DLAT在高压电烧伤创伤后应激障碍大鼠海马组织铜突起和自噬中的作用

IF 3.2 3区医学 Q2 CRITICAL CARE MEDICINE

Burns Pub Date : 2025-04-22 DOI:10.1016/j.burns.2025.107519

Chenyang Ge, Xuegang Zhao, Jing Zhang, Peixuan Li, Jiawen Hao, Mengyuan Lu, Congying Li, Lili Ge, Lihong Tu, Qingfu Zhang

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引用次数: 0

摘要

高压电烧伤（HVEB）诱发创伤后应激障碍（PTSD）的机制尚不清楚。因此，我们旨在确定hveb诱导的创伤后应激障碍后海马组织的分子变化。方法采用GSE60303数据集，鉴定差异表达铜裂相关基因（DE-CRGs），并进行加权基因共表达网络分析。鉴定出核心基因和相关基因，并进行富集分析。建立HVEB创伤后应激障碍大鼠模型，进行行为学测试。对大鼠海马组织进行组织学检查，并检测相关蛋白和基因表达水平。结果在PTSD大鼠海马组织中鉴定出12个DE-CRGs，其中以DLAT为核心基因。dlt相关基因分析显示cAMP信号通路和自噬富集。行为测试证实，HVEB在大鼠中诱发了ptsd样行为。HVEB PTSD大鼠海马组织中DLAT表达降低，伴cuprotosis、cAMP通路、自噬相关基因表达改变。结论HVEB创伤后应激障碍大鼠海马组织中dlat减少。dlt的下调可能通过促进铜化、激活cAMP通路、增强自噬等途径促进HVEB大鼠ptsd样行为的发生。

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Role of DLAT in cuproptosis and autophagy in hippocampal tissue of PTSD rats with high-voltage electrical burns

Background

The mechanisms behind high-voltage electrical burn (HVEB)-induced post-traumatic stress disorder (PTSD) remain unclear. Accordingly, we aimed to identify the molecular changes in hippocampal tissue following HVEB-induced PTSD.

Methods

The GSE60303 dataset was used to identify differentially expressed cuproptosis-related genes (DE-CRGs) and to perform weighted gene co-expression network analysis. A core gene and associated genes were identified, followed by enrichment analysis. Additionally, a rat model of HVEB PTSD was established, and behavioral tests were conducted. Histological assessments and the evaluation of related protein and gene expression levels were performed on hippocampal tissue.

Results

Twelve DE-CRGs were identified in the hippocampal tissue of PTSD rats, with DLAT identified as the core gene. Analysis of DLAT-associated genes revealed enrichment in the cAMP signaling pathway and autophagy. Behavioral tests confirmed that HVEB induced PTSD-like behavior in rats. DLAT expression was decreased in the hippocampal tissue of HVEB PTSD rats, accompanied by changes in the expression of cuproptosis, cAMP pathway, and autophagy-related genes.

Conclusion

DLAT is reduced in the hippocampal tissue of HVEB PTSD rats. The downregulation of DLAT may contribute to the development of PTSD-like behaviors in HVEB rats by promoting cuproptosis, activating the cAMP pathway, and enhancing autophagy.

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来源期刊

Burns 医学-皮肤病学

CiteScore

4.50

自引率

18.50%

发文量

304

审稿时长

72 days

期刊介绍： Burns aims to foster the exchange of information among all engaged in preventing and treating the effects of burns. The journal focuses on clinical, scientific and social aspects of these injuries and covers the prevention of the injury, the epidemiology of such injuries and all aspects of treatment including development of new techniques and technologies and verification of existing ones. Regular features include clinical and scientific papers, state of the art reviews and descriptions of burn-care in practice. Topics covered by Burns include: the effects of smoke on man and animals, their tissues and cells; the responses to and treatment of patients and animals with chemical injuries to the skin; the biological and clinical effects of cold injuries; surgical techniques which are, or may be relevant to the treatment of burned patients during the acute or reconstructive phase following injury; well controlled laboratory studies of the effectiveness of anti-microbial agents on infection and new materials on scarring and healing; inflammatory responses to injury, effectiveness of related agents and other compounds used to modify the physiological and cellular responses to the injury; experimental studies of burns and the outcome of burn wound healing; regenerative medicine concerning the skin.