评估原发性开角型青光眼和伪去角质型青光眼严重程度的细胞因子分析

Q3 Medicine

Archivos De La Sociedad Espanola De Oftalmologia Pub Date : 2025-05-01 DOI:10.1016/j.oftal.2024.12.007

L. Gallardo Martin , B. Burgos-Blasco , B. Vidal-Villegas , J.I. Fernandez-Vigo , J. Garcia-Feijoo , J.M. Martinez-de-la-Casa

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引用次数: 0

摘要

目的分析泪液和体液中细胞因子作为青光眼严重程度评价的生物标志物。DesignCross-sectional研究。研究对象和方法从15例剥脱性青光眼（XFG）患者、43例原发性开角型青光眼（POAG）患者和33例符合白内障手术条件的对照患者中收集肝液和房水样本。采用Bio-Plex Pro人细胞因子27- plex免疫分析试剂盒，对泪液和房水样品中27种炎性细胞因子进行分析。白细胞介素(IL)-1b、IL-1ra、IL-2、IL-4、IL-5、IL-6、IL-7、IL-8、IL-9、IL-10、IL-12、IL-13、IL-15、IL-17、eotaxin、成纤维细胞生长因子（FGF）碱性、粒细胞集落刺激因子、粒细胞-单核细胞集落刺激因子、干扰素-γ、干扰素-γ诱导蛋白、单核细胞化学引诱蛋白-1、巨噬细胞炎症蛋白(MIP)-1a、MIP-1b、血小板源性生长因子，通过活化正常T细胞表达和分泌而调节；肿瘤坏死因子-α和血管内皮生长因子。结果各组泪液中IL-2、IL-4、IL-9、IL-12、IL-15及FGF-basic含量差异均有统计学意义。房水中，IL-5、IL-6、IL-12、MCP-1等细胞因子的表达差异有统计学意义。IL-4和IL-15的差异与外用药物的数量有关，IL-9和IL-15的差异与视野平均缺损有关。结论炎症与青光眼密切相关，部分细胞因子可能与青光眼的严重程度有关。

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Análisis de citocinas para evaluar la gravedad en el glaucoma primario de ángulo abierto y glaucoma pseudoexfoliativo

Purpose

To analyze cytokines in tear and aqueous humour as biomarkers for evaluation of severity of glaucoma.

Design

Cross-sectional study.

Subjects and methods

Tear and aqueous humour samples were collected from 15 exfoliative glaucoma (XFG) patients, 43 primary open-angle glaucoma (POAG) patients and 33 controls (eligible for cataract surgery).

A total of 27 inflammatory cytokines were analyzed in the tear and aqueous humor samples by the Bio-Plex Pro Human Cytokine 27-Plex Immunoassay kit: interleukin (IL)-1b, IL-1ra, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12, IL-13, IL-15, IL-17, eotaxin, fibroblast growth factor (FGF) basic, granulocyte colony-stimulating factor, granulocyte-monocyte colony-stimulating factor, interferon-γ, interferon gamma-induced protein, monocyte chemo-attractant protein-1, macrophage inflammatory protein (MIP)-1a, MIP-1b, platelet-derived growth factor, regulated on activation normal T cell expressed and secreted, tumor necrosis factor-α and vascular endothelial growth factor.

Results

In the tear samples a statistically significant difference in IL-2, IL-4, IL-9, IL-12, IL-15 and FGF-basic between study groups was observed. In aqueous humor, a statistically significant difference of the following cytokines was observed: IL-5, IL-6, IL-12 and MCP-1. IL-4 and IL-15 differences correlate with the number of topical drugs, while IL-9 and IL-15 are associated with the visual field mean defect.

Conclusion

Inflammation is strongly associated with glaucoma and some cytokines may be related to glaucoma severity.

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来源期刊

Archivos De La Sociedad Espanola De Oftalmologia Medicine-Ophthalmology

CiteScore

1.20

自引率

0.00%

发文量

109

审稿时长

78 days

期刊介绍： La revista Archivos de la Sociedad Española de Oftalmología, editada mensualmente por la propia Sociedad, tiene como objetivo publicar trabajos de investigación básica y clínica como artículos originales; casos clínicos, innovaciones técnicas y correlaciones clinicopatológicas en forma de comunicaciones cortas; editoriales; revisiones; cartas al editor; comentarios de libros; información de eventos; noticias personales y anuncios comerciales, así como trabajos de temas históricos y motivos inconográficos relacionados con la Oftalmología. El título abreviado es Arch Soc Esp Oftalmol, y debe ser utilizado en bibliografías, notas a pie de página y referencias bibliográficas.