枸杞多糖的结构表征西南。及其对草酸钙肾结石的抑制作用

IF 6.7 1区医学 Q1 CHEMISTRY, MEDICINAL

Phytomedicine Pub Date : 2025-04-10 DOI:10.1016/j.phymed.2025.156734

Chun-Yao Li , Quan Zhang , Xin-Yu Shi , Jun Long , Bang-Xian Yu , Xue-Wu Chen , Ling-Hong Huang , Xin-Yuan Sun

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引用次数: 0

摘要

肾结石是一种常见的异常矿化疾病，具有高发病率和复发率的特点。目前肾结石的药物干预主要依赖于柠檬酸钾（PC），但其临床疗效仍然有限。日本枸杞（属）西南。具有食用和药用价值，是中医治疗尿路结石最常用的草药之一；然而，其物质基础和潜在机制尚不清楚。方法采用热水提取纯化方法，得到分子量为12.9 kDa的枇杷多糖（LJP）。对LJP的结构进行了表征，并对其在抑制肾结石形成中的作用进行了研究。结果sljp主要由Glc、Gal- ua、Glc- ua、Gal、Rha和Ara单糖组成，主链主要由→4)-α-d- glcp -(1→键组成，还有少量的→2)-α-d- glcp -(1→，→2,6)-α-d- glcp -(1→，→3,6)-β-d- glcp -(1→，→4,6)-β-d- glcp -(1→，→3)-α-d- glcp -(1→，→3)-α-d- glcp -(1→，→3)-α-d- glcp -（1→）键组成。LJP能够特异性吸附在高能量（1¯01）的晶体表面，抑制一水草酸钙（COM）的生长，显著减小晶体尺寸，促进从COM到二水草酸钙（COD）的相变。此外，它有效地抑制晶体粘附和内吞作用。LJP还表现出优异的抗氧化性能，减轻纳米com晶体引起的细胞氧化应激，减少线粒体、溶酶体和DNA损伤，抑制细胞凋亡。此外，LJP可以在大鼠肾脏中有效富集，显著抑制体内草酸钙（CaOx）晶体的形成，减轻肾脏损伤。代谢组学分析显示LJP主要影响柠檬酸循环和嘌呤代谢途径。与传统的结石治疗药物PC相比，LJP在调节CaOx晶体形态和细胞保护方面表现出优越的性能。结论ljp是治疗肾结石的一种有前景的治疗方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Structural characterization of polysaccharides from Lygodium japonicum (Thunb.) Sw. and its inhibition ability in calcium oxalate renal stone

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Structural characterization of polysaccharides from Lygodium japonicum (Thunb.) Sw. and its inhibition ability in calcium oxalate renal stone

Background

Kidney stone is a prevalent abnormal mineralization disease characterized by high incidence and recurrence rates. Current pharmacological interventions for kidney stone predominantly rely on potassium citrate (PC), yet its clinical efficacy remains limited. Lygodium japonicum (Thunb.) Sw., which possesses both edible and medicinal values, is one of the most commonly used herbs in traditional Chinese medicine for treating urinary tract stones; however, its material basis and underlying mechanisms remain unclear.

Methods

a l. japonicum polysaccharide (LJP) with a molecular weight of 12.9 kDa was obtained through hot-water extraction and purification. The structure of LJP was characterized, and its role in inhibiting kidney stone formation was investigated.

Results

LJP primarily consists of Glc, Gal-UA, Glc-UA, Gal, Rha, and Ara monosaccharides, with the main chain mainly composed of →4)-α-d-Glcp-(1→ linkages, along with minor amounts of →2)-α-d-Glcp-(1→, →2,6)-α-d-Glcp-(1→, →3,6)-β-d-Glcp-(1→, →4,6)-β-d-Glcp-(1→, →3)-α-d-Glcp-(1→. LJP is able to specifically adsorb onto high-energy (

\bar{1} 01

) crystal surfaces to inhibit calcium oxalate monohydrate (COM) growth, significantly reducing crystal size and promoting phase conversion from COM to calcium oxalate dihydrate (COD). Additionally, it effectively inhibits crystal adhesion and endocytosis. LJP also exhibits excellent antioxidant properties, mitigating cellular oxidative stress induced by nano-COM crystals, reducing mitochondrial, lysosomal, and DNA damage, and inhibiting cell apoptosis. In addition, LJP can be effectively enriched in rat kidneys, significantly inhibiting calcium oxalate (CaOx) crystal formation in vivo and reducing renal injury. Metabolomic profiling revealed that LJP mainly affects the citric acid cycle and purine metabolic pathways. Compared to PC, a conventional stone treatment drug, LJP demonstrates superior performance in modulating CaOx crystalline form and cytoprotection.

Conclusion

LJP may serve as a promising therapeutic option for the treatment of renal stones.

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来源期刊

Phytomedicine 医学-药学

CiteScore

10.30

自引率

5.10%

发文量

670

审稿时长

91 days

期刊介绍： Phytomedicine is a therapy-oriented journal that publishes innovative studies on the efficacy, safety, quality, and mechanisms of action of specified plant extracts, phytopharmaceuticals, and their isolated constituents. This includes clinical, pharmacological, pharmacokinetic, and toxicological studies of herbal medicinal products, preparations, and purified compounds with defined and consistent quality, ensuring reproducible pharmacological activity. Founded in 1994, Phytomedicine aims to focus and stimulate research in this field and establish internationally accepted scientific standards for pharmacological studies, proof of clinical efficacy, and safety of phytomedicines.