Influence of contamination of saliva, blood, and their mixture on shear bond strength between tricalcium silicate-based bioceramic (Biodentine®) and resin-based dental composite

The present study investigated the effect of different contaminations of saliva, blood, and a mixture of saliva and blood on the invitro shear bond strength between tricalcium silicate-based bioceramic and a resin-based dental composite material. Forty cylindrical acrylic molds with the diameter of 30 mm and height of 13 mm were prepared. Then, a hole with an inner diameter of 5 mm and depth of 3 mm was formed by machining the center of each acrylic mold and filled with Biodentine. Forty specimens were randomized and allocated into four groups (n = 10) as control, saliva, blood, and saliva + blood. A resin-based dental composite material was bonded to contamination-free (control group) and contaminated surfaces of Biodentine using a universal adhesive. Shear bond strength tests were conducted using a universal testing machine (Instron 3382, Canton, MA, USA). The failure modes of debonded sample surfaces were identified using stereo-microscope. Scanning electron microscope images of the selected samples were also analyzed to identify the failure modes and fracture mechanisms in detail. Data were analyzed using IBM SPSS v23. A statistically significant difference was observed among the median values of the shear bond strength of the groups (p < 0.006). The control group had the highest mean shear bond strength value. Although the control group was statistically similar to the saliva group, it differed from the blood and saliva + blood groups. All groups, except the control, showed statistically similar shear bond strength values. While the control group without any contaminant exhibited a mostly cohesive failure mode (60%), the groups contaminated with saliva, blood, and saliva + blood generally exhibited an adhesive failure mode (%50). The control group showed the highest median shear bond strength value among the groups, which means that any contamination of Biodentine with saliva, blood, or the mixture of saliva and blood decreased statistically and considerably shear bond strength between tricalcium silicate-based bioceramic and resin-based dental composite.

Ethical Registration Approval Number: Number 64529847/30 and protocol code 2024/27