环境相关浓度多菌灵单独或与二苯甲酮-3联合对非洲爪蟾睾丸发育的影响

IF 4.1 2区环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY

Aquatic Toxicology Pub Date : 2025-04-30 DOI:10.1016/j.aquatox.2025.107393

Jie Li , Hai-Ming Xu , Xing-Hong Li , Yuan-Yuan Li , Zhan-Fen Qin

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引用次数: 0

摘要

杀菌剂多菌灵（CBZ）广泛存在于水生环境中，据报道会破坏啮齿动物的睾丸发育，但其对两栖动物的影响尚不清楚。本实验研究了环境相关浓度CBZ（1、10、100 μg/L）对45/46 ~ 52期非洲爪蟾早期睾丸发育的影响。所有浓度CBZ均对蝌蚪发育有抑制作用，但浓度为10和100 μg/L的CBZ均引起睾丸发育迟缓，表现为形态异常、细胞增殖抑制和生殖细胞减少。同时在一定浓度组中，睾丸偏倚基因下调，卵巢偏倚基因上调。虽然1 μg/L CBZ并未导致这些睾丸参数发生显著变化，但其下降趋势值得关注，特别是考虑到其与其他污染物的联合暴露。因此，我们进行了实验二，以研究1 μg/L CBZ与紫外线过滤器二苯甲酮-3 （BP-3）联合作用对45/46期至66期睾丸发育的影响。正如预期的那样，我们发现，与对照组相比，1 μg/L CBZ和2.28 μg/L BP-3共同暴露使睾丸尺寸变小，生殖细胞减少，而单独暴露对这两个参数没有显著影响，显示出对睾丸发育的协同作用。此外，它们的联合暴露在变质延迟、肝脏重量增加和肝脏组织学改变方面也具有协同作用。我们的研究结果强调了环境相关浓度的CBZ单独或与紫外线过滤器结合对睾丸发育的影响，强调了这些污染物特别是它们的混合物对两栖动物的生态风险，这值得继续关注。

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Effects of environmentally relevant concentrations of carbendazim, either alone or combined with benzophenone-3, on testicular development in Xenopus laevis

The fungicide carbendazim (CBZ), widely present in aquatic environments, has been reported to disrupt testicular development in rodents, yet its impacts on amphibians remain poorly understood. Here, we investigated the effects of environmentally-relevant concentrations of CBZ (1, 10, 100 μg/L) on early testicular development in Xenopus laevis following exposure from Nieuwkoop-Faber (NF) stages 45/46 to 52 in Experiment I. While all the concentrations of CBZ inhibited tadpole development, 10 and 100 μg/L CBZ caused retarded testicular development, which was characterized by morphological abnormality, suppressed cell proliferation and reduced germ cells, along with downregulation of testis-biased genes and upregulation of ovary-biased genes in certain concentration groups. Although 1 μg/L CBZ did not lead to significant changes in these testicular parameters, their declining trends are of concern, especially when considering its combined exposure with other contaminants. Thus, we conducted Experiment II to address the combined effects of 1 μg/L CBZ with the UV filter benzophenone-3 (BP-3), which was reported to affect testicular development in X. laevis, on testicular development from stages 45/46 to 66. As expected, we found that co-exposure to 1 μg/L CBZ and 2.28 μg/L BP-3 produced smaller testis size accompanied with fewer germ cells compared to the control, while alone exposure had no significant effects on the two parameters, exhibiting a synergistic effect on testicular development. Besides, their combined exposure also exerted synergistic effects with regard to metamorphic delay, increased liver weight and hepatic histological changes. Our findings emphasize the impacts of environmentally relevant concentrations of CBZ alone or in combination with UV-filters on testicular development, highlighting the ecological risk of these contaminants especially their mixtures for amphibians, which warrants continued attention.

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来源期刊

Aquatic Toxicology 环境科学-毒理学

CiteScore

7.10

自引率

4.40%

发文量

250

审稿时长

56 days

期刊介绍： Aquatic Toxicology publishes significant contributions that increase the understanding of the impact of harmful substances (including natural and synthetic chemicals) on aquatic organisms and ecosystems. Aquatic Toxicology considers both laboratory and field studies with a focus on marine/ freshwater environments. We strive to attract high quality original scientific papers, critical reviews and expert opinion papers in the following areas: Effects of harmful substances on molecular, cellular, sub-organismal, organismal, population, community, and ecosystem level; Toxic Mechanisms; Genetic disturbances, transgenerational effects, behavioral and adaptive responses; Impacts of harmful substances on structure, function of and services provided by aquatic ecosystems; Mixture toxicity assessment; Statistical approaches to predict exposure to and hazards of contaminants The journal also considers manuscripts in other areas, such as the development of innovative concepts, approaches, and methodologies, which promote the wider application of toxicological datasets to the protection of aquatic environments and inform ecological risk assessments and decision making by relevant authorities.