顺式-3-己烯醛在多个水平上调节3-羟基-3-甲基戊二酰辅酶a还原酶,促进蒙古黄芪根培养中三萜的产生

IF 6.2 1区 农林科学 Q1 AGRICULTURAL ENGINEERING
Haifeng Sun , Zhenghao Gao , Jinni Zhang , Jinping Jia , Zhi Chai , Wei Ma
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引用次数: 0

摘要

3-羟基-3-甲基戊二酰辅酶a还原酶(HMGR)催化甲羟戊酸(MVA)途径的限速步骤,用于胞浆生物合成类异戊二烯,如三萜和甾醇。本研究旨在探讨顺式-3-己烯醛是否以及如何在蒙古黄芪根培养物中调节HMGR。除顺-3-己烯醛外,还应用了一种HMGR抑制剂洛伐他汀。与对照相比,顺式-3-己烯醛同样使HMGR活性降低到与洛伐他汀相同的水平,但使黄芪甲苷Ⅰ、Ⅲ、环黄芪甲苷Ⅱ和环黄芪醇的含量高于洛伐他汀。有趣的是,顺式-3-己烯醛和洛伐他汀联合使用可使黄芪甲苷Ⅰ、Ⅱ、Ⅲ和环头甲苷Ⅱ的含量在24 小时内显著提高。通过分子对接、体外培养和测定HMGR水平,表明HMGR活性降低不是由于顺-3-己烯醛的抑制。转录组分析显示,顺式-3-己烯醛显著增加了编码HMGR和甾醇C-22去饱和酶的四个转录本的丰度。进一步的实时PCR和相关分析表明,由isoform_35315编码的SNF5是参与顺式-3-己烯醛及其与洛伐他汀联用对HMGR多水平调控的关键转录调控蛋白,并具有同型特异性和时间依赖性。综上所述,我们的研究结果阐明了顺式-3-己烯醛和洛伐他汀促进蒙古黄芪根培养中黄芪甲苷和环黄芪醇生成的调节机制和潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Cis-3-hexenal modulates 3-hydroxy-3-methylglutaryl-CoA reductase at multiple levels to enhance triterpenoid production in the root cultures of Astragalus mongholicus Bunge
3-Hydroxy-3-methylglutaryl-CoA reductase (HMGR) catalyzes the rate-limiting step of the mevalonate (MVA) pathway for cytosolic biosynthesis of isoprenoids such as triterpenoids and sterols. The current study aimed to investigate whether and how HMGR was modulated by cis-3-hexenal in this process in A. mongholicus root cultures. In addition to cis-3-hexenal, lovastatin, an inhibitor of HMGR, was also applied. Compared with the control, cis-3-hexenal similarly reduced HMGR activity to the same level as lovastatin but increased the contents of astragaloside Ⅰ, Ⅲ, cyclocephaloside Ⅱ, and cycloastragenol to higher levels than those of lovastatin. Interestingly, the combination of cis-3-hexenal and lovastatin enhanced the contents of astragaloside Ⅰ, Ⅱ, Ⅲ, and cyclocephaloside Ⅱ to much higher levels within 24 hours. This result revealed that the reduced HMGR activity was not due to inhibition by cis-3-hexenal, which was confirmed by molecular docking, in vitro incubation, and measuring HMGR level. Transcriptome analysis revealed that cis-3-hexenal significantly increased the abundance of four transcripts encoding HMGR and sterol C-22 desaturase. Further real-time PCR and correlation analysis indicated that SNF5, encoded by isoform_35315, was a key transcription regulatory protein involved in multilevel control of HMGR by cis-3-hexenal and its combination with lovastatin in an isoform-specific and time-dependent pattern. In summary, our results elucidated the modulating mechanism and potential of cis-3-hexenal and lovastatin in enhancing astragalosides and cycloastragenol production in A. mongholicus root cultures.
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来源期刊
Industrial Crops and Products
Industrial Crops and Products 农林科学-农业工程
CiteScore
9.50
自引率
8.50%
发文量
1518
审稿时长
43 days
期刊介绍: Industrial Crops and Products is an International Journal publishing academic and industrial research on industrial (defined as non-food/non-feed) crops and products. Papers concern both crop-oriented and bio-based materials from crops-oriented research, and should be of interest to an international audience, hypothesis driven, and where comparisons are made statistics performed.
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