严格厌氧革兰氏阴性菌连翘单宁菌的红外光谱生化表征

IF 4.3 2区化学 Q1 SPECTROSCOPY

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy Pub Date : 2025-04-29 DOI:10.1016/j.saa.2025.126317

Geraldo Magno Alves de Abreu , Inglid Fontoura , Maiara Lima Castilho , Sônia Khouri Sibelino , Claudio Alberto Téllez Soto , Airton Abrahão Martin

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引用次数: 0

摘要

连珠单宁菌是一种挑剔的s层革兰氏阴性、严格厌氧和高度多形性细菌，与牙周炎症过程、骨质流失、心血管死亡率、系统性后遗症、败血症和早产有关。本研究的目的之一是首次利用傅里叶变换红外显微光谱技术对连翘单宁（ATCC 43037）进行表征。该分析旨在建立一种合适的方法来获得表征该微生物的可重复光谱，并使用傅立叶自反卷积分析（FSD）和二阶导数光谱以及高斯波段拟合等技术来分配振动带。该研究进行了三个重复，每个培养共产生25个光谱，跨重复产生75个光谱。该方法可用于连翘单宁的生物化学鉴定和鉴定。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Biochemical characterization of the strict anaerobic Gram-negative bacteria Tannerella forsythia by infrared micro-spectroscopy

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Biochemical characterization of the strict anaerobic Gram-negative bacteria Tannerella forsythia by infrared micro-spectroscopy

Tannerella forsythia is a fastidious S-layer Gram-negative, strict anaerobic, and highly pleomorphic bacterium associated with periodontal inflammatory processes, bone loss, cardiovascular mortality, systemic sequelae, sepsis, and preterm birth. One of the purposes of this study was to characterize, for the first time, Tannerella forsythia (ATCC 43037) using Fourier transform infrared micro-spectroscopy. The analysis aimed to establish a suitable methodology for obtaining reproducible spectra for the characterization of this microorganism, as well as to assign the vibrational bands using techniques such Fourier self-deconvolution analysis (FSD) and second derivative spectral followed by Gaussian band fitting. The study was carried out in triplicate, yielding a total of 25 spectra per culture and 75 spectra across replicates. This methodology can be applied to the biochemical identification and characterization of Tannerella forsythia.

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来源期刊

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 物理-光谱学

CiteScore

8.40

自引率

11.40%

发文量

1364

审稿时长

40 days

期刊介绍： Spectrochimica Acta, Part A: Molecular and Biomolecular Spectroscopy (SAA) is an interdisciplinary journal which spans from basic to applied aspects of optical spectroscopy in chemistry, medicine, biology, and materials science. The journal publishes original scientific papers that feature high-quality spectroscopic data and analysis. From the broad range of optical spectroscopies, the emphasis is on electronic, vibrational or rotational spectra of molecules, rather than on spectroscopy based on magnetic moments. Criteria for publication in SAA are novelty, uniqueness, and outstanding quality. Routine applications of spectroscopic techniques and computational methods are not appropriate. Topics of particular interest of Spectrochimica Acta Part A include, but are not limited to: Spectroscopy and dynamics of bioanalytical, biomedical, environmental, and atmospheric sciences, Novel experimental techniques or instrumentation for molecular spectroscopy, Novel theoretical and computational methods, Novel applications in photochemistry and photobiology, Novel interpretational approaches as well as advances in data analysis based on electronic or vibrational spectroscopy.