四种Lamiaceae药用植物的药理筛选，其中两种是台湾特有的：凋亡和细胞毒性特性

IF 2.2 4区医学 Q2 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Herbal Medicine Pub Date : 2025-04-23 DOI:10.1016/j.hermed.2025.101020

Arzu KASKA , Mücahit SEÇME

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引用次数: 0

摘要

lamiaceae植物由于其化学成分而具有抗癌、抗氧化和抗菌活性。研究了四种Lamiaceae药用植物（thyymus zygioides, Teucrium sandrasicum, Origanum sipyleum, Nepeta italica subsp.）的水乙醇提取物的药理作用。MDA-MB-231乳腺癌细胞株对凋亡和细胞周期的影响。方法采用XTT法测定这些Lamiaceae药用植物水乙醇提取物对MDA-MB-231细胞株细胞活力的细胞毒作用。实时荧光定量PCR检测细胞凋亡和细胞周期检查点调节因子Bax、Bcl-2、H19、RNU43、caspase-3、caspase-8、caspase-9、cyclin D1、cdk4、cdk6的mRNA表达变化。结果四种Lamiaceae药用植物水乙醇提取物对MDA-MB-231乳腺癌细胞均表现出较强的抗增殖活性，且呈时间依赖性和浓度依赖性。结论这些Lamiaceae药用植物作为抗增殖药物的潜在应用是了解植物在 rkiye中的药用价值的重要一步。

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Pharmacological Screening of Four Medicinal Lamiaceae Plants, Two of Which Are Endemic to Türkiye: Apoptotic and Cytotoxic Characteristics

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Pharmacological Screening of Four Medicinal Lamiaceae Plants, Two of Which Are Endemic to Türkiye: Apoptotic and Cytotoxic Characteristics

Introduction

Lamiaceae plants possess anticancer, antioxidant, and antimicrobial activities due to their phytochemical constituents. The aim of this study is to investigate the effects of the hydroethanolic extracts of four medicinal Lamiaceae plants (Thymus zygioides, Teucrium sandrasicum, Origanum sipyleum, Nepeta italica subsp. cadmea) to apoptosis and cell cycle on MDA-MB-231 breast cancer cell line.

Methods

The cytotoxic effects of hydroethanolic extracts of these medicinal Lamiaceae plants on cell viability in the MDA-MB-231 cell line were determined by XTT assay. mRNA expression changes of apoptosis and cell-cycle checkpoint regulators (Bax, Bcl-2, H19, RNU43, caspase-3, caspase-8, caspase-9, cyclin D1, CDK 4, CDK 6) were evaluated by real-time PCR.

Results

The hydroethanolic extracts from four medicinal Lamiaceae plants have exhibited high antiproliferative activity in MDA-MB-231 breast cancer cells according to time and concentration-dependent manner.

Conclusions

The potential application of these Lamiaceae medicinal plants as an antiproliferative agent is an important step in understanding the medicinal use of plants in Türkiye.

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来源期刊

Journal of Herbal Medicine INTEGRATIVE & COMPLEMENTARY MEDICINE-

CiteScore

3.90

自引率

0.00%

发文量

期刊介绍： The Journal of Herbal Medicine, the official journal of the National Institute of Medical Herbalists, is a peer reviewed journal which aims to serve its readers as an authoritative resource on the profession and practice of herbal medicine. The content areas of the journal reflect the interests of Medical Herbalists and other health professionals interested in the clinical and professional application of botanical medicines. The objective is to strengthen the research and educational base of herbal medicine with research papers in the form of case studies, original research articles and reviews, monographs, clinical trials and relevant in vitro studies. It also publishes policy statements, opinion pieces, book reviews, conference proceedings and profession related information such as pharmacovigilance reports providing an information source for not only the Herbal Practitioner but any Health professional with an interest in phytotherapy.