Zhengyu Tao, Song Lin, Shangsong Li, Baoyuan Li, Rui Nie and Xin Huang*,
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Genetically Engineered Hybrid Biosystem for Highly Efficient Conversion from Glucose to Hydrogen
The integration of semiconductor nanoparticles and bacteria presents an enticing method to augment fermentative hydrogen production by leveraging solar energy as an additional driving force. However, there remains significant room for improvement in effectively using and transforming substrates within the biohybrid system. Here, a new type of engineered Escherichia coli (E. coli) strain is constructed by multigene knockout of hycA, ldhA, and frdD to strengthen the flux of pyruvate to formate. Subsequently, upon intracellular biomineralization in the presence of a cadmium source and a selenium source, CdSexS1–x nanoparticles could be specially formed inside the engineered strain, and then a high conversion efficiency from glucose to hydrogen is achieved, as high as 1.86 mol·H2·mol–1·G–1 (93% of the theoretical value from glucose to H2), which currently boasts the highest conversion ratio among biohybrid systems. Therefore, it is anticipated that such a study could contribute a promising way to break through the bottleneck of biological hydrogen production efficiency.
期刊介绍:
ACS Sustainable Chemistry & Engineering is a prestigious weekly peer-reviewed scientific journal published by the American Chemical Society. Dedicated to advancing the principles of green chemistry and green engineering, it covers a wide array of research topics including green chemistry, green engineering, biomass, alternative energy, and life cycle assessment.
The journal welcomes submissions in various formats, including Letters, Articles, Features, and Perspectives (Reviews), that address the challenges of sustainability in the chemical enterprise and contribute to the advancement of sustainable practices. Join us in shaping the future of sustainable chemistry and engineering.