去除真皮乳头的自体毛囊移植促进巴马迷你猪伤口修复和再生

IF 2.9 4区 生物学 Q3 CELL BIOLOGY
Wenzi Liang, Chang Mai, Yuandong Yuan, Xiuwen Chen, Bozhi Cai, Na Ni, Chutong Zhuang, Changmin Lin, Keng Huang
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引用次数: 0

摘要

自体游离皮瓣移植是临床上治疗大面积创伤最经济、最有效的方法,但皮瓣供体的缺乏阻碍了该方法的广泛应用。毛囊干细胞具有修复伤口的巨大潜力,但毛囊干细胞修复伤口尚未达到预期效果。我们采用巴马迷你猪的创面模型,用自体毛囊或皮瓣治疗创面。术后第7、14、21天观察创面愈合情况,采用Image J软件分析创面愈合率。苏木精和伊红染色评价创面的再上皮化情况。采用免疫荧光染色法检测毛囊干细胞标志物CK15、Sox9的表达,探讨创面修复的机制。这项研究发现自体毛囊可以加速伤口愈合。毛囊在伤口修复中的作用与其结构有关。真皮乳头是毛囊介导的伤口修复的生物屏障。去除真皮乳头可提高伤口愈合效率,可能是通过缓解真皮乳头对毛囊干细胞迁移的限制。自体毛囊用于创面修复具有损伤小、制作简单、来源丰富等优点,有望在未来替代皮瓣移植作为一种治疗策略。本研究有助于阐明毛囊参与创面修复的调控机制,对解决皮瓣供体不足问题具有重要的学术和临床价值。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Autografted hair follicles with dermal papilla removed promote wound repair and regeneration in Bama mini-pigs

Autologous free flap transplantation is the most economical and effective method for clinical treatment of large-area trauma, but the shortage of flap donors prevents widespread use of this method. Hair follicle stem cells have great potential to repair wounds, but wound repair by hair follicle stem cells has not yet met expectations. We used a wound model of Bama mini-pigs and treated the wound with autologous hair follicles or flaps. The wound healing was observed on days 7, 14, and 21 post-surgery and wound healing rates were analyzed using Image J software. Hematoxylin and eosin staining was performed to evaluate re-epithelialization of the wound. Immunofluorescence staining was used to detect the expression of hair follicle stem cell markers (CK15, Sox9) and explore the mechanism of wound repair. This research found that autologous hair follicles can accelerate wound healing. The efficiency of hair follicles in wound repair is related to their structure. Dermal papilla acts as a biological barrier to hair follicle-mediated wound repair. Dermal papilla removal enhances wound healing efficiency, likely by relieving dermal papilla-imposed restrictions on hair follicle stem cell migration. Autologous hair follicles for wound repair has the advantages of minimal damage, simple fabrication, and abundant source, which may be able to replace the flap transplantation as a therapeutic strategy in the future. This study is helpful to elucidate the regulatory mechanism of hair follicles involved in wound repair, and has important academic and clinical value for solving the problem of shortage flap donor.

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来源期刊
Journal of Molecular Histology
Journal of Molecular Histology 生物-细胞生物学
CiteScore
5.90
自引率
0.00%
发文量
68
审稿时长
1 months
期刊介绍: The Journal of Molecular Histology publishes results of original research on the localization and expression of molecules in animal cells, tissues and organs. Coverage includes studies describing novel cellular or ultrastructural distributions of molecules which provide insight into biochemical or physiological function, development, histologic structure and disease processes. Major research themes of particular interest include: - Cell-Cell and Cell-Matrix Interactions; - Connective Tissues; - Development and Disease; - Neuroscience. Please note that the Journal of Molecular Histology does not consider manuscripts dealing with the application of immunological or other probes on non-standard laboratory animal models unless the results are clearly of significant and general biological importance. The Journal of Molecular Histology publishes full-length original research papers, review articles, short communications and letters to the editors. All manuscripts are typically reviewed by two independent referees. The Journal of Molecular Histology is a continuation of The Histochemical Journal.
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