两种水通道蛋白LcPIP1；4和LcPIP1；4a协同调节常绿多年生荔枝（litchi chinensis Sonn.）顶芽的休眠。

IF 8.7 1区农林科学 Q1 Agricultural and Biological Sciences

Horticulture Research Pub Date : 2025-05-07 DOI:10.1093/hr/uhaf122

Xue Tian, Zhi-Qun Zhong, Yu Qi, Meng-Meng Ma, Ming-Chao Yang, Dong-Cheng Li, Fang-Yi Zhang, Hui-Cong Wang, Ji-Yuan Shen, Ren-Fang Zeng, Xu-Ming Huang

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引用次数: 0

摘要

虽然在各种植物中进行了广泛的研究，但水通道蛋白在荔枝中的作用尚不清楚。本研究发现，荔枝休眠顶芽中水分含量较低。转录组分析显示，外源乙烯（ETH）显著抑制了两个水通道蛋白基因，质膜内在蛋白1；4 （LcPIP1;4）和LcPIP1；5，这也降低了荔枝芽的水分含量。实时荧光定量PCR检测表明，LcPIP1；4在荔枝顶芽休眠阶段表达水平相对较高。在生长期抑制荔枝芽中LcPIP1；4，使休眠时间延迟，休眠率显著降低，水分含量显著增加。进一步研究表明，LcPIP1；4与LcPIP1；4a相互作用，并具有自相互作用能力。3 .荔枝芽中LcPIP1；4a的沉默导致表型与LcPIP1的沉默一致；此外，LcPIP1；4和LcPIP1；4a同时沉默导致更严重的芽休眠表型。LcRAP2.4直接上调LcPIP1；4。LcRAP2.4的沉默也延迟了荔枝顶芽休眠的开始，这是由LcSVP2调控的。1000mg /L ETH处理显著下调LcPIP1；4和LcRAP2.4的表达，但对LcPIP1；4a无显著影响。相比之下，200 mg/L ABA处理显著上调LcPIP1；4、LcPIP1；4a和LcRAP2.4的表达。ETH与ABA联合处理对芽的抑制作用更强，LcPIP1；4和LcRAP2.4的上调程度低于ABA单独处理，说明ABA逆转了ETH对LcPIP1；4和LcRAP2.4表达的抑制作用。ABA处理和ETH与ABA联合处理能有效降低顶芽水分含量。这些结果表明，LcRAP2、4、LcPIP1；4和LcPIP1；4a通过调节水分水平在荔枝顶芽休眠中起着至关重要的作用。

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Two aquaporins, LcPIP1;4 and LcPIP1;4a, cooperatively regulate the onset of dormancy of the terminal buds in evergreen perennial litchi (Litchi chinensis Sonn.)

Although extensively studied in various plants, the roles of aquaporin proteins in litchi remain unclear. In this study, low moisture content was observed in the dormant terminal buds of litchi. Transcriptome analysis revealed that two aquaporin genes, PLASMA MEMBRANE INTRINSIC PROTEIN 1;4 (LcPIP1;4) and LcPIP1;5, could be remarkably inhibited by exogenous ethylene (ETH), which also reduced the moisture content of litchi buds. Quantitative real-time PCR assays indicated that LcPIP1;4 expression was relatively elevated in the dormancy stage of litchi terminal buds. Inhibition of LcPIP1;4 in the buds of litchi during the growth stage delayed the onset of dormancy, resulting in a significantly reduced dormancy rate and increased moisture content. Further study indicated that LcPIP1;4 interacts with LcPIP1;4a, and they are capable of self-interaction. Silencing of LcPIP1;4a in litchi buds resulted in a phenotype consistent with silencing of LcPIP1;4. Additionally, simultaneous silencing of both LcPIP1;4 and LcPIP1;4a resulted in a more severe bud dormancy phenotype. Moreover, LcPIP1;4 was directly upregulated by LcRAP2.4. Silencing of LcRAP2.4 also delayed the onset of dormancy in litchi terminal buds, which is regulated by LcSVP2. ETH treatment at 1000 mg/L significantly downregulated the expression of LcPIP1;4 and LcRAP2.4, but had no significant effect on LcPIP1;4a. In contrast, ABA treatment at 200 mg/L significantly upregulated the expression of LcPIP1;4, LcPIP1;4a, and LcRAP2.4. Combined treatment with ETH and ABA exerted a stronger inhibitory effect on the budbreak and upregulated LcPIP1;4 and LcRAP2.4 to lower degrees than ABA alone, suggesting that ABA reversed the inhibitory effect of ETH on expression of LcPIP1;4 and LcRAP2.4. ABA treatment and combined treatment with ETH and ABA effectively reduced the moisture content of the terminal buds. These results demonstrate that LcRAP2,4, LcPIP1;4, and LcPIP1;4a play a vital role in dormancy onset of litchi terminal buds by regulating moisture levels.

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来源期刊

Horticulture Research Biochemistry, Genetics and Molecular Biology-Biochemistry

CiteScore

11.20

自引率

6.90%

发文量

367

审稿时长

20 weeks

期刊介绍： Horticulture Research, an open access journal affiliated with Nanjing Agricultural University, has achieved the prestigious ranking of number one in the Horticulture category of the Journal Citation Reports ™ from Clarivate, 2022. As a leading publication in the field, the journal is dedicated to disseminating original research articles, comprehensive reviews, insightful perspectives, thought-provoking comments, and valuable correspondence articles and letters to the editor. Its scope encompasses all vital aspects of horticultural plants and disciplines, such as biotechnology, breeding, cellular and molecular biology, evolution, genetics, inter-species interactions, physiology, and the origination and domestication of crops.