高效转化葡萄糖到氢的基因工程混合生物系统

IF 7.3 1区 化学 Q1 CHEMISTRY, MULTIDISCIPLINARY
Zhengyu Tao, Song Lin, Shangsong Li, Baoyuan Li, Rui Nie and Xin Huang*, 
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引用次数: 0

摘要

半导体纳米颗粒和细菌的整合提出了一种诱人的方法,通过利用太阳能作为额外的驱动力来增加发酵制氢。然而,在生物混合系统中有效利用和转化基质方面仍有很大的改进空间。本研究通过多基因敲除hycA、ldhA和frdD,构建了一种新型工程大肠杆菌(E. coli)菌株,以增强丙酮酸生成甲酸的通量。随后,在镉源和硒源存在的情况下,通过细胞内生物矿化,CdSexS1-x纳米颗粒可以在工程菌株内部特殊形成,从而实现葡萄糖到氢的高转化效率,高达1.86 mol·H2·mol - 1·G-1(葡萄糖到H2理论值的93%),是目前生物杂交体系中转化率最高的。因此,预计该研究将为突破生物制氢效率瓶颈提供一条有希望的途径。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Genetically Engineered Hybrid Biosystem for Highly Efficient Conversion from Glucose to Hydrogen

Genetically Engineered Hybrid Biosystem for Highly Efficient Conversion from Glucose to Hydrogen

The integration of semiconductor nanoparticles and bacteria presents an enticing method to augment fermentative hydrogen production by leveraging solar energy as an additional driving force. However, there remains significant room for improvement in effectively using and transforming substrates within the biohybrid system. Here, a new type of engineered Escherichia coli (E. coli) strain is constructed by multigene knockout of hycA, ldhA, and frdD to strengthen the flux of pyruvate to formate. Subsequently, upon intracellular biomineralization in the presence of a cadmium source and a selenium source, CdSexS1–x nanoparticles could be specially formed inside the engineered strain, and then a high conversion efficiency from glucose to hydrogen is achieved, as high as 1.86 mol·H2·mol–1·G–1 (93% of the theoretical value from glucose to H2), which currently boasts the highest conversion ratio among biohybrid systems. Therefore, it is anticipated that such a study could contribute a promising way to break through the bottleneck of biological hydrogen production efficiency.

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来源期刊
ACS Sustainable Chemistry & Engineering
ACS Sustainable Chemistry & Engineering CHEMISTRY, MULTIDISCIPLINARY-ENGINEERING, CHEMICAL
CiteScore
13.80
自引率
4.80%
发文量
1470
审稿时长
1.7 months
期刊介绍: ACS Sustainable Chemistry & Engineering is a prestigious weekly peer-reviewed scientific journal published by the American Chemical Society. Dedicated to advancing the principles of green chemistry and green engineering, it covers a wide array of research topics including green chemistry, green engineering, biomass, alternative energy, and life cycle assessment. The journal welcomes submissions in various formats, including Letters, Articles, Features, and Perspectives (Reviews), that address the challenges of sustainability in the chemical enterprise and contribute to the advancement of sustainable practices. Join us in shaping the future of sustainable chemistry and engineering.
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