Preparation of AuPt@ZIF-67 nanomaterials and their application in flow injection chemiluminescence immunoassay

Compared to natural enzymes, which are prone to deactivation, require expensive purification processes, and have stringent storage demands, nanozymes demonstrate superior stability, cost-efficiency, and customizable catalytic performance. In this study, the AuPt@ZIF-67 nano-enzyme composites with high catalytic activity were constructed by loading Au and Pt nanoparticles onto the zeolitic imidazolate framework-67 (ZIF-67). Capitalizing on the porous structure of ZIF-67 and the electronic synergy between the Au-Pt bimetallic components, the material demonstrated exceptional peroxidase-like activity. Harnessing the engineered properties of this advanced nanomaterial, a flow-injection chemiluminescence immunoassay (FI-CLIA) platform was engineered to achieve ultrarapid and ultrasensitive monitoring of aflatoxin B1 (AFB1) in foodstuffs. Experimental results indicated a linear dynamic range of 0.002~1000 ng mL-1 for AFB1 quantification, achieving an ultralow detection limit of 0.68 pg mL-1 (S/N = 3). The spiked recovery rates for corn and wheat samples ranged from 81.5% to 110.3%. The findings indicated that this method exhibits significant applicability prospects for AFB1 detection in actual sample analysis.