ACS Sustainable Chemistry & Engineering is a prestigious weekly peer-reviewed scientific journal published by the American Chemical Society. Dedicated to advancing the principles of green chemistry and green engineering, it covers a wide array of research topics including green chemistry, green engineering, biomass, alternative energy, and life cycle assessment. The journal welcomes submissions in various formats, including Letters, Articles, Features, and Perspectives (Reviews), that address the challenges of sustainability in the chemical enterprise and contribute to the advancement of sustainable practices. Join us in shaping the future of sustainable chemistry and engineering.
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