声流控生物组装诱导的治疗性组织制造的形态发生

IF 15.7 1区 综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES
Byungjun Kang, Eunseon Jeong, Seung Yeop Han, Jeong Hyun Heo, Yunam Lee, Suah Choi, Yunjung Choi, Donyoung Kang, Youn-Hoo Hwang, Jiin Lee, Jung Hwa Seo, Jinyoung Kim, Inhea Jeong, Enji Kim, Juyoung Lee, Dae-Eun Kim, Jang-Ung Park, Sung-Rae Cho, Yoonhee Jin, Seung-Woo Cho, Hyungsuk Lee
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引用次数: 0

摘要

为了构建用于治疗的体外组织,必须复制体内形态发生过程中细胞的空间分布。然而,在组织制造过程中同时调节细胞的几何排列和聚集仍然是技术上的挑战。在这里,我们介绍了声流控生物组装诱导的形态发生,它是由声流控线索产生的机械力对细胞的精确排列,以及细胞在体外和体内培养中形态和功能变化的结合。声流控生物组件可用于创建具有调节的纳米、微观和宏观结构的组织。我们证明,用声流体生物组件制造的神经肌肉组织表现出增强的收缩动力学、电生理和治疗效果。通过在活体组织的缺陷部位制造人工组织,证明了声流控生物组件作为原位应用的潜力。声流控生物组装诱导的形态发生为生物医学组织的制备提供了一个开创性的平台。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Acoustofluidic bioassembly induced morphogenesis for therapeutic tissue fabrication

Acoustofluidic bioassembly induced morphogenesis for therapeutic tissue fabrication

To build in vitro tissues for therapeutic applications, it is essential to replicate the spatial distribution of cells that occurs during morphogenesis in vivo. However, it remains technically challenging to simultaneously regulate the geometric alignment and aggregation of cells during tissue fabrication. Here, we introduce the acoustofluidic bioassembly induced morphogenesis, which is the combination of precise arrangement of cells by the mechanical forces produced by acoustofluidic cues, and the morphological and functional changes of cells in the following in vitro and in vivo cultures. The acoustofluidic bioassembly can be used to create tissues with regulated nano-, micro-, and macro-structures. We demonstrate that the neuromuscular tissue fabricated with the acoustofluidic bioassembly exhibits enhanced contraction dynamics, electrophysiology, and therapeutic efficacy. The potential of the acoustofluidic bioassembly as an in situ application is demonstrated by fabricating artificial tissues at the defect sites of living tissues. The acoustofluidic bioassembly induced morphogenesis can provide a pioneering platform to fabricate tissues for biomedical applications.

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来源期刊
Nature Communications
Nature Communications Biological Science Disciplines-
CiteScore
24.90
自引率
2.40%
发文量
6928
审稿时长
3.7 months
期刊介绍: Nature Communications, an open-access journal, publishes high-quality research spanning all areas of the natural sciences. Papers featured in the journal showcase significant advances relevant to specialists in each respective field. With a 2-year impact factor of 16.6 (2022) and a median time of 8 days from submission to the first editorial decision, Nature Communications is committed to rapid dissemination of research findings. As a multidisciplinary journal, it welcomes contributions from biological, health, physical, chemical, Earth, social, mathematical, applied, and engineering sciences, aiming to highlight important breakthroughs within each domain.
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