靶向转移性黑色素瘤：南瓜籽提取物作为潜在的抗肿瘤药物

IF 4.4 3区工程技术 Q2 CHEMISTRY, PHYSICAL

Journal of Supercritical Fluids Pub Date : 2025-04-29 DOI:10.1016/j.supflu.2025.106643

Bruno Antônio Amarante , Eduardo Saccomori , Denise Bilibio , João Paulo Bender , Gilnei Bruno da Silva , Daiane Manica , Margarete Dulce Bagatini , Alessandra Nejar Bruno , Wagner Luiz Priamo

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引用次数: 0

摘要

本研究研究了超声辅助提取（UAE）和加压乙醇提取（PEE）两种方法提取的南瓜籽提取物对黑色素瘤细胞的脂肪酸组成和抗肿瘤作用。采用实验设计对两种方法的参数进行优化，并以脂肪酸为基础进行气相色谱鉴定。用UAE和PEE提取物处理SK-MEL-28和A375黑色素瘤细胞，并评估其活力（MTT测定、吖啶橙染色）、氧化还原谱（NOx、PSH、NPSH）和线粒体膜电位（MMP）。UAE和PEE的提取率分别为48.99 %和30.28 %。主要脂肪酸包括亚油酸甲酯（~ 47 %）和顺式-9油酸甲酯（~ 25 %）。提取物降低了黑色素瘤细胞活力，增加了NOx，降低了MMP，特别是来自阿联酋的提取物，突出了提取方法的重要性。这些影响可能与氧化还原平衡改变、MMP减少和肿瘤细胞死亡有关。

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Targeting metastatic melanoma: Pumpkin seed extracts as potential antineoplastic agents

In this study, the fatty acid composition and antitumor potential of pumpkin seed extracts, obtained through ultrasound-assisted extraction (UAE) and pressurized ethanol extraction (PEE) against melanoma cells was studied. An experimental design was employed to optimize parameters in both methods and gas chromatography identified the extracts based on fatty acids. SK-MEL-28 and A375 melanoma cells were treated with UAE and PEE extracts and assessed for viability (MTT assay, acridine orange staining), redox profile (NOx, PSH, NPSH), and mitochondrial membrane potential (MMP). The highest extraction yields were 48.99 % for UAE and 30.28 % for PEE. Major fatty acids included methyl linoleate (∼ 47 %) and methyl cis-9 oleate (∼ 25 %). Extracts reduced melanoma cell viability, increased NOx, and decreased MMP, particularly with UAE-derived extracts, highlighting the significance of the extraction method. These effects may be linked to altered redox balance, reduced MMP, and tumor cell death.

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来源期刊

Journal of Supercritical Fluids 工程技术-工程：化工

CiteScore

7.60

自引率

10.30%

发文量

236

审稿时长

56 days

期刊介绍： The Journal of Supercritical Fluids is an international journal devoted to the fundamental and applied aspects of supercritical fluids and processes. Its aim is to provide a focused platform for academic and industrial researchers to report their findings and to have ready access to the advances in this rapidly growing field. Its coverage is multidisciplinary and includes both basic and applied topics. Thermodynamics and phase equilibria, reaction kinetics and rate processes, thermal and transport properties, and all topics related to processing such as separations (extraction, fractionation, purification, chromatography) nucleation and impregnation are within the scope. Accounts of specific engineering applications such as those encountered in food, fuel, natural products, minerals, pharmaceuticals and polymer industries are included. Topics related to high pressure equipment design, analytical techniques, sensors, and process control methodologies are also within the scope of the journal.