Panpan Ming , Yuwen Wei , Yawen Zhu , Kang Li , Wenqing Zhu , Jing Qiu
{"title":"壳聚糖和SS31肽双稳定硒纳米粒子:解决不稳定性,增强ROS消除,抑制炎症,对抗细菌感染","authors":"Panpan Ming , Yuwen Wei , Yawen Zhu , Kang Li , Wenqing Zhu , Jing Qiu","doi":"10.1016/j.colsurfb.2025.114749","DOIUrl":null,"url":null,"abstract":"<div><div>Selenium nanoparticles (SeNPs) hold significant promise for managing inflammatory microenvironments due to their anti-inflammatory, antioxidant, and tissue-regenerative properties. However, their poor stability limits practical applications. To address this, we developed a novel nanocomposite by co-stabilizing SeNPs with chitosan and the mitochondria-targeting peptide SS31 (CS/SS31-SeNPs) via a redox synthesis method. The optimized CS/SS31-SeNPs exhibited a uniform spherical structure (82 nm diameter, +48 mV zeta potential) and exceptional stability (no aggregation over 90 days), as confirmed by dynamic light scattering, TEM, EDX, XPS and TGA analyses. The nanocomposites demonstrated enhanced reactive oxygen species (ROS) scavenging efficiency in vitro and in vivo. In a copper sulfate-induced zebrafish inflammation model, CS/SS31-SeNPs pretreatment reduced neutrophil and macrophage recruitment by 38.07 % and 43.56 %, respectively, outperforming bare SeNPs. Furthermore, CS/SS31-SeNPs exhibited superior antibacterial activity against <em>Staphylococcus aureus</em>, achieving near-complete growth inhibition at 64 μM. Mechanistic studies revealed that the antibacterial action stems from targeting the conserved MraY enzyme in peptidoglycan synthesis. Molecular docking indicated stable binding (-15.6 kcal/mol) of CS/SS31-SeNPs to MraY's uracil pocket and adjacent sites-a mechanism distinct from conventional antibiotics, suggesting broad-spectrum potential. By synergistically integrating chitosan's antibacterial properties with SS31's mitochondrial targeting, CS/SS31-SeNPs overcome SeNPs instability while amplifying their therapeutic efficacy. This multifunctional platform offers a promising strategy for treating oral-craniofacial inflammatory and infectious diseases, with implications for antibiotic resistance mitigation.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"253 ","pages":"Article 114749"},"PeriodicalIF":5.6000,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Dual-stabilized selenium nanoparticles with chitosan and SS31 peptide: Resolving instability for enhancing ROS elimination, suppressing inflammation, and combating bacterial infections\",\"authors\":\"Panpan Ming , Yuwen Wei , Yawen Zhu , Kang Li , Wenqing Zhu , Jing Qiu\",\"doi\":\"10.1016/j.colsurfb.2025.114749\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Selenium nanoparticles (SeNPs) hold significant promise for managing inflammatory microenvironments due to their anti-inflammatory, antioxidant, and tissue-regenerative properties. However, their poor stability limits practical applications. To address this, we developed a novel nanocomposite by co-stabilizing SeNPs with chitosan and the mitochondria-targeting peptide SS31 (CS/SS31-SeNPs) via a redox synthesis method. The optimized CS/SS31-SeNPs exhibited a uniform spherical structure (82 nm diameter, +48 mV zeta potential) and exceptional stability (no aggregation over 90 days), as confirmed by dynamic light scattering, TEM, EDX, XPS and TGA analyses. The nanocomposites demonstrated enhanced reactive oxygen species (ROS) scavenging efficiency in vitro and in vivo. In a copper sulfate-induced zebrafish inflammation model, CS/SS31-SeNPs pretreatment reduced neutrophil and macrophage recruitment by 38.07 % and 43.56 %, respectively, outperforming bare SeNPs. Furthermore, CS/SS31-SeNPs exhibited superior antibacterial activity against <em>Staphylococcus aureus</em>, achieving near-complete growth inhibition at 64 μM. Mechanistic studies revealed that the antibacterial action stems from targeting the conserved MraY enzyme in peptidoglycan synthesis. Molecular docking indicated stable binding (-15.6 kcal/mol) of CS/SS31-SeNPs to MraY's uracil pocket and adjacent sites-a mechanism distinct from conventional antibiotics, suggesting broad-spectrum potential. By synergistically integrating chitosan's antibacterial properties with SS31's mitochondrial targeting, CS/SS31-SeNPs overcome SeNPs instability while amplifying their therapeutic efficacy. 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Dual-stabilized selenium nanoparticles with chitosan and SS31 peptide: Resolving instability for enhancing ROS elimination, suppressing inflammation, and combating bacterial infections
Selenium nanoparticles (SeNPs) hold significant promise for managing inflammatory microenvironments due to their anti-inflammatory, antioxidant, and tissue-regenerative properties. However, their poor stability limits practical applications. To address this, we developed a novel nanocomposite by co-stabilizing SeNPs with chitosan and the mitochondria-targeting peptide SS31 (CS/SS31-SeNPs) via a redox synthesis method. The optimized CS/SS31-SeNPs exhibited a uniform spherical structure (82 nm diameter, +48 mV zeta potential) and exceptional stability (no aggregation over 90 days), as confirmed by dynamic light scattering, TEM, EDX, XPS and TGA analyses. The nanocomposites demonstrated enhanced reactive oxygen species (ROS) scavenging efficiency in vitro and in vivo. In a copper sulfate-induced zebrafish inflammation model, CS/SS31-SeNPs pretreatment reduced neutrophil and macrophage recruitment by 38.07 % and 43.56 %, respectively, outperforming bare SeNPs. Furthermore, CS/SS31-SeNPs exhibited superior antibacterial activity against Staphylococcus aureus, achieving near-complete growth inhibition at 64 μM. Mechanistic studies revealed that the antibacterial action stems from targeting the conserved MraY enzyme in peptidoglycan synthesis. Molecular docking indicated stable binding (-15.6 kcal/mol) of CS/SS31-SeNPs to MraY's uracil pocket and adjacent sites-a mechanism distinct from conventional antibiotics, suggesting broad-spectrum potential. By synergistically integrating chitosan's antibacterial properties with SS31's mitochondrial targeting, CS/SS31-SeNPs overcome SeNPs instability while amplifying their therapeutic efficacy. This multifunctional platform offers a promising strategy for treating oral-craniofacial inflammatory and infectious diseases, with implications for antibiotic resistance mitigation.
期刊介绍:
Colloids and Surfaces B: Biointerfaces is an international journal devoted to fundamental and applied research on colloid and interfacial phenomena in relation to systems of biological origin, having particular relevance to the medical, pharmaceutical, biotechnological, food and cosmetic fields.
Submissions that: (1) deal solely with biological phenomena and do not describe the physico-chemical or colloid-chemical background and/or mechanism of the phenomena, and (2) deal solely with colloid/interfacial phenomena and do not have appropriate biological content or relevance, are outside the scope of the journal and will not be considered for publication.
The journal publishes regular research papers, reviews, short communications and invited perspective articles, called BioInterface Perspectives. The BioInterface Perspective provide researchers the opportunity to review their own work, as well as provide insight into the work of others that inspired and influenced the author. Regular articles should have a maximum total length of 6,000 words. In addition, a (combined) maximum of 8 normal-sized figures and/or tables is allowed (so for instance 3 tables and 5 figures). For multiple-panel figures each set of two panels equates to one figure. Short communications should not exceed half of the above. It is required to give on the article cover page a short statistical summary of the article listing the total number of words and tables/figures.