基于NH2-GO/Fe-Ni@Ti0033C2Tx/NH2-GO/Fc@AuNPs和BPNPs/AuPt-MB双信号放大的三明治结构比例电化学免疫传感器检测survivin

IF 8 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical Pub Date : 2025-04-28 DOI:10.1016/j.snb.2025.137882

Zhiren Zhou , Lina Zou , Ping Zhang , Chunmei Li , Ye Zhu , Huanyu Ren , Zheng Li , Huiru Niu , Hao Liao , Xiaojing Zhang , Shanshan An , Fei Ren , Xiuhong Ge , Lang Cheng , Feiyan Yang , Hongzhi Pan , Shengzhong Rong , Dong Chang , Hongkun Ma

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引用次数: 0

摘要

Survivin作为膀胱癌的重要生物标志物，探索其检测方法具有重要意义。为此，研制了一种灵敏的比例电化学免疫传感器。简而言之，Fe-Ni@Ti3C2Tx （NH2-GO/Fe-Ni@Ti3C2Tx/NH2-GO）增强的双层氨基氧化石墨烯（NH2-GO）夹层显著放大了检测信号。ferrocene@Au纳米粒子（Fc@AuNPs）提供了参考信号，Ab1通过Au-N键锚定。此外，aupt -亚甲基蓝（AuPt-MB）不仅作为次级信号，还通过Au-NH2和Pt-NH2键锚定Ab2。黑磷纳米板（BPNPs）提高了电导率，并为AuPt-MB提供了额外的结合位点。该免疫传感器具有较宽的检测范围（0.001~1000 ng/mL）和较低的检出限（0.00065 ng/mL），可成功检测人类尿液样本中的survivin，具有良好的临床应用前景。

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A sandwich-configuration ratiometric electrochemical immunosensor based on dual signal amplification of NH2-GO/Fe-Ni@Ti0033C2Tx/NH2-GO/Fc@AuNPs and BPNPs/AuPt-MB for the detection of survivin

Survivin serves as a crucial biomarker for bladder cancer, so exploring the detection method is essential. Therefore, a sensitive ratiometric electrochemical immunosensor was developed. In brief, a double-layered amino graphene oxide (NH₂-GO) sandwich enhanced by Fe-Ni@Ti₃C₂T_x (NH₂-GO/Fe-Ni@Ti₃C₂T_x/NH₂-GO) significantly amplified the detection signal. ferrocene@Au nanoparticles (Fc@AuNPs) provided a reference signal, and Ab₁ was anchored via an Au-N bond. Moreover, AuPt-methylene blue (AuPt-MB) served not only as a secondary signal but also anchored Ab₂ through Au-NH₂ and Pt-NH₂ bonds. Black phosphorus nanoplates (BPNPs) improved conductivity and provided additional binding sites for AuPt-MB. The immunosensor exhibited a broad detection range (0.001–1000 ng/mL) and a low detection limit (0.00065 ng/mL), facilitating successful survivin detection in human urine samples with promising clinical analysis applications.

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来源期刊

Sensors and Actuators B: Chemical 工程技术-电化学

CiteScore

14.60

自引率

11.90%

发文量

1776

审稿时长

3.2 months

期刊介绍： Sensors & Actuators, B: Chemical is an international journal focused on the research and development of chemical transducers. It covers chemical sensors and biosensors, chemical actuators, and analytical microsystems. The journal is interdisciplinary, aiming to publish original works showcasing substantial advancements beyond the current state of the art in these fields, with practical applicability to solving meaningful analytical problems. Review articles are accepted by invitation from an Editor of the journal.