Monier Alhadi Abdelrahman Mohammed, Zhenbin Chen, Xueyan Du, Bin Li, Jinhan Zhang, Tai Kang
{"title":"聚己二酸丁二酯/ -环糊精/聚乙烯醇掺杂Mg2+包被再生Fe3O4颗粒载体提高固定化青霉素G酰化酶性能的研究","authors":"Monier Alhadi Abdelrahman Mohammed, Zhenbin Chen, Xueyan Du, Bin Li, Jinhan Zhang, Tai Kang","doi":"10.1007/s00396-025-05391-x","DOIUrl":null,"url":null,"abstract":"<div><p>In this study, Fe<sub>3</sub>O<sub>4</sub> magnetic particles (MPs) were produced from nickel slag using molten oxidation and magnetic separation methods. After that, Fe<sub>3</sub>O<sub>4</sub> MPs were coated with a blend of poly (butylene adipate-co-terephthalate)/beta-cyclodextrin/polyvinyl alcohol (PBAT/B-CD/PVA), and Mg<sup>2+</sup> was doped during this process. Mg<sup>2+</sup>-Fe<sub>3</sub>O<sub>4</sub>@PBAT/B-CD/PVA MPs were then modified using γ-glycidoxypropyltrimethoxysilane (KH560) to produce Mg<sup>2+</sup>-Fe<sub>3</sub>O<sub>4</sub>@PBAT/B-CD/PVA-g-KH560 MPs. The immobilized PGA (Mg<sup>2+</sup>-Fe<sub>3</sub>O<sub>4</sub>@PBAT/B-CD/PVA-g-KH560-PGA MPs) was obtained through covalently bonding PGA under mild circumstances by a click reaction between the amino group of PGA and the epoxy group of KH560. Furthermore, a vibrating sample magnetometer (VSM), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning electron microscopy-energy spectroscopy of dispersive x-rays (SEM–EDS), X-ray photoelectron spectrophotometry (XPS), and inductively coupled plasma mass spectrometry (ICP-MS) were used to characterize the magnetic composite structures. The results showed that the immobilized PGA had values of 31,574 U/g, 94.2%, and 113 mg/g for enzyme activity recovery (<i>EAR</i>), enzyme loading capacity (<i>ELC</i>), and enzyme activity (<i>EA</i>), respectively. Its enzyme activity was maintained at 74% after 12 cycles of usage. The Fe<sub>3</sub>O<sub>4</sub>@PBAT/B-CD/PVA MPs were prepared similarly to the preparation of Mg<sup>2+</sup>-Fe<sub>3</sub>O<sub>4</sub>@PBAT/b-CD/PVA MPs except that Mg<sup>2+</sup> was not doped to it to adopt as control, and after 6 cycles of usage, the immobilized PGA (Fe<sub>3</sub>O<sub>4</sub>@PBAT/B-CD/PVA-g-KH560-PGA MPs) retained 50% of its original enzyme activity, with <i>EA</i>, <i>EAR</i>, and <i>ELC</i> values of 28,513 U/g, 85.2%, and 107 mg/g, respectively, which were lower than Mg<sup>2+</sup>-Fe<sub>3</sub>O<sub>4</sub>@PBAT/B-CD/PVA-g-KH560 MPs; this indicate that doped Mg<sup>2+</sup> had positive impact on immobilized PGA.</p><h3>Graphical Abstract</h3><p>Preparation scheme of immobilized PGA (Mg<sup>+2</sup>-Fe<sub>3</sub>O<sub>4</sub>@PBAT/B-CD/PVA-g-KH560-PGA MPs)</p><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":520,"journal":{"name":"Colloid and Polymer Science","volume":"303 5","pages":"937 - 957"},"PeriodicalIF":2.2000,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Study of poly (butylene adipate-co-terephthalate)/beta-cyclodextrin/polyvinyl alcohol, doped Mg2+, coated recycled Fe3O4 particle carriers for enhancing immobilization penicillin G acylase performance\",\"authors\":\"Monier Alhadi Abdelrahman Mohammed, Zhenbin Chen, Xueyan Du, Bin Li, Jinhan Zhang, Tai Kang\",\"doi\":\"10.1007/s00396-025-05391-x\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>In this study, Fe<sub>3</sub>O<sub>4</sub> magnetic particles (MPs) were produced from nickel slag using molten oxidation and magnetic separation methods. After that, Fe<sub>3</sub>O<sub>4</sub> MPs were coated with a blend of poly (butylene adipate-co-terephthalate)/beta-cyclodextrin/polyvinyl alcohol (PBAT/B-CD/PVA), and Mg<sup>2+</sup> was doped during this process. Mg<sup>2+</sup>-Fe<sub>3</sub>O<sub>4</sub>@PBAT/B-CD/PVA MPs were then modified using γ-glycidoxypropyltrimethoxysilane (KH560) to produce Mg<sup>2+</sup>-Fe<sub>3</sub>O<sub>4</sub>@PBAT/B-CD/PVA-g-KH560 MPs. The immobilized PGA (Mg<sup>2+</sup>-Fe<sub>3</sub>O<sub>4</sub>@PBAT/B-CD/PVA-g-KH560-PGA MPs) was obtained through covalently bonding PGA under mild circumstances by a click reaction between the amino group of PGA and the epoxy group of KH560. Furthermore, a vibrating sample magnetometer (VSM), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning electron microscopy-energy spectroscopy of dispersive x-rays (SEM–EDS), X-ray photoelectron spectrophotometry (XPS), and inductively coupled plasma mass spectrometry (ICP-MS) were used to characterize the magnetic composite structures. The results showed that the immobilized PGA had values of 31,574 U/g, 94.2%, and 113 mg/g for enzyme activity recovery (<i>EAR</i>), enzyme loading capacity (<i>ELC</i>), and enzyme activity (<i>EA</i>), respectively. Its enzyme activity was maintained at 74% after 12 cycles of usage. The Fe<sub>3</sub>O<sub>4</sub>@PBAT/B-CD/PVA MPs were prepared similarly to the preparation of Mg<sup>2+</sup>-Fe<sub>3</sub>O<sub>4</sub>@PBAT/b-CD/PVA MPs except that Mg<sup>2+</sup> was not doped to it to adopt as control, and after 6 cycles of usage, the immobilized PGA (Fe<sub>3</sub>O<sub>4</sub>@PBAT/B-CD/PVA-g-KH560-PGA MPs) retained 50% of its original enzyme activity, with <i>EA</i>, <i>EAR</i>, and <i>ELC</i> values of 28,513 U/g, 85.2%, and 107 mg/g, respectively, which were lower than Mg<sup>2+</sup>-Fe<sub>3</sub>O<sub>4</sub>@PBAT/B-CD/PVA-g-KH560 MPs; this indicate that doped Mg<sup>2+</sup> had positive impact on immobilized PGA.</p><h3>Graphical Abstract</h3><p>Preparation scheme of immobilized PGA (Mg<sup>+2</sup>-Fe<sub>3</sub>O<sub>4</sub>@PBAT/B-CD/PVA-g-KH560-PGA MPs)</p><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>\",\"PeriodicalId\":520,\"journal\":{\"name\":\"Colloid and Polymer Science\",\"volume\":\"303 5\",\"pages\":\"937 - 957\"},\"PeriodicalIF\":2.2000,\"publicationDate\":\"2025-02-25\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Colloid and Polymer Science\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s00396-025-05391-x\",\"RegionNum\":4,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"CHEMISTRY, PHYSICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Colloid and Polymer Science","FirstCategoryId":"92","ListUrlMain":"https://link.springer.com/article/10.1007/s00396-025-05391-x","RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"CHEMISTRY, PHYSICAL","Score":null,"Total":0}
Study of poly (butylene adipate-co-terephthalate)/beta-cyclodextrin/polyvinyl alcohol, doped Mg2+, coated recycled Fe3O4 particle carriers for enhancing immobilization penicillin G acylase performance
In this study, Fe3O4 magnetic particles (MPs) were produced from nickel slag using molten oxidation and magnetic separation methods. After that, Fe3O4 MPs were coated with a blend of poly (butylene adipate-co-terephthalate)/beta-cyclodextrin/polyvinyl alcohol (PBAT/B-CD/PVA), and Mg2+ was doped during this process. Mg2+-Fe3O4@PBAT/B-CD/PVA MPs were then modified using γ-glycidoxypropyltrimethoxysilane (KH560) to produce Mg2+-Fe3O4@PBAT/B-CD/PVA-g-KH560 MPs. The immobilized PGA (Mg2+-Fe3O4@PBAT/B-CD/PVA-g-KH560-PGA MPs) was obtained through covalently bonding PGA under mild circumstances by a click reaction between the amino group of PGA and the epoxy group of KH560. Furthermore, a vibrating sample magnetometer (VSM), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning electron microscopy-energy spectroscopy of dispersive x-rays (SEM–EDS), X-ray photoelectron spectrophotometry (XPS), and inductively coupled plasma mass spectrometry (ICP-MS) were used to characterize the magnetic composite structures. The results showed that the immobilized PGA had values of 31,574 U/g, 94.2%, and 113 mg/g for enzyme activity recovery (EAR), enzyme loading capacity (ELC), and enzyme activity (EA), respectively. Its enzyme activity was maintained at 74% after 12 cycles of usage. The Fe3O4@PBAT/B-CD/PVA MPs were prepared similarly to the preparation of Mg2+-Fe3O4@PBAT/b-CD/PVA MPs except that Mg2+ was not doped to it to adopt as control, and after 6 cycles of usage, the immobilized PGA (Fe3O4@PBAT/B-CD/PVA-g-KH560-PGA MPs) retained 50% of its original enzyme activity, with EA, EAR, and ELC values of 28,513 U/g, 85.2%, and 107 mg/g, respectively, which were lower than Mg2+-Fe3O4@PBAT/B-CD/PVA-g-KH560 MPs; this indicate that doped Mg2+ had positive impact on immobilized PGA.
Graphical Abstract
Preparation scheme of immobilized PGA (Mg+2-Fe3O4@PBAT/B-CD/PVA-g-KH560-PGA MPs)
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