绵羊精液PRDX6水平与冷冻性的相关性研究

IF 2.4 2区农林科学 Q3 REPRODUCTIVE BIOLOGY

Theriogenology Pub Date : 2025-04-27 DOI:10.1016/j.theriogenology.2025.117461

Erhan Hai , Xue Bai , Yukun Song , Boyuan Li , Jian Zhang , Jiaxin Zhang

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引用次数: 0

摘要

绵羊精液冷冻性能的个体差异可能与精子的抗氧化水平有关。PRDX6是精子中重要的抗氧化蛋白，但其与绵羊精液冷冻性的关系尚不清楚。本研究以湖羊为研究对象，研究了不同冷冻性精子中PRDX6的含量。PRDX6水平与精子冷冻性呈正相关。此外，在高抗冻性（HF）和低抗冻性（LF）精子中添加不同浓度的PRDX6重组蛋白，结果显示，20 μg/ml PRDX6重组蛋白显著改善了高抗冻性（HF）和低抗冻性（LF）精子的各项质量参数，并缩小了精子冷冻性能的差异。最后，在稀释液中加入PRDX6过氧化物酶活性抑制剂（乙酸）或钙非依赖性磷脂酶A2 （iPLA2）活性抑制剂（MJ33）可显著降低精子的冷冻性参数。这表明PRDX6通过两种途径保护精子免受冷冻损伤。综上所述，本研究揭示了PRDX6在绵羊精液冷冻性能中的重要作用。提示提高精子中PRDX6的水平可能是提高冷冻精液质量的有效策略。未来的研究可以进一步探索PRDX6水平调控的分子机制，为优化绵羊精液冷冻保存技术提供科学依据。

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Correlation between PRDX6 levels and freezability properties of sheep semen

Individual variations in the freezability properties of sheep semen may be related to the antioxidant levels of sperm. PRDX6 is a key antioxidant protein in sperm, but its relationship with the freezability properties of sheep semen remains unclear.

This study, using Hu sheep as the research subject, investigated the levels of PRDX6 in sperm with different freezability properties. A positive correlation was found between the levels of PRDX6 and sperm freezability. Furthermore, when different concentrations of PRDX6 recombinant protein were added to high-freezing-resistant (HF) and low-freezing-resistant (LF) sperm, it was revealed that 20 μg/ml of PRDX6 recombinant protein significantly improved various sperm quality parameters in both HF and LF sperm and reduced the differences in sperm freezability properties. Finally, adding a PRDX6 peroxidase activity inhibitor (Ethacrynic acid) or a calcium - independent phospholipase A2 (iPLA2) activity inhibitor (MJ33) to the diluent significantly reduced the freezability parameters of sperm. This indicates that PRDX6 protects sperm from freezing damage through two pathways. In summary, this study reveals the crucial role of PRDX6 in the freezability properties of sheep semen. It suggests that increasing the levels of PRDX6 in sperm may be an effective strategy to improve the quality of cryopreserved semen. Future research can further explore the molecular mechanisms regulating the levels of PRDX6, providing a scientific basis for the optimization of sheep semen cryopreservation techniques.

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来源期刊

Theriogenology 农林科学-生殖生物学

CiteScore

5.50

自引率

14.30%

发文量

387

审稿时长

72 days

期刊介绍： Theriogenology provides an international forum for researchers, clinicians, and industry professionals in animal reproductive biology. This acclaimed journal publishes articles on a wide range of topics in reproductive and developmental biology, of domestic mammal, avian, and aquatic species as well as wild species which are the object of veterinary care in research or conservation programs.