马来西亚、印度和中国稻瘟病菌AvrPi-54基因新单倍型的检测

IF 1 Q4 GENETICS & HEREDITY

Gene Reports Pub Date : 2025-05-01 DOI:10.1016/j.genrep.2025.102242

Elissa Stella Rafael , Freddy Kuok San Yeo , Walftor Dumin , Surisa Phornvillay , Jun Wah Yap , Najaa Batrisyia Abdullah Zakaria , Lee San Lai

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引用次数: 0

摘要

稻瘟病病原稻瘟菌（pyricaria oryzae）对水稻生产构成重大威胁。关于Avr基因的遗传多样性和沙捞越水稻对马来西亚稻瘟病分离株的抗性谱资料有限。本研究旨在通过与国家生物技术信息中心（National Centre for Biotechnology Information， NCBI）数据库中的AvrPi-54基因序列的比较，研究沙捞越稻稻谷P. oryzae分离株AvrPi-54基因的序列差异。分析了20株稻瘟病菌分离株和98个AvrPi-54基因序列。鉴定出9种不同的单倍型，包括6种新的单倍型。AvrPi-54基因处于正选择压力下。值得注意的是，沙捞越水稻对携带H1单倍型的稻瘟病菌株表现出不同程度的敏感性。了解AvrPi-54与其他Avr基因的遗传多样性，对于制定有效的策略来管理马来西亚沙捞越的米曲霉至关重要。

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New haplotypes of AvrPi-54 gene detected in Pyricularia oryzae from Malaysia, India and China

Pyricularia oryzae, the causal agent of rice blast disease, poses a significant threat to rice production. Limited information exists regarding the genetic diversity of Avr genes and the resistance profile of Sarawak rice against P. oryzae isolates in Malaysia. This study aimed to investigate the sequence variation of the AvrPi-54 gene in P. oryzae isolates from Sarawak rice, in comparison with sequences available in the National Centre for Biotechnology Information (NCBI) database. A total of 20 P. oryzae isolates were analyzed together with 98 AvrPi-54 gene sequences from NCBI. Nine distinct haplotypes were identified, including six novel haplotypes. The AvrPi-54 gene was found to be under positive selection pressure. Notably, Sarawak rice exhibited varying levels of susceptibility to P. oryzae isolates carrying the H1 haplotype. Understanding the genetic diversity of AvrPi-54 together with other Avr genes is crucial for developing effective strategies to manage P. oryzae in Sarawak, Malaysia.

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来源期刊

Gene Reports Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

3.30

自引率

7.70%

发文量

246

审稿时长

49 days

期刊介绍： Gene Reports publishes papers that focus on the regulation, expression, function and evolution of genes in all biological contexts, including all prokaryotic and eukaryotic organisms, as well as viruses. Gene Reports strives to be a very diverse journal and topics in all fields will be considered for publication. Although not limited to the following, some general topics include: DNA Organization, Replication & Evolution -Focus on genomic DNA (chromosomal organization, comparative genomics, DNA replication, DNA repair, mobile DNA, mitochondrial DNA, chloroplast DNA). Expression & Function - Focus on functional RNAs (microRNAs, tRNAs, rRNAs, mRNA splicing, alternative polyadenylation) Regulation - Focus on processes that mediate gene-read out (epigenetics, chromatin, histone code, transcription, translation, protein degradation). Cell Signaling - Focus on mechanisms that control information flow into the nucleus to control gene expression (kinase and phosphatase pathways controlled by extra-cellular ligands, Wnt, Notch, TGFbeta/BMPs, FGFs, IGFs etc.) Profiling of gene expression and genetic variation - Focus on high throughput approaches (e.g., DeepSeq, ChIP-Seq, Affymetrix microarrays, proteomics) that define gene regulatory circuitry, molecular pathways and protein/protein networks. Genetics - Focus on development in model organisms (e.g., mouse, frog, fruit fly, worm), human genetic variation, population genetics, as well as agricultural and veterinary genetics. Molecular Pathology & Regenerative Medicine - Focus on the deregulation of molecular processes in human diseases and mechanisms supporting regeneration of tissues through pluripotent or multipotent stem cells.