环介导等温扩增(LAMP)诊断性传播感染:综述

IF 5.7 2区 生物学
Yasaman Ahmadi, Yejiong Yu, Zhanfeng Cui, Wei E. Huang, Monique I. Andersson
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引用次数: 0

摘要

性传播感染仍然是一个重大的公共卫生问题。鉴于许多性传播感染的无症状性质,诊断检测对于确定适当治疗、有效追踪和减少进一步传播的风险至关重要。核酸扩增试验(NAATs)是最敏感的,在资源充足的环境中使用最广泛。大多数可用的naat都是基于聚合酶链反应(PCR),这需要训练有素的人员和昂贵的设备,使得它在资源有限的环境中不切实际。环介导等温扩增(LAMP)已成为一种简单、快速、敏感和低成本的病原体检测替代方法,特别适合于即时检测(POCT)。在这篇综述中,我们评估了文献中报道的LAMP检测方法,用于检测与世界卫生组织(WHO)在2023年POCT中优先考虑的高发病率性传播感染相关的病原体。这些疾病包括淋病奈瑟菌、沙眼衣原体、阴道毛滴虫、苍白衣原体亚种以及其他常见的性传播感染,如单纯疱疹病毒、乙型肝炎病毒和人类免疫缺陷病毒(艾滋病毒)。对于每个LAMP检测,我们确定并总结了关键要素,如检测临床标本的类型和数量、选择的靶基因、检测系统、参考测试和临床结果。我们强调了这些检测的优点和局限性,并讨论了应解决的差距,以提高其对POCT的适用性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Loop-Mediated Isothermal Amplification (LAMP) for the Diagnosis of Sexually Transmitted Infections: A Review

Loop-Mediated Isothermal Amplification (LAMP) for the Diagnosis of Sexually Transmitted Infections: A Review

Sexually transmitted infections (STIs) remain a significant public health concern. Given the asymptomatic nature of many STIs, diagnostic testing is critical for determining the appropriate treatment, enabling effective tracing and reducing the risk of further transmission. Nucleic acid amplification tests (NAATs) are the most sensitive and the most widely used in well-resourced settings. The majority of available NAATs are based on polymerase chain reaction (PCR), which requires highly trained personnel and costly equipment, making it impractical for resource-limited settings. Loop-mediated isothermal amplification (LAMP) has emerged as a simple, rapid, sensitive and low-cost alternative for pathogen detection, particularly well-suited for point-of-care tests (POCT). In this review, we evaluate LAMP assays reported in the literature for the detection of pathogens linked to the high incidence STIs prioritised by the World Health Organization (WHO) for POCT in 2023. These include Neisseria gonorrhoeae, Chlamydia trachomatis, Trichomonas vaginalis, T. pallidum subspecies pallidum, as well as other common STIs such as herpes simplex virus, hepatitis B virus and human immunodeficiency virus (HIV). For each LAMP assay, we identified and summarised the key elements such as the type and number of tested clinical specimens, chosen target gene, detection system, reference test and clinical outcomes. We highlight the advantages and limitations of these assays and discuss the gaps that should be addressed to improve their applicability for POCT.

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来源期刊
Microbial Biotechnology
Microbial Biotechnology Immunology and Microbiology-Applied Microbiology and Biotechnology
CiteScore
11.20
自引率
3.50%
发文量
162
审稿时长
1 months
期刊介绍: Microbial Biotechnology publishes papers of original research reporting significant advances in any aspect of microbial applications, including, but not limited to biotechnologies related to: Green chemistry; Primary metabolites; Food, beverages and supplements; Secondary metabolites and natural products; Pharmaceuticals; Diagnostics; Agriculture; Bioenergy; Biomining, including oil recovery and processing; Bioremediation; Biopolymers, biomaterials; Bionanotechnology; Biosurfactants and bioemulsifiers; Compatible solutes and bioprotectants; Biosensors, monitoring systems, quantitative microbial risk assessment; Technology development; Protein engineering; Functional genomics; Metabolic engineering; Metabolic design; Systems analysis, modelling; Process engineering; Biologically-based analytical methods; Microbially-based strategies in public health; Microbially-based strategies to influence global processes
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