转录组学和代谢组学分析揭示了根区温度调控柳枝稷幼苗生长节律的潜在机制

IF 6.2 1区 农林科学 Q1 AGRICULTURAL ENGINEERING
Baolin Wu , Junpeng Zuo , Haoyuan Niu , Ruyi Jia , Qi Li , Xiaohong Chen , Zhiquan Yang , Chao Zhang , Fengli Sun , Yajun Xi
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引用次数: 0

摘要

柳枝稷(Panicum virgatum L.)是一种多年生暖季C₄植物,是牧草生产、水土保持和可再生能源的重要资源。然而,其幼苗生长缓慢限制了其更广泛的应用,而这种生长缓慢的潜在原因尚不清楚。本研究研究了模拟根区温度条件下阿拉莫品种幼苗的生长模式、生理指标、转录组学和代谢特征。适度提高根区温度可以促进幼苗生长,表现为株高、茎粗、根长和鲜重的增加。根区温度升高6°C,叶片中的叶绿素、淀粉、木质素、可溶性糖和可溶性蛋白质显著增加,根系活力也随之增加。综合多组学分析显示,与IAA、ABA、JA、BR、SA、ETH和CTK信号通路相关的基因存在差异表达。此外,激素含量在不同温度处理组之间存在差异。值得注意的是,参与细胞分裂素生物合成的ZOG基因被下调,而与双组分系统相关的21个基因持续上调。此外,类黄酮代谢及其生物合成相关基因的表达被确定为响应根区温度变化的关键途径。值得注意的是,随着根区温度的升高,叶片光合作用的增强与根系能量代谢的减弱可能代表了幼苗对根区温度变化的一种平衡机制。这一现象需要进一步调查。我们提出了一个分子模型来解释根区温度变化对柳枝稷幼苗生长节律的影响。这些发现为阐明柳枝稷幼苗发育缓慢的分子机制提供了参考,并为柳枝稷和其他作物的遗传改良提供了有价值的见解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Transcriptome and metabolome analyses reveal the potential mechanism of root zone temperature regulates the growth rhythm of switchgrass (Panicum virgatum L.) seedlings
Switchgrass (Panicum virgatum L.) is a perennial warm-season C₄ plant that is a vital resource for forage production, soil and water conservation, and renewable energy. However, its slow seedling growth limits its broader application, with the underlying reasons for this slow growth not being well understood. This study examined the growth patterns, physiological metrics, and transcriptomic and metabolic profiles of Alamo cultivar seedlings under simulated root-zone temperature conditions. A moderate increase in root-zone temperature could enhance seedling growth, as evidenced by increases in plant height, stem diameter, root length, and fresh weight. A 6 °C increase in root-zone temperature significantly elevated chlorophyll, starch, lignin, soluble sugars, and soluble proteins in leaves, along with root vitality. An integrated multi-omics analysis revealed that genes associated with the IAA, ABA, JA, BR, SA, ETH, and CTK signaling pathways were differentially expressed. Additionally, the hormone content varied across different temperature treatment groups. Notably, the ZOG gene, involved in cytokinin biosynthesis, was downregulated, whereas 21 genes related to the two-component system were consistently upregulated. Additionally, flavonoid metabolism and the expression of genes associated with their biosynthesis were identified as crucial pathways in response to changes in root-zone temperature. Notably, as root zone temperatures increase, enhanced leaf photosynthesis coupled with weakened root energy metabolism may represent a balancing mechanism by which seedlings respond to changes in root zone temperature. This phenomenon requires further investigation. We propose a molecular model to account for the growth rhythm of switchgrass seedlings induced by root zone temperature variations. These findings offer a reference for elucidating the molecular mechanisms underlying the slow development of switchgrass seedlings and offer valuable insights for the genetic improvement of switchgrass and other crops.
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来源期刊
Industrial Crops and Products
Industrial Crops and Products 农林科学-农业工程
CiteScore
9.50
自引率
8.50%
发文量
1518
审稿时长
43 days
期刊介绍: Industrial Crops and Products is an International Journal publishing academic and industrial research on industrial (defined as non-food/non-feed) crops and products. Papers concern both crop-oriented and bio-based materials from crops-oriented research, and should be of interest to an international audience, hypothesis driven, and where comparisons are made statistics performed.
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