巯基等离子体聚合涂层与纤维连接蛋白固定相结合增强PCL纳米纤维的生物响应性

IF 7.5 Q1 CHEMISTRY, PHYSICAL
Pegah Zahedifar, Rino Morent, Sheida Aliakbarshirazi, Rouba Ghobeira, Nathalie De Geyter
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引用次数: 0

摘要

为了增强细胞与组织工程中使用的支架的相互作用,支架的形态应该模拟细胞外基质(ECM),并且支架应该具有适当的体积和表面特性。本研究以1-丙硫醇为单体,采用介质阻挡放电(DBD)等离子体在中压下聚合制备富硫醇涂层,以提高疏水静电纺聚己内酯(PCL)纳米纤维的物理化学和生物响应性能。系统研究了载气类型、DBD室压力、处理时间和安田参数(前驱体流量和放电功率的组合)对等离子体聚合过程的影响。通过扫描电子显微镜(SEM)、水接触角(WCA)测量和x射线光电子能谱(XPS)对涂层纳米纤维进行分析,确定了最佳沉积条件,目的是在保持纳米纤维形态的同时获得最高的硫醇密度涂层。我们的研究结果表明,与使用氦气作为载气相比,使用氩气作为载气导致涂层更厚,硫含量明显更高。将沉积时间从5分钟增加到15分钟,最初会增加涂层的厚度、亲水性和硫含量,10分钟后达到饱和点。实验结果表明,最优压力为10kpa,较高的压力会导致纳米纤维熔化。Yasuda参数分析表明,72 MJ/kg的中间值可以提供最佳的硫醇掺入和涂层稳定性。在研究的最后一步,评估了巯基涂层和随后的纤维连接蛋白固定在增强雪旺细胞粘附和增殖方面的有效性。巯基包被的底物表现出优越的蛋白固定化和显著改善的细胞反应。纤维连接蛋白固定后,这些底物表现出最高的细胞活力,粘附和增殖。这些结果强调了巯基等离子体聚合和纤维连接蛋白固定化在促进PCL纳米纤维细胞反应中的协同作用,强调了它们作为组织工程应用的表面修饰策略的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Enhancement of the Bio-Responsiveness of PCL Nanofibers via the Combination of a Thiol Plasma-Polymerized Coating and Fibronectin Immobilization
To enhance cellular interactions with scaffolds used in tissue engineering, scaffold morphology should mimic the extracellular matrix (ECM) and the scaffold should possess appropriate bulk and surface properties. This study aimed to improve the physiochemical and bio-responsive properties of hydrophobic electrospun polycaprolactone (PCL) nanofibers by applying a thiol-rich coating using dielectric barrier discharge (DBD) plasma polymerization at medium pressures with 1-propanethiol as monomer. The effects of carrier gas type, DBD chamber pressure, treatment time, and the Yasuda parameter (a combination of precursor flow rate and discharge power) on the plasma polymerization process were systematically investigated. Optimal deposition conditions were determined by analyzing the coated nanofibers using scanning electron microscopy (SEM), water contact angle (WCA) measurements, and X-ray photoelectron spectroscopy (XPS) aiming to achieve the highest thiol density coating while preserving the nanofibrous morphology. Our findings indicated that using argon as carrier gas resulted in a thicker coating with a significantly higher sulphur content compared to when helium was used as the carrier gas. Increasing deposition time from 5 to 15 minutes initially increased coating thickness, hydrophilicity, and sulphur content, reaching a saturation point after 10 minutes. The optimal chamber pressure was observed to be 10 kPa, as higher pressures caused nanofiber melting. Yasuda parameter analysis revealed that an intermediate value of 72 MJ/kg provided optimal thiol incorporation and coating stability. In a final step of the study, the effectiveness of the thiol coatings and subsequent fibronectin immobilization in enhancing Schwann cell adhesion and proliferation was assessed. The thiol-coated substrates demonstrated superior protein immobilization and significantly improved cell responses. Post-fibronectin immobilization, these substrates exhibited the highest cell viability, adhesion, and proliferation. These results highlight the synergistic effect of thiol plasma polymerization and fibronectin immobilization in promoting cellular responses on PCL nanofibers, underscoring their potential as a surface modification strategy for tissue engineering applications.
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CiteScore
8.10
自引率
1.60%
发文量
128
审稿时长
66 days
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