Yun Yang , Jinxin Wang , Lulu Li , Haofei Yang , Yu Quan , Jason Jia Shun Liao , Wei Ouyang , Gang Yu , Li Ling
{"title":"释放基于受体的检测在高通量检测中的力量:评估酶联免疫吸附法在大规模抗生素监测中的可行性","authors":"Yun Yang , Jinxin Wang , Lulu Li , Haofei Yang , Yu Quan , Jason Jia Shun Liao , Wei Ouyang , Gang Yu , Li Ling","doi":"10.1016/j.emcon.2025.100511","DOIUrl":null,"url":null,"abstract":"<div><div>Antibiotics are detected in aquatic environments with heterogeneity in their occurrence and associated risk levels. Thus, high spatiotemporal resolution monitoring in a large scale is essential to better cope with their risks. Mass spectrometry (MS) techniques are sensitive and precise, yet complex, expensive, and time-consuming for antibiotic detection. The enzyme-linked immunosorbent assay (ELISA) is among a mature receptor-based assays that offer a cost-effective alternative and are particularly notable for their high throughput analytical capabilities. However, its high throughput power on environmental monitoring is underutilized. ELISA is remarkably rapid (3800–37,000 tests per day), inexpensive ($1.8 per test, capital costs ranging from $35,000 to $270,000), and ready-to-use (97 commercial kits available) for detecting frequently reported antibiotics. Adopting solid phase extraction decreases their limits of detection to as low as 0.125 ng/L. Their quantification results are robust as they also generally agreed well with those of MS methods. A conservative way at present is to use ELISA for initial screening of large numbers of samples, with subsequent quantification of a small proportion of “positive” samples through MS methods. Yet, the applicability of ELISA can be further improved, such as developing a standardized quantification procedure for ELISA and microfluid chip-based ELISA kits and instruments.</div></div>","PeriodicalId":11539,"journal":{"name":"Emerging Contaminants","volume":"11 3","pages":"Article 100511"},"PeriodicalIF":5.3000,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Unleashing the power of receptor-based assay on high-throughput detection: Assessing the feasibility of enzyme-linked immunosorbent assay on large-scale antibiotic monitoring\",\"authors\":\"Yun Yang , Jinxin Wang , Lulu Li , Haofei Yang , Yu Quan , Jason Jia Shun Liao , Wei Ouyang , Gang Yu , Li Ling\",\"doi\":\"10.1016/j.emcon.2025.100511\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Antibiotics are detected in aquatic environments with heterogeneity in their occurrence and associated risk levels. Thus, high spatiotemporal resolution monitoring in a large scale is essential to better cope with their risks. Mass spectrometry (MS) techniques are sensitive and precise, yet complex, expensive, and time-consuming for antibiotic detection. The enzyme-linked immunosorbent assay (ELISA) is among a mature receptor-based assays that offer a cost-effective alternative and are particularly notable for their high throughput analytical capabilities. However, its high throughput power on environmental monitoring is underutilized. ELISA is remarkably rapid (3800–37,000 tests per day), inexpensive ($1.8 per test, capital costs ranging from $35,000 to $270,000), and ready-to-use (97 commercial kits available) for detecting frequently reported antibiotics. Adopting solid phase extraction decreases their limits of detection to as low as 0.125 ng/L. Their quantification results are robust as they also generally agreed well with those of MS methods. A conservative way at present is to use ELISA for initial screening of large numbers of samples, with subsequent quantification of a small proportion of “positive” samples through MS methods. Yet, the applicability of ELISA can be further improved, such as developing a standardized quantification procedure for ELISA and microfluid chip-based ELISA kits and instruments.</div></div>\",\"PeriodicalId\":11539,\"journal\":{\"name\":\"Emerging Contaminants\",\"volume\":\"11 3\",\"pages\":\"Article 100511\"},\"PeriodicalIF\":5.3000,\"publicationDate\":\"2025-04-25\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Emerging Contaminants\",\"FirstCategoryId\":\"1087\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2405665025000459\",\"RegionNum\":2,\"RegionCategory\":\"环境科学与生态学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"ENVIRONMENTAL SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Emerging Contaminants","FirstCategoryId":"1087","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2405665025000459","RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENVIRONMENTAL SCIENCES","Score":null,"Total":0}
Unleashing the power of receptor-based assay on high-throughput detection: Assessing the feasibility of enzyme-linked immunosorbent assay on large-scale antibiotic monitoring
Antibiotics are detected in aquatic environments with heterogeneity in their occurrence and associated risk levels. Thus, high spatiotemporal resolution monitoring in a large scale is essential to better cope with their risks. Mass spectrometry (MS) techniques are sensitive and precise, yet complex, expensive, and time-consuming for antibiotic detection. The enzyme-linked immunosorbent assay (ELISA) is among a mature receptor-based assays that offer a cost-effective alternative and are particularly notable for their high throughput analytical capabilities. However, its high throughput power on environmental monitoring is underutilized. ELISA is remarkably rapid (3800–37,000 tests per day), inexpensive ($1.8 per test, capital costs ranging from $35,000 to $270,000), and ready-to-use (97 commercial kits available) for detecting frequently reported antibiotics. Adopting solid phase extraction decreases their limits of detection to as low as 0.125 ng/L. Their quantification results are robust as they also generally agreed well with those of MS methods. A conservative way at present is to use ELISA for initial screening of large numbers of samples, with subsequent quantification of a small proportion of “positive” samples through MS methods. Yet, the applicability of ELISA can be further improved, such as developing a standardized quantification procedure for ELISA and microfluid chip-based ELISA kits and instruments.
期刊介绍:
Emerging Contaminants is an outlet for world-leading research addressing problems associated with environmental contamination caused by emerging contaminants and their solutions. Emerging contaminants are defined as chemicals that are not currently (or have been only recently) regulated and about which there exist concerns regarding their impact on human or ecological health. Examples of emerging contaminants include disinfection by-products, pharmaceutical and personal care products, persistent organic chemicals, and mercury etc. as well as their degradation products. We encourage papers addressing science that facilitates greater understanding of the nature, extent, and impacts of the presence of emerging contaminants in the environment; technology that exploits original principles to reduce and control their environmental presence; as well as the development, implementation and efficacy of national and international policies to protect human health and the environment from emerging contaminants.