基于肠道菌群、代谢组学和转录组学的生地黄多糖对lps诱导小鼠免疫激活的影响

IF 8.5 1区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

International Journal of Biological Macromolecules Pub Date : 2025-05-07 DOI:10.1016/j.ijbiomac.2025.143981

Wensen Zhang , Na Cui , Fazhi Su , Meng Liu , Biao Li , Yanping Sun , Yuanning Zeng , Bingyou Yang , Haixue Kuang , Qiuhong Wang

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引用次数: 0

摘要

本研究探讨生地黄多糖（RP）对lps诱导的免疫激活的调节作用。RP是一种富含半乳糖和葡萄糖的杂多糖（6.34 kDa和4.63 kDa），以25 mg/kg和50 mg/kg的剂量给药于lps攻击的BALB/c小鼠。结果显示，RP可显著降低促炎因子（TNF-α、IL-6），降低氧化应激（MDA），提高抗氧化酶（SOD、GSH-Px）。恢复脾结构，减轻细胞凋亡，抑制TNF-α/NF-κB/IL-6通路。代谢组学将RP与鞘脂代谢联系起来，而肠道微生物群分析显示有益细菌增加和scfa升高。转录组学证实RP通过TNF信号进行免疫调节。这些发现表明RP通过调节炎症、肠道微生物群和SCFA产生来减轻免疫过度激活的潜力，表明免疫相关疾病的治疗前景。

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Effects of Rehmanniae Radix Praeparata polysaccharides on LPS-induced immune activation in mice based on gut microbiota, metabolomics and transcriptomics

This study explored the immunomodulatory effects of Rehmanniae Radix Praeparata polysaccharides (RP) on LPS-induced immune activation. RP, characterized as a heteropolysaccharide (6.34 kDa and 4.63 kDa) rich in galactose and glucose, was administered to LPS-challenged BALB/c mice at 25 mg/kg and 50 mg/kg doses. Results showed RP significantly reduced pro-inflammatory cytokines (TNF-α, IL-6), lowered oxidative stress (MDA), and boosted antioxidant enzymes (SOD, GSH-Px). It restored splenic structure, mitigated apoptosis, and suppressed the TNF-α/NF-κB/IL-6 pathway. Metabolomics linked RP to sphingolipid metabolism, while gut microbiota analysis revealed increased beneficial bacteria and elevated SCFAs. Transcriptomics confirmed RP's immune regulation via TNF signaling. These findings demonstrate RP's potential in alleviating immune overactivation by modulating inflammation, gut microbiota, and SCFA production, suggesting therapeutic promise for immune-related diseases.

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来源期刊

International Journal of Biological Macromolecules 生物-生化与分子生物学

CiteScore

13.70

自引率

9.80%

发文量

2728

审稿时长

64 days

期刊介绍： The International Journal of Biological Macromolecules is a well-established international journal dedicated to research on the chemical and biological aspects of natural macromolecules. Focusing on proteins, macromolecular carbohydrates, glycoproteins, proteoglycans, lignins, biological poly-acids, and nucleic acids, the journal presents the latest findings in molecular structure, properties, biological activities, interactions, modifications, and functional properties. Papers must offer new and novel insights, encompassing related model systems, structural conformational studies, theoretical developments, and analytical techniques. Each paper is required to primarily focus on at least one named biological macromolecule, reflected in the title, abstract, and text.