用金修饰的纳米吸管制造可重复的、可逆的和高信号变化的适配体传感器

IF 8.2 2区 材料科学 Q1 MATERIALS SCIENCE, MULTIDISCIPLINARY
Ana B. Ramirez,  and , Robert A. Lazenby*, 
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引用次数: 0

摘要

适配体功能化纳米吸管是一类新兴的生物传感器,用于特定分子的无标记检测。虽然存在各种将单链DNA适配体固定到玻璃纳米吸管内壁上的策略,但制造方法对传感器灵敏度、信号变化、再现性和可靠性的影响仍未得到探索。在这项研究中,我们比较了三种制造方法,发现使用纳米吸管内合成的金纳米颗粒(AuNPs)制造的传感器产生了最可重复的结果,同时也允许控制修饰过程。相比之下,其他两种适配体固定方法依赖于胺化或硫化适配体偶联的多步聚合物涂层,但由于水敏感性和聚合物沉积不均匀,导致传感器响应不一致。使用aunp包覆的纳米吸管,我们成功地制造了许多不同尺寸的传感器,证明了较小的纳米吸管产生更大的信号变化。使用直径从22到30 nm的玻璃纳米吸管构建的传感器显示,当AuNP合成在尖端开口附近产生颗粒而不造成堵塞时,信号变化很大(>40%)。然而,我们也观察到传感器的信号变化明显较低(使用相同尺寸的玻璃纳米吸管),我们将其归因于尖端存在的最小Au或相反,当Au明显阻塞探针时。这些结果强调了制造方法在最大化信号变化,增强再现性以及识别传感器失效的方式和原因方面的关键作用。这项工作旨在促进在分析传感应用中更广泛地采用适配体功能化纳米吸管。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Fabricating Reproducible, Reversible, and High Signal Change Aptasensors with Gold-Modified Nanopipettes

Fabricating Reproducible, Reversible, and High Signal Change Aptasensors with Gold-Modified Nanopipettes

Aptamer-functionalized nanopipettes are an emerging class of biosensors for the label-free detection of specific molecules. While various strategies exist for immobilizing single-stranded DNA aptamers onto the inner walls of glass nanopipettes, the impact of the fabrication method on sensor sensitivity, signal change, reproducibility, and reliability remains unexplored. In this study, we compared three fabrication methods and found that sensors fabricated using gold nanoparticles (AuNPs) synthesized within the nanopipettes produced the most reproducible results while also allowing control over the modification process. In contrast, two other aptamer immobilization methods, which relied on multistep polymer coatings with aminated or thiolated aptamer coupling, were hindered by water sensitivity and uneven polymer deposition, resulting in inconsistent sensor responses. Using the AuNP-coated nanopipettes, we successfully fabricated numerous sensors of varying sizes, demonstrating that smaller nanopipettes produce greater signal changes. Sensors constructed using glass nanopipettes with diameters ranging from 22 to 30 nm exhibited large signal changes (>40%) when AuNP synthesis produced particles near the tip opening without causing blockage. However, we also observed sensors with signal changes that were significantly lower (using the same-sized glass nanopipettes), which we attributed to either minimal Au present at the tip or conversely when Au significantly blocked the probe. These results highlight the critical role of fabrication methods in maximizing the signal change, enhancing the reproducibility, and identifying how and why sensors fail. This work aims to facilitate the broader adoption of aptamer-functionalized nanopipettes in analytical sensing applications.

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来源期刊
ACS Applied Materials & Interfaces
ACS Applied Materials & Interfaces 工程技术-材料科学:综合
CiteScore
16.00
自引率
6.30%
发文量
4978
审稿时长
1.8 months
期刊介绍: ACS Applied Materials & Interfaces is a leading interdisciplinary journal that brings together chemists, engineers, physicists, and biologists to explore the development and utilization of newly-discovered materials and interfacial processes for specific applications. Our journal has experienced remarkable growth since its establishment in 2009, both in terms of the number of articles published and the impact of the research showcased. We are proud to foster a truly global community, with the majority of published articles originating from outside the United States, reflecting the rapid growth of applied research worldwide.
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