鼠李糖脂无细胞肉汤在土壤中潜在有毒金属污染修复中的应用——金属污染物吸附研究

IF 3.8 4区环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES

Water, Air, & Soil Pollution Pub Date : 2025-04-30 DOI:10.1007/s11270-025-08061-4

Gislene S. Souza, Joane A. Alves, Emanuele P. Lima, Lucas M. S. Pimentel, Giulian C. S. Sá, Charlie G. Gomes, Emilly C. Silva, Sidnei C. Santos

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引用次数: 0

摘要

工业增长和人为活动导致有毒物质的不当释放，例如潜在危险的金属物种进入土壤并破坏营养链。为了解决这一紧迫的问题，本研究的重点是开发环境友好的土壤修复方法。研究了铜绿假单胞菌BM02生产的鼠李糖脂无细胞肉汤（RCFB）去除污染土壤中Cd2+、Ni2+和Cr2+的效果。此外，使用未经纯化的生物表面活性剂进行土壤洗涤是一种新颖的方法，使该过程更加经济和可持续。采用24 × 2因子设计的中心复合设计（CCD）对RCFB进行了优化，评估了硫酸镁、硫酸铵和银足螺（Carapa guianensis Aublet）生物质废弃物的浓度以及孵育时间等自变量。对RCFB的官能团和表面活性剂性能进行了表征，RCFB对Cd2+、Ni2+和Cr2+的理论最大吸附量分别为149.2±0.10 mg/Kg、120.1±0.14 mg/Kg和118.13±0.12 mg/Kg。吸附过程按照拟一阶模型进行，根据观测数据，对所有金属离子的吸附结果都符合Langmuir模型。这些研究结果表明，RCFB可作为一种有前景的螯合洗脱剂用于重金属污染土壤的生物修复。生物表面活性剂具有可持续、经济和快速的制备过程，是一种替代化学表面活性剂的生态技术，具有创新意义。此外，游离肉汤的应用避免了生物表面活性剂提取和纯化过程中有毒溶剂的使用，使其成为更可持续和经济的选择。图形抽象

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Application of Rhamnolipid-Cell Free Broth in Remediation of Soil Contaminated with Potentially Toxic Metals: A Study of Metal Contaminant Adsorption

Industrial growth and anthropogenic activities have resulted in the improper release of toxic substances, such as potentially hazardous metallic species into the soil and disrupting the trophic chain. To address this pressing issue, this research has focused on developing environmentally friendly methodologies for soil remediation. This study investigated the efficacy of rhamnolipid cell-free broth (RCFB), derived from the production by the bacterium Pseudomonas aeruginosa BM02, in removing Cd²⁺, Ni²⁺, and Cr²⁺ species from contaminated soil. Furthermore, there is the novelty of applying soil washing with unpurified biosurfactant, making the process much more economical and sustainable. The RCFB was optimized using a central composite design (CCD) with a 2⁴ × 2 factorial arrangement, evaluating independent variables such as concentration of magnesium sulfate, ammonium sulfate, and andiroba (Carapa guianensis Aublet) biomass waste, along with incubation time. Characterization of the functional groups and surfactant properties of RCFB was conducted, and the theoretical maximum adsorption capacities of RCFB for Cd²⁺, Ni²⁺, and Cr²⁺ were 149.2 ± 0.10 mg/Kg, 120.1 ± 0.14 mg/Kg and 118.13 ± 0.12 mg/Kg, respectively. The removal process followed in accordance with the pseudo first order model and, according to the observed data, a satisfactory fit of the adsorption results to the Langmuir model was obtained for all metal ions. These findings suggest that RCFB can be used as a promising chelating eluent for bioremediation of contaminated soils by metallic species. This research is innovative due to the sustainable, economical and rapid process of obtaining the biosurfactant, emerging as an ecological alternative to chemical surfactants, which can be toxic and persistent in the environment. In addition, the application of free broth avoids the use of toxic solvents associated with the extraction and purification process of the biosurfactant, making it a more sustainable and economical choice.

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来源期刊

Water, Air, & Soil Pollution 环境科学-环境科学

CiteScore

4.50

自引率

6.90%

发文量

448

审稿时长

2.6 months

期刊介绍： Water, Air, & Soil Pollution is an international, interdisciplinary journal on all aspects of pollution and solutions to pollution in the biosphere. This includes chemical, physical and biological processes affecting flora, fauna, water, air and soil in relation to environmental pollution. Because of its scope, the subject areas are diverse and include all aspects of pollution sources, transport, deposition, accumulation, acid precipitation, atmospheric pollution, metals, aquatic pollution including marine pollution and ground water, waste water, pesticides, soil pollution, sewage, sediment pollution, forestry pollution, effects of pollutants on humans, vegetation, fish, aquatic species, micro-organisms, and animals, environmental and molecular toxicology applied to pollution research, biosensors, global and climate change, ecological implications of pollution and pollution models. Water, Air, & Soil Pollution also publishes manuscripts on novel methods used in the study of environmental pollutants, environmental toxicology, environmental biology, novel environmental engineering related to pollution, biodiversity as influenced by pollution, novel environmental biotechnology as applied to pollution (e.g. bioremediation), environmental modelling and biorestoration of polluted environments. Articles should not be submitted that are of local interest only and do not advance international knowledge in environmental pollution and solutions to pollution. Articles that simply replicate known knowledge or techniques while researching a local pollution problem will normally be rejected without review. Submitted articles must have up-to-date references, employ the correct experimental replication and statistical analysis, where needed and contain a significant contribution to new knowledge. The publishing and editorial team sincerely appreciate your cooperation. Water, Air, & Soil Pollution publishes research papers; review articles; mini-reviews; and book reviews.