基于episisal载体的CRISPR/Cas9系统生成MYL3敲除干细胞系(WAe009-A-1H

IF 0.8 4区 医学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Rui Bai , Wei Fu , Xiaojie Hou , Juyi Wan
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引用次数: 0

摘要

肌球蛋白轻链3 (MYL3)基因编码心室必需轻链异构体,该异构体是肌球蛋白过桥动力学的重要调节剂。MYL3的变异是肥厚性心肌病和扩张性心肌病合并心力衰竭和心源性猝死(SCD)的原因。为了进一步阐明MYL3在心肌病发病机制中的作用,我们利用CRISPR/Cas9系统创建了MYL3敲除的人胚胎干细胞系。值得注意的是,这种myl3敲除细胞系保持了正常的形态、多能性和核型。该资源为调查与myl3相关的健康和疾病提供了有价值的工具。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Generation of a MYL3 knockout stem cell line (WAe009-A-1H) by episomal vector-based CRISPR/Cas9 system
The Myosin light chain 3 (MYL3) gene encodes the ventricular essential light chain isoform, which is an important modulator of sarcomeric myosin cross-bridge kinetics. Variants in MYL3 are a cause of hypertrophic cardiomyopathy and dilated cardiomyopathy with cardiac failure and sudden cardiac death (SCD). To further elucidate the involvement of MYL3 in the pathogenesis of cardiomyopathies, we have created a MYL3 knockout human embryonic stem cell line using the CRISPR/Cas9 system. Notably, this MYL3-knockout cell line retains normal morphology, pluripotency, and karyotype. This resource provides a valuable tool for investigating MYL3-related health and disease.
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来源期刊
Stem cell research
Stem cell research 生物-生物工程与应用微生物
CiteScore
2.20
自引率
8.30%
发文量
338
审稿时长
55 days
期刊介绍: Stem Cell Research is dedicated to publishing high-quality manuscripts focusing on the biology and applications of stem cell research. Submissions to Stem Cell Research, may cover all aspects of stem cells, including embryonic stem cells, tissue-specific stem cells, cancer stem cells, developmental studies, stem cell genomes, and translational research. Stem Cell Research publishes 6 issues a year.
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