壳聚糖在股四头肌肌腱断裂一期修复后组织愈合中的作用：实验动物模型

IF 1.6 4区医学 Q3 ORTHOPEDICS

Knee Pub Date : 2025-05-08 DOI:10.1016/j.knee.2025.04.018

Tevfik Çatal , Alkan Bayrak , Ahmet Çanlıoğlu , Başar Burak Çakmur , Murat Tıngır , Alev Kural , Ahmet Gülçubuk , Gülay Yüzbaşıoğlu Öztürk , Cemal Kural , Altuğ Duramaz

{"title":"壳聚糖在股四头肌肌腱断裂一期修复后组织愈合中的作用：实验动物模型","authors":"Tevfik Çatal , Alkan Bayrak , Ahmet Çanlıoğlu , Başar Burak Çakmur , Murat Tıngır , Alev Kural , Ahmet Gülçubuk , Gülay Yüzbaşıoğlu Öztürk , Cemal Kural , Altuğ Duramaz","doi":"10.1016/j.knee.2025.04.018","DOIUrl":null,"url":null,"abstract":"<div><h3>Aim</h3><div>To expedite the healing of tendons by introducing chitosan to the repaired tendon site.</div></div><div><h3>Methods</h3><div>Thirty-six adult, male Wistar Albino rats (300–450 g) were randomly divided into three groups. A full-thickness quadriceps injury model was created in the right hind leg of all animals. The first group did not undergo any intervention (control group). In the second group, primary repair was performed following injury (primary suturing (PS) group). In the third group, primary repair was performed, and 50 mg chitosan was administered via injection at the repair site (PS+Chitosan group). Tail venous blood samples were collected from all rats on days 0, 3, 7 and 14. On days 14 and 28, four subjects from each group were sacrificed for histopathologic evaluation. On day 28, four subjects from each group were sacrificed for biomechanical investigation.</div></div><div><h3>Results</h3><div>The mean total value of the PS+Chitosan group on day 28 was lower than the mean total value of the control group on days 14 and 28 (<em>P</em> = 0.004). The mean IL-1β value on day 14 was higher in the PS group compared with the other groups (<em>P</em> < 0.001). The mean TGF-β value on day 14 was lower in the PS+Chitosan group compared with the other groups (<em>P</em> < 0.001). The average maximal tensile resistance in the monitor group was 1.51 N/mm<sup>2</sup>. Although statistically significant results were not found, the PS+Chitosan group exhibited biomechanical values that were most similar to those of the intact tendon, while the control group displayed the most divergent results.</div></div><div><h3>Conclusion</h3><div>Chitosan application accelerates tendon healing after repair in quadriceps tendon injuries in our rat model. Chitosan affects different pathways and enhances tendon healing as observed in our study.</div></div>","PeriodicalId":56110,"journal":{"name":"Knee","volume":"55 ","pages":"Pages 193-205"},"PeriodicalIF":1.6000,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"The role of chitosan in tissue healing after primary repair of quadriceps tendon ruptures: Experimental animal model\",\"authors\":\"Tevfik Çatal , Alkan Bayrak , Ahmet Çanlıoğlu , Başar Burak Çakmur , Murat Tıngır , Alev Kural , Ahmet Gülçubuk , Gülay Yüzbaşıoğlu Öztürk , Cemal Kural , Altuğ Duramaz\",\"doi\":\"10.1016/j.knee.2025.04.018\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Aim</h3><div>To expedite the healing of tendons by introducing chitosan to the repaired tendon site.</div></div><div><h3>Methods</h3><div>Thirty-six adult, male Wistar Albino rats (300–450 g) were randomly divided into three groups. A full-thickness quadriceps injury model was created in the right hind leg of all animals. The first group did not undergo any intervention (control group). In the second group, primary repair was performed following injury (primary suturing (PS) group). In the third group, primary repair was performed, and 50 mg chitosan was administered via injection at the repair site (PS+Chitosan group). Tail venous blood samples were collected from all rats on days 0, 3, 7 and 14. On days 14 and 28, four subjects from each group were sacrificed for histopathologic evaluation. On day 28, four subjects from each group were sacrificed for biomechanical investigation.</div></div><div><h3>Results</h3><div>The mean total value of the PS+Chitosan group on day 28 was lower than the mean total value of the control group on days 14 and 28 (<em>P</em> = 0.004). The mean IL-1β value on day 14 was higher in the PS group compared with the other groups (<em>P</em> < 0.001). The mean TGF-β value on day 14 was lower in the PS+Chitosan group compared with the other groups (<em>P</em> < 0.001). The average maximal tensile resistance in the monitor group was 1.51 N/mm<sup>2</sup>. Although statistically significant results were not found, the PS+Chitosan group exhibited biomechanical values that were most similar to those of the intact tendon, while the control group displayed the most divergent results.</div></div><div><h3>Conclusion</h3><div>Chitosan application accelerates tendon healing after repair in quadriceps tendon injuries in our rat model. Chitosan affects different pathways and enhances tendon healing as observed in our study.</div></div>\",\"PeriodicalId\":56110,\"journal\":{\"name\":\"Knee\",\"volume\":\"55 \",\"pages\":\"Pages 193-205\"},\"PeriodicalIF\":1.6000,\"publicationDate\":\"2025-05-08\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Knee\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0968016025000894\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"ORTHOPEDICS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Knee","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0968016025000894","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"ORTHOPEDICS","Score":null,"Total":0}

引用次数: 0

摘要

目的通过将壳聚糖引入修复后的肌腱部位，促进肌腱的愈合。方法36只成年雄性Wistar Albino大鼠（300 ~ 450 g）随机分为3组。建立大鼠右后腿全层股四头肌损伤模型。第一组不进行任何干预（对照组）。第二组在损伤后进行一期修复（PS组）。第三组进行一期修复，修复部位注射50 mg壳聚糖（PS+壳聚糖组）。于第0、3、7、14天采集大鼠尾静脉血。第14、28天，每组处死4例，进行组织病理学评估。第28天，每组取4只动物进行生物力学研究。结果PS+壳聚糖组第28天的平均总数值低于对照组第14、28天的平均总数值（P = 0.004）。第14天，PS组IL-1β平均值高于其他各组(P <；0.001)。PS+壳聚糖组第14天TGF-β均值较其他组低(P <；0.001)。监测组平均最大拉伸阻力为1.51 N/mm2。虽然没有发现具有统计学意义的结果，但PS+壳聚糖组表现出与完整肌腱最相似的生物力学值，而对照组表现出最不同的结果。结论壳聚糖可促进大鼠股四头肌肌腱损伤修复后的肌腱愈合。在我们的研究中，壳聚糖影响不同的途径并促进肌腱愈合。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

The role of chitosan in tissue healing after primary repair of quadriceps tendon ruptures: Experimental animal model

Aim

To expedite the healing of tendons by introducing chitosan to the repaired tendon site.

Methods

Thirty-six adult, male Wistar Albino rats (300–450 g) were randomly divided into three groups. A full-thickness quadriceps injury model was created in the right hind leg of all animals. The first group did not undergo any intervention (control group). In the second group, primary repair was performed following injury (primary suturing (PS) group). In the third group, primary repair was performed, and 50 mg chitosan was administered via injection at the repair site (PS+Chitosan group). Tail venous blood samples were collected from all rats on days 0, 3, 7 and 14. On days 14 and 28, four subjects from each group were sacrificed for histopathologic evaluation. On day 28, four subjects from each group were sacrificed for biomechanical investigation.

Results

The mean total value of the PS+Chitosan group on day 28 was lower than the mean total value of the control group on days 14 and 28 (P = 0.004). The mean IL-1β value on day 14 was higher in the PS group compared with the other groups (P < 0.001). The mean TGF-β value on day 14 was lower in the PS+Chitosan group compared with the other groups (P < 0.001). The average maximal tensile resistance in the monitor group was 1.51 N/mm². Although statistically significant results were not found, the PS+Chitosan group exhibited biomechanical values that were most similar to those of the intact tendon, while the control group displayed the most divergent results.

Conclusion

Chitosan application accelerates tendon healing after repair in quadriceps tendon injuries in our rat model. Chitosan affects different pathways and enhances tendon healing as observed in our study.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Knee 医学-外科

CiteScore

3.80

自引率

5.30%

发文量

171

审稿时长

6 months

期刊介绍： The Knee is an international journal publishing studies on the clinical treatment and fundamental biomechanical characteristics of this joint. The aim of the journal is to provide a vehicle relevant to surgeons, biomedical engineers, imaging specialists, materials scientists, rehabilitation personnel and all those with an interest in the knee. The topics covered include, but are not limited to: • Anatomy, physiology, morphology and biochemistry; • Biomechanical studies; • Advances in the development of prosthetic, orthotic and augmentation devices; • Imaging and diagnostic techniques; • Pathology; • Trauma; • Surgery; • Rehabilitation.