测定尿液、血清和唾液样本中尼古丁和尼古丁代谢物水平的方法验证

IF 1.8 4区 医学 Q4 BIOCHEMICAL RESEARCH METHODS
Gülsüm Abuşoğlu, Duygu Eryavuz Onmaz, Ali Ünlü, Fatma Hümeyra Yerlikaya Aydemir, Sedat Abuşoğlu
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引用次数: 0

摘要

在临床实践中,监测体液中的尼古丁及其代谢物对于评估烟草接触和相关毒性至关重要。由于液相色谱-串联质谱(LC-MS /MS)在检测低浓度分析物方面具有很高的灵敏度,因此本文采用液相色谱-串联质谱(LC-MS /MS)技术对血清、尿液和唾液中的尼古丁及其衍生物进行了定量分析。对有吸烟习惯和没有吸烟习惯的人的样本进行了比较分析。LC-MS /MS方法采用API 3200三重四极杆仪器,配以Phenomenex Luna C18色谱柱。通过评估灵敏度参数,包括所有矩阵中每种分析物的LOD和LLOQ,证明了方法的灵敏度。定量限水平上的测定间变异性和偏倚值保持在20%以下。血清中尼古丁的lod - loq范围为0.02 ~ 0.05 μg/L,可替宁为0.32 ~ 0.95 μg/L, 3-OH可替宁为0.07 ~ 0.25 μg/L,诺可替宁为0.22 ~ 0.69 μg/L。尿液中分别为0.03 ~ 0.10、0.23 ~ 0.82、0.06 ~ 0.14、0.20 ~ 0.55 μg/L,唾液中分别为0.12 ~ 1.59、0.22 ~ 2.34、0.10 ~ 0.17、0.33 ~ 3.01 μg/L。所提出的方法是有效的,特异性的,并且适用于常规临床工作流程,为监测烟草相关暴露提供了一个有价值的工具。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Method Validation for the Determination of Nicotine and Nicotine Metabolite Levels in Urine, Serum, and Saliva Samples

Monitoring nicotine and its metabolites in bodily fluids is crucial for assessing tobacco exposure and related toxicity in clinical practice. Due to its high sensitivity for detecting low-level analytes, liquid chromatography–tandem mass spectrometry (LC–MS/MS) was employed to enhance a robust and accurate analytical procedure for quantifying nicotine and its derivatives in serum, urine, and saliva. Comparative analysis was conducted on samples collected from individuals with and without smoking habits. The LC–MS/MS method utilized an API 3200 triple quadrupole instrument paired with a Phenomenex Luna C18 column. Method sensitivity was demonstrated through evaluation of sensitivity parameters, including LOD and LLOQ for each analyte in all matrices. Inter-assay variability and bias values at the LLOQ level were kept below 20%. In serum, the LOD–LLOQ ranges were 0.02–0.05 μg/L for nicotine, 0.32–0.95 μg/L for cotinine, 0.07–0.25 μg/L for 3-OH cotinine, and 0.22–0.69 μg/L for norcotinine. In urine, the respective values were 0.03–0.10, 0.23–0.82, 0.06–0.14, and 0.20–0.55 μg/L, and in saliva, the values were 0.12–1.59, 0.22–2.34, 0.10–0.17, and 0.33–3.01 μg/L. The proposed method is efficient, specific, and adaptable to routine clinical workflows, providing a valuable tool for monitoring tobacco-related exposure.

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来源期刊
Biomedical Chromatography
Biomedical Chromatography 生物-分析化学
CiteScore
3.60
自引率
5.60%
发文量
268
审稿时长
2.3 months
期刊介绍: Biomedical Chromatography is devoted to the publication of original papers on the applications of chromatography and allied techniques in the biological and medical sciences. Research papers and review articles cover the methods and techniques relevant to the separation, identification and determination of substances in biochemistry, biotechnology, molecular biology, cell biology, clinical chemistry, pharmacology and related disciplines. These include the analysis of body fluids, cells and tissues, purification of biologically important compounds, pharmaco-kinetics and sequencing methods using HPLC, GC, HPLC-MS, TLC, paper chromatography, affinity chromatography, gel filtration, electrophoresis and related techniques.
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