CsRAP2-7通过直接激活CsACO1负向调控柑橘表皮蜡合成和抗旱性

IF 6.1 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry Pub Date : 2025-05-05 DOI:10.1016/j.plaphy.2025.109983

Xinyue Zhao, Jingheng Xie, Li Yang, Wei Hu, Jie Song, Liuqing Kuang, Yingjie Huang, Yong Liu, Dechun Liu

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引用次数: 0

摘要

角质层蜡质在提高植物抗逆性方面起着重要作用。虽然角质层蜡生物合成的正调节因子研究得很好，但在柑橘中，负调节因子仍未被广泛探索。本文从脐橙中筛选并克隆了AP2/ERF家族基因CsRAP2-7。该基因定位于细胞核，受干旱和ABA处理的诱导。柠檬中过量表达CsRAP2-7可上调乙烯生物合成，同时抑制角质层蜡积累，降低角质层通透性，共同导致耐旱性显著下降。CsRAP2-7通过直接结合乙烯生物合成基因CsACO1的启动子，激活其转录，从而介导其调控作用。综上所述，CsRAP2-7通过直接介导CsACO1的表达，在角质层蜡质生物合成和抗旱性调控中发挥负向作用。

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CsRAP2-7 negatively regulates cuticular wax biosynthesis and drought resistance in citrus by directly activating CsACO1

Cuticular wax plays an important role in enhancing plant stress tolerance. While positive regulators of cuticular wax biosynthesis are well-studied, negative regulators remain largely unexplored in citrus. In the present paper, we screened and cloned an AP2/ERF family gene, CsRAP2-7, from navel orange. This gene is localized to the nucleus and induced by drought and ABA treatments. Overexpression of CsRAP2-7 in lemon upregulates ethylene biosynthesis while concurrently inhibiting cuticular wax accumulation and reducing cuticular permeability, collectively leading to a marked decline in drought tolerance. CsRAP2-7 mediates its regulatory role by directly binding to the promoter of CsACO1, an ethylene biosynthetic gene, thereby activating its transcription. These results suggest that CsRAP2-7 play a negative role in regulating cuticular wax biosynthesis and drought resistance by directly mediating CsACO1 expression.

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来源期刊

Plant Physiology and Biochemistry 生物-植物科学

CiteScore

11.10

自引率

3.10%

发文量

410

审稿时长

33 days

期刊介绍： Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement. Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB. Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.