壳聚糖和SS31肽双稳定硒纳米粒子:解决不稳定性,增强ROS消除,抑制炎症,对抗细菌感染

IF 5.6 2区 医学 Q1 BIOPHYSICS
Panpan Ming , Yuwen Wei , Yawen Zhu , Kang Li , Wenqing Zhu , Jing Qiu
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引用次数: 0

摘要

硒纳米颗粒(SeNPs)由于其抗炎、抗氧化和组织再生的特性,在控制炎症微环境方面具有重要的前景。然而,它们的稳定性差限制了实际应用。为了解决这个问题,我们开发了一种新的纳米复合材料,将SeNPs与壳聚糖和线粒体靶向肽SS31 (CS/SS31-SeNPs)通过氧化还原合成方法共同稳定。经动态光散射、TEM、EDX、XPS和TGA分析证实,优化后的CS/SS31-SeNPs具有均匀的球形结构(直径82 nm, zeta电位+48 mV)和优异的稳定性(超过90天没有聚集)。纳米复合材料在体外和体内均表现出增强的活性氧(ROS)清除效率。在硫酸铜诱导的斑马鱼炎症模型中,CS/SS31-SeNPs预处理分别减少了38.07 %和43.56 %的中性粒细胞和巨噬细胞募集,优于裸SeNPs。此外,CS/SS31-SeNPs对金黄色葡萄球菌表现出优异的抗菌活性,在64 μM下实现了近乎完全的生长抑制。机制研究表明,抗菌作用源于靶向肽聚糖合成中保守的MraY酶。分子对接表明CS/SS31-SeNPs与MraY的尿嘧啶口袋和邻近位点稳定结合(-15.6 kcal/mol),这一机制与传统抗生素不同,表明具有广谱潜力。通过将壳聚糖的抗菌特性与SS31的线粒体靶向性协同结合,CS/SS31-SeNPs克服了SeNPs的不稳定性,同时提高了其治疗效果。这种多功能平台为治疗口腔-颅面炎症和感染性疾病提供了一种有希望的策略,对减轻抗生素耐药性具有重要意义。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Dual-stabilized selenium nanoparticles with chitosan and SS31 peptide: Resolving instability for enhancing ROS elimination, suppressing inflammation, and combating bacterial infections
Selenium nanoparticles (SeNPs) hold significant promise for managing inflammatory microenvironments due to their anti-inflammatory, antioxidant, and tissue-regenerative properties. However, their poor stability limits practical applications. To address this, we developed a novel nanocomposite by co-stabilizing SeNPs with chitosan and the mitochondria-targeting peptide SS31 (CS/SS31-SeNPs) via a redox synthesis method. The optimized CS/SS31-SeNPs exhibited a uniform spherical structure (82 nm diameter, +48 mV zeta potential) and exceptional stability (no aggregation over 90 days), as confirmed by dynamic light scattering, TEM, EDX, XPS and TGA analyses. The nanocomposites demonstrated enhanced reactive oxygen species (ROS) scavenging efficiency in vitro and in vivo. In a copper sulfate-induced zebrafish inflammation model, CS/SS31-SeNPs pretreatment reduced neutrophil and macrophage recruitment by 38.07 % and 43.56 %, respectively, outperforming bare SeNPs. Furthermore, CS/SS31-SeNPs exhibited superior antibacterial activity against Staphylococcus aureus, achieving near-complete growth inhibition at 64 μM. Mechanistic studies revealed that the antibacterial action stems from targeting the conserved MraY enzyme in peptidoglycan synthesis. Molecular docking indicated stable binding (-15.6 kcal/mol) of CS/SS31-SeNPs to MraY's uracil pocket and adjacent sites-a mechanism distinct from conventional antibiotics, suggesting broad-spectrum potential. By synergistically integrating chitosan's antibacterial properties with SS31's mitochondrial targeting, CS/SS31-SeNPs overcome SeNPs instability while amplifying their therapeutic efficacy. This multifunctional platform offers a promising strategy for treating oral-craniofacial inflammatory and infectious diseases, with implications for antibiotic resistance mitigation.
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来源期刊
Colloids and Surfaces B: Biointerfaces
Colloids and Surfaces B: Biointerfaces 生物-材料科学:生物材料
CiteScore
11.10
自引率
3.40%
发文量
730
审稿时长
42 days
期刊介绍: Colloids and Surfaces B: Biointerfaces is an international journal devoted to fundamental and applied research on colloid and interfacial phenomena in relation to systems of biological origin, having particular relevance to the medical, pharmaceutical, biotechnological, food and cosmetic fields. Submissions that: (1) deal solely with biological phenomena and do not describe the physico-chemical or colloid-chemical background and/or mechanism of the phenomena, and (2) deal solely with colloid/interfacial phenomena and do not have appropriate biological content or relevance, are outside the scope of the journal and will not be considered for publication. The journal publishes regular research papers, reviews, short communications and invited perspective articles, called BioInterface Perspectives. The BioInterface Perspective provide researchers the opportunity to review their own work, as well as provide insight into the work of others that inspired and influenced the author. Regular articles should have a maximum total length of 6,000 words. In addition, a (combined) maximum of 8 normal-sized figures and/or tables is allowed (so for instance 3 tables and 5 figures). For multiple-panel figures each set of two panels equates to one figure. Short communications should not exceed half of the above. It is required to give on the article cover page a short statistical summary of the article listing the total number of words and tables/figures.
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