{Silk@Hyaluronic-Acid}杂种:抗DPPH的高效抗氧化剂和抗OH的弹性

Annita Theofanous, Yiannis Deligiannakis, Maria Louloudi
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引用次数: 0

摘要

抗自由基、抗氧化剂在健康、材料和技术应用方面具有重要的意义。通常,DPPH是评价抗氧化材料和技术时常用的自由基探针。羟基自由基(●OH)也无所不在;然而,评估它们对DPPH●抗氧化剂的普遍作用仍然具有挑战性。在本研究中,我们引入了具有增强耐久性的新型杂化抗氧化材料作为DPPH●清除剂,表现出对●OH的高抗性。将两种透明质酸单体(d -葡萄糖醛酸[GLA]和n -乙酰- d -氨基葡萄糖[GLAM])固定在脱胶蚕丝纤维表面,合成了杂化抗氧化剂。透明质酸是广泛应用于化妆品和医疗领域的重要产品,以其生物化学和治疗特性而闻名。丝绸,素有“纺织品女王”之称,具有非凡的结构和机械特性。通过对DPPH●自由基的反应来评价杂化材料的氢原子转移抗氧化活性。GLA@SFd@GLAM表现出最高的性能,每克材料可有效清除11 μmol的DPPH自由基。所有三种杂化材料都证明了可重复使用,在多个循环中保持其清除DPPH自由基的功效。利用电子顺磁共振光谱原位评价了杂化物对羟基自由基(●OH)的弹性。材料SFd@GLA, SFd@GLAM和GLA@SFd@GLAM在暴露于●OH自由基长达两个连续循环使用后仍保持其dpph抗氧化活性。我们讨论了{Silk@Hyaluronic-Acid}杂化物与DPPH●和●OH自由基相互作用的物理化学基础和机理。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

{Silk@Hyaluronic-Acid} Hybrids: Efficient Antioxidants Against DPPH● With Resilience Against ●OH

{Silk@Hyaluronic-Acid} Hybrids: Efficient Antioxidants Against DPPH● With Resilience Against ●OH

Antiradical, antioxidant agents are of great importance in health, materials, and technology applications. Usually, DPPH are the radicals typically used as probes in the evaluation of antioxidant materials and technologies. Hydroxyl radicals (OH) are also omnipresent; however, assessment of their pervasive role on DPPH-antioxidants remains challenging. In this study, we introduce novel hybrid antioxidant materials with enhanced durability as DPPH-scavengers, demonstrating high resistance to OH. The hybrid antioxidants were synthesized by immobilizing the two monomers of hyaluronic acid (D-Glucuronic Acid [GLA], and N-Acetyl-D-Glucosamine [GLAM]) onto the surface of degummed silk fibers. Hyaluronic acid, a prominent product widely utilized in cosmetics and medical applications, is renowned for its biochemical and therapeutic properties. Silk, commonly known as the “queen of textiles,” possesses remarkable structural and mechanical attributes. The hybrid materials' hydrogen atom transfer antioxidant activity was evaluated through their reactivity toward DPPH radicals. GLA@SFd@GLAM, exhibited the highest performance, effectively scavenging a total amount of 11 μmol of DPPH radicals per gram of material. All three hybrid materials demonstrated reusability, maintaining their efficacy in scavenging DPPH radicals over multiple cycles. The resilience of the hybrids, against hydroxyl radicals (OH), was evaluated in-situ using Electron Paramagnetic Resonance spectroscopy. The materials SFd@GLA, SFd@GLAM, and GLA@SFd@GLAM retained their DPPH-antioxidant activity after exposure to OH radicals for up to two consecutive cycles of use. We discuss the physicochemical basis and mechanisms of the interactions of the {Silk@Hyaluronic-Acid} hybrids with DPPH and OH radicals.

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