nir响应工程胶体聚苯胺/ZnO/UiO-66-NH₂纳米平台:用于乳腺癌精密治疗的多功能光热剂

IF 6.2 2区 工程技术 Q1 ENGINEERING, MULTIDISCIPLINARY
Shefa Mirani Nezhad , Aboutaleb Kousha , Yasser Rajabi , Ehsan Nazarzadeh Zare , Saeed Rahimi
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引用次数: 0

摘要

分两步构建了聚苯胺/ZnO/UiO-66-NH 2 (PANI/ZnO/UiO-66-NH 2)纳米平台。该纳米平台在近红外(NIR)区域表现出较强的光吸收,能够在808 nm激光照射下将光能有效转化为热能,从而促进肿瘤细胞的消融。采用FT-IR, EDX, XRD, FESEM, UV-Vis和TG进行表征。生物活性,包括抗氧化和抗菌性能,以及细胞毒性进行了评估。该纳米复合材料具有很高的光热效率,在808 nm近红外激光(0.6 W/cm²,1.5 mg/mL)下,在10 min内达到60°C,证实了其在高温癌症治疗中的潜力。在0.15 mg/mL时,温度升高到49℃,证明了其浓度和激光功率对有效光热治疗(PTT)的依赖性。对MCF-7乳腺癌细胞的体外细胞毒性分析显示,近红外激光照射显著降低了细胞活力。MTT试验证实,将纳米复合材料与激光治疗相结合可显著降低癌细胞存活率,凸显了其作为光热剂的潜力。此外,发现胶体PANI/ZnO/UiO-66-NH 2纳米复合材料的抗氧化活性为90.64 %,与UiO-66-NH 2的最高效率(94.20 %)相当,并且对大肠杆菌(E. coli),枯草芽孢杆菌(B. subtilis),肠炎沙门氏菌(S. enteritidis)和金黄色葡萄球菌(S. aureus)具有较强的抗菌作用。对肠炎沙门氏菌和大肠杆菌的抑制带直径分别为15 ± 0.3 mm和13 ± 0.1 mm,具有PTT和抗菌治疗的双重适用性。MTT实验显示,暴露48 h后,胶体PANI/ZnO/ uio -66- nh2使MCF-7细胞的活力降低到40% %。抑制MCF-7细胞生长的胶体PANI/ZnO/ uui -66- nh2的IC₅₀值在24 h后从17.13 µg/mL增加到48 h后的32.3 µg/mL。此外,溶血测试表明,纳米平台在浓度为100 μg/mL时溶血率为5 %。这些结果突出了纳米复合材料在生物医学上的广泛潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
NIR-responsive engineered colloidal polyaniline/ZnO/UiO-66-NH₂ nanoplatform: A multifunctional photothermal agent for precision therapy in breast cancer
A colloidal polyaniline/ZnO/UiO-66-NH₂ (PANI/ZnO/UiO-66-NH₂) nano-platform was constructed in two steps. The nano-platform exhibited strong optical absorption in the near-infrared (NIR) region, enabling efficient conversion of optical energy into thermal energy under 808-nm laser irradiation, thereby facilitating tumor cell ablation. Characterization was performed using FT-IR, EDX, XRD, FESEM, UV-Vis, and TG. Biological activities, including antioxidant and antibacterial properties, as well as cytotoxicity, were evaluated. The nanocomposite exhibited high photothermal efficiency, reaching 60 °C in 10 min under an 808 nm NIR laser (0.6 W/cm², 1.5 mg/mL), confirming its potential for hyperthermia-based cancer therapy. At 0.15 mg/mL, the temperature rose to 49 °C, demonstrating its concentration and laser power dependence for effective photothermal therapy (PTT). In vitro cytotoxicity analysis on MCF-7 breast cancer cells showed a significant reduction in cell viability under NIR laser irradiation. The MTT assay confirmed that combining the nanocomposite with laser treatment led to a substantial decrease in cancer cell survival, highlighting its potential as a photothermal agent. Additionally, the antioxidant activity of the colloidal PANI/ZnO/UiO-66-NH₂ nanocomposite was found to be 90.64 %, comparable to the highest observed efficiency of UiO-66-NH₂ (94.20 %), and potent antibacterial effects against Escherichia coli (E. coli), Bacillus subtilis (B. subtilis), Salmonella enteritidis (S. enteritidis), and Staphylococcus aureus (S. aureus). Inhibition zone diameters for S. enteritidis and E. coli were 15 ± 0.3 mm and 13 ± 0.1 mm, respectively, underscoring dual applicability in PTT and antibacterial therapy. The MTT assay revealed that colloidal PANI/ZnO/UiO-66-NH₂ reduced the viability of MCF-7 cells to 40 % after 48 h of exposure. The IC₅₀ value of the colloidal PANI/ZnO/UiO-66-NH₂ for inhibiting the growth of MCF-7 cells increased from 17.13 µg/mL after 24 h to 32.3 µg/mL after 48 h. Additionally, hemolysis testing demonstrated that the nano-platform exhibited a hemolysis rate of 5 % at a concentration of 100 μg/mL. These results highlight the nanocomposite’s broad biomedical potential.
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来源期刊
alexandria engineering journal
alexandria engineering journal Engineering-General Engineering
CiteScore
11.20
自引率
4.40%
发文量
1015
审稿时长
43 days
期刊介绍: Alexandria Engineering Journal is an international journal devoted to publishing high quality papers in the field of engineering and applied science. Alexandria Engineering Journal is cited in the Engineering Information Services (EIS) and the Chemical Abstracts (CA). The papers published in Alexandria Engineering Journal are grouped into five sections, according to the following classification: • Mechanical, Production, Marine and Textile Engineering • Electrical Engineering, Computer Science and Nuclear Engineering • Civil and Architecture Engineering • Chemical Engineering and Applied Sciences • Environmental Engineering
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