芦皮醇对农药致雄性大鼠睾丸及氧化损伤的保护作用

IF 2.9 4区 生物学 Q3 CELL BIOLOGY
Pınar Koroglu, Ismet Burcu Turkyılmaz Mutlu, Huseyin Us, Melis Coremen, Ayca Sezen Us, Omur Karabulut Bulan, Refiye Yanardag
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引用次数: 0

摘要

农药在农业中用作杀虫剂。Lupeol (LUP)是一种具有抗氧化作用的膳食三萜。本实验旨在通过组织学和生化研究LUP对睾丸农药毒性的影响。采用Wistar白化大鼠。对照、玉米油、马拉硫磷(MAL)、毒死蜱(CPF)、戊康唑(TEB)、LUP、MAL + LUP、CPF + LUP和TEB + LUP组。对照组大鼠灌胃生理盐水。玉米油作为溶剂用于农药制剂的制备。经灌胃给予MAL 50 mg/kg, CPF 10 mg/kg, TEB 50 mg/kg(前4天)和25 mg/kg(后4天),LUP 20 mg/kg。10 d后解剖大鼠,取睾丸进行组织学分析。采用分光光度法测定睾丸组织样品的氧化应激参数。农药组组织学和生化损伤评分升高,精子涂片出现形态学缺陷。这些缺陷在给予LUP后被逆转。LUP对农药致睾丸损伤的组织病理学和生化指标均有改善作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Protective effects of lupeol on pesticides induced testicular and oxidative damage of male rats

Pesticides are used as insecticides in agriculture. Lupeol (LUP) is a dietary triterpene with antioxidant effects. This experiment aimed to investigate the effects of LUP against testicular pesticides toxicity via histological and biochemical findings. Wistar albino rats were used. Control, corn oil, malathion (MAL), chlorpyrifos (CPF), tebuconazole (TEB), LUP, MAL + LUP, CPF + LUP, and TEB + LUP group. Control group rats were given physiological saline. Corn oil is used as a solvent in the preparation of pesticide agents. MAL 50 mg/kg, CPF 10 mg/kg, TEB 50 mg/kg (first 4 days) and 25 mg/kg (last 4 days), LUP 20 mg/kg were given via oral gavage. After 10 days, rats were dissected, and testes were taken for histological analysis. Oxidative stress parameters were determined spectrophotometrically in testicular tissue specimens. In the pesticide group, histological and biochemical damage score increased, morphological sperm smear defects were also detected. These defects were reversed upon the administration of LUP. LUP demonstrated an ameliorative effect on histopathological and biochemical parameters in pesticide induced testicular damage.

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来源期刊
Journal of Molecular Histology
Journal of Molecular Histology 生物-细胞生物学
CiteScore
5.90
自引率
0.00%
发文量
68
审稿时长
1 months
期刊介绍: The Journal of Molecular Histology publishes results of original research on the localization and expression of molecules in animal cells, tissues and organs. Coverage includes studies describing novel cellular or ultrastructural distributions of molecules which provide insight into biochemical or physiological function, development, histologic structure and disease processes. Major research themes of particular interest include: - Cell-Cell and Cell-Matrix Interactions; - Connective Tissues; - Development and Disease; - Neuroscience. Please note that the Journal of Molecular Histology does not consider manuscripts dealing with the application of immunological or other probes on non-standard laboratory animal models unless the results are clearly of significant and general biological importance. The Journal of Molecular Histology publishes full-length original research papers, review articles, short communications and letters to the editors. All manuscripts are typically reviewed by two independent referees. The Journal of Molecular Histology is a continuation of The Histochemical Journal.
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