基于真皮基质的生物活性和可生物降解的水凝胶负载含有血管内皮生长因子(VEGF)和白细胞介素(IL)-10的微球,加速了大鼠糖尿病伤口的愈合

IF 3.4 3区 环境科学与生态学 Q3 CELL & TISSUE ENGINEERING
Zihan Wang , Jia Liu , Runpei Deng , Yang Yang , Chen Fangfang , Shi Yonghui
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引用次数: 0

摘要

糖尿病性伤口是糖尿病患者最常见和最昂贵的并发症之一。因此,已经进行了大量的研究来探索加速伤口愈合过程的有效方法。与其他材料相比,生物水凝胶具有良好的性能,因此经常用于伤口愈合。在这项研究中,我们评估了一种生物活性和可降解的基于真皮基质(HDM)的水凝胶包被含有血管内皮生长因子(VEGF)和白细胞介素(IL)-10的微球对糖尿病大鼠伤口愈合的影响。将45只糖尿病大鼠随机分为3组(n = 15):对照组、HDM组和含有VEGF和IL-10 (HDMM)微球的HDM组。另取非糖尿病未治疗大鼠(健康组)作为对照(n = 15)。在第7、14和21天进行伤口评估。结果显示,与对照组相比,治疗组在伤口愈合率、成纤维细胞和血管计数、胶原密度、TGF-β和VEGF细胞因子浓度水平以及生物力学参数方面均有显著改善,其中以HDMM组效果最显著。此外,与其他组相比,HDMM组表现出更大的中性粒细胞和巨噬细胞计数减少,以及TNF-α和IL-1β细胞因子水平下降。综上所述,负载含有VEGF和IL-10的微球的HDM在促进糖尿病创面愈合方面的作用更为显著。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Bioactive and biodegradable hydrogel based on the dermal matrix loaded with microspheres containing vascular endothelial growth factor (VEGF) and interleukin (IL)-10 accelerated the healing of diabetic wounds in rats
Diabetic wounds are among the most common and costly complications in diabetic patients. As a result, numerous studies have been conducted to explore effective approaches for accelerating the wound healing process. Biological hydrogels are frequently used for wound healing due to their favorable properties compared to other materials. In this study, we evaluated the effect of a bioactive and degradable hydrogel based on dermal matrix (HDM) encapsulated with microspheres containing vascular endothelial growth factor (VEGF) and interleukin (IL)-10 on promoting wound healing in diabetic rats. Forty-five diabetic rats were randomly assigned to three groups (n = 15): control, HDM, and HDM encapsulated with microspheres containing VEGF and IL-10 (HDMM). In addition, non-diabetic untreated rats (healthy group) were considered as control (n = 15). Wound assessments were performed on days 7, 14, and 21. The results demonstrated significant improvements in wound closure rate, fibroblast and blood vessel counts, collagen density, concentration levels of TGF-β and VEGF cytokines, and biomechanical parameters in the treatment groups compared to the control group, with the most pronounced effects observed in the HDMM group. Additionally, the HDMM group exhibited a greater reduction in neutrophil and macrophage counts, as well as decreased levels of TNF-α and IL-1β cytokines compared to the other groups. In conclusion, the HDM loaded with microspheres containing VEGF and IL-10 showed a more significant effect in promoting the healing of diabetic wounds.
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来源期刊
Regenerative Therapy
Regenerative Therapy Engineering-Biomedical Engineering
CiteScore
6.00
自引率
2.30%
发文量
106
审稿时长
49 days
期刊介绍: Regenerative Therapy is the official peer-reviewed online journal of the Japanese Society for Regenerative Medicine. Regenerative Therapy is a multidisciplinary journal that publishes original articles and reviews of basic research, clinical translation, industrial development, and regulatory issues focusing on stem cell biology, tissue engineering, and regenerative medicine.
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