一种基于3D打印螺旋混合器和磁连续流分离的比色生物传感器,用于灵敏检测离心管中的沙门氏菌

IF 10.7 1区 生物学 Q1 BIOPHYSICS
Siyi Ye, Kaiyuan Jia, Meixuan Li, Ying Ding, Gang Liu, Jianhan Lin, Yuhe Wang, Lei Wang
{"title":"一种基于3D打印螺旋混合器和磁连续流分离的比色生物传感器,用于灵敏检测离心管中的沙门氏菌","authors":"Siyi Ye,&nbsp;Kaiyuan Jia,&nbsp;Meixuan Li,&nbsp;Ying Ding,&nbsp;Gang Liu,&nbsp;Jianhan Lin,&nbsp;Yuhe Wang,&nbsp;Lei Wang","doi":"10.1016/j.bios.2025.117509","DOIUrl":null,"url":null,"abstract":"<div><div>Early screening of foodborne pathogens is effective to prevent food poisoning. In this work, a portable colorimetric biosensor was innovatively developed on a centrifugal tube using a 3D printed spiral mechanical mixer for rapid immune reaction, a 3D printed magnetic field generator for continuous-flow bacterial capture, Au@Pt nanozymes for effective signal amplification, and a smartphone application for accurate image processing. The rapid and efficient mixing of specific antibodies on the magnetic beads and Au@Pt nanozymes with target bacteria was simply achieved through repeated press-and-release actions on the mixer to produce the vortex turbulence in the sample chamber and thus form the magnetic bead-bacteria-nanozyme conjugates. The air pressure in the centrifuge tube was adjusted by manual operation on the disposable syringe. More importantly, the entire bacterial detection procedure from separation and labelling, then to washing and catalysis, and finally to measurement and analysis, was achieved on a single centrifugal tube. Besides, all the wastes were collected inside the tube to avoid cross-contamination risks. This disposable biosensor was featured with easy operation, low cost and high sensitivity, and was able to detect <em>Salmonella typhimurium</em> as low as 16 CFU/mL in 1 h, which might pave a pathway for on-site screening of foodborne pathogens to ensure food safety.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"282 ","pages":"Article 117509"},"PeriodicalIF":10.7000,"publicationDate":"2025-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"A colorimetric biosensor based on 3D printed spiral mixer and magnetic continuous-flow separation for sensitive detection of Salmonella in centrifuge tubes\",\"authors\":\"Siyi Ye,&nbsp;Kaiyuan Jia,&nbsp;Meixuan Li,&nbsp;Ying Ding,&nbsp;Gang Liu,&nbsp;Jianhan Lin,&nbsp;Yuhe Wang,&nbsp;Lei Wang\",\"doi\":\"10.1016/j.bios.2025.117509\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Early screening of foodborne pathogens is effective to prevent food poisoning. In this work, a portable colorimetric biosensor was innovatively developed on a centrifugal tube using a 3D printed spiral mechanical mixer for rapid immune reaction, a 3D printed magnetic field generator for continuous-flow bacterial capture, Au@Pt nanozymes for effective signal amplification, and a smartphone application for accurate image processing. The rapid and efficient mixing of specific antibodies on the magnetic beads and Au@Pt nanozymes with target bacteria was simply achieved through repeated press-and-release actions on the mixer to produce the vortex turbulence in the sample chamber and thus form the magnetic bead-bacteria-nanozyme conjugates. The air pressure in the centrifuge tube was adjusted by manual operation on the disposable syringe. More importantly, the entire bacterial detection procedure from separation and labelling, then to washing and catalysis, and finally to measurement and analysis, was achieved on a single centrifugal tube. Besides, all the wastes were collected inside the tube to avoid cross-contamination risks. This disposable biosensor was featured with easy operation, low cost and high sensitivity, and was able to detect <em>Salmonella typhimurium</em> as low as 16 CFU/mL in 1 h, which might pave a pathway for on-site screening of foodborne pathogens to ensure food safety.</div></div>\",\"PeriodicalId\":259,\"journal\":{\"name\":\"Biosensors and Bioelectronics\",\"volume\":\"282 \",\"pages\":\"Article 117509\"},\"PeriodicalIF\":10.7000,\"publicationDate\":\"2025-04-26\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biosensors and Bioelectronics\",\"FirstCategoryId\":\"1\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0956566325003835\",\"RegionNum\":1,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"BIOPHYSICS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biosensors and Bioelectronics","FirstCategoryId":"1","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0956566325003835","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOPHYSICS","Score":null,"Total":0}
引用次数: 0

摘要

早期筛查食源性致病菌是预防食物中毒的有效方法。在这项工作中,在离心管上创新地开发了便携式比色生物传感器,使用3D打印螺旋机械混合器进行快速免疫反应,3D打印磁场发生器用于连续流动细菌捕获,Au@Pt纳米酶用于有效的信号放大,以及用于精确图像处理的智能手机应用程序。磁珠上的特异性抗体和Au@Pt纳米酶与目标细菌的快速有效混合,只需在搅拌器上重复按压和释放动作,在样品室中产生涡流,从而形成磁珠-细菌-纳米酶偶联物。在一次性注射器上手动调节离心管内的气压。更重要的是,从分离和标记到洗涤和催化,最后到测量和分析的整个细菌检测过程都是在一个离心管上完成的。此外,所有废物都在管内收集,以避免交叉污染风险。该一次性生物传感器操作简单、成本低、灵敏度高,可在1 h内检测到低至16 CFU/mL的鼠伤寒沙门菌,为现场检测食源性致病菌奠定基础,确保食品安全。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A colorimetric biosensor based on 3D printed spiral mixer and magnetic continuous-flow separation for sensitive detection of Salmonella in centrifuge tubes
Early screening of foodborne pathogens is effective to prevent food poisoning. In this work, a portable colorimetric biosensor was innovatively developed on a centrifugal tube using a 3D printed spiral mechanical mixer for rapid immune reaction, a 3D printed magnetic field generator for continuous-flow bacterial capture, Au@Pt nanozymes for effective signal amplification, and a smartphone application for accurate image processing. The rapid and efficient mixing of specific antibodies on the magnetic beads and Au@Pt nanozymes with target bacteria was simply achieved through repeated press-and-release actions on the mixer to produce the vortex turbulence in the sample chamber and thus form the magnetic bead-bacteria-nanozyme conjugates. The air pressure in the centrifuge tube was adjusted by manual operation on the disposable syringe. More importantly, the entire bacterial detection procedure from separation and labelling, then to washing and catalysis, and finally to measurement and analysis, was achieved on a single centrifugal tube. Besides, all the wastes were collected inside the tube to avoid cross-contamination risks. This disposable biosensor was featured with easy operation, low cost and high sensitivity, and was able to detect Salmonella typhimurium as low as 16 CFU/mL in 1 h, which might pave a pathway for on-site screening of foodborne pathogens to ensure food safety.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Biosensors and Bioelectronics
Biosensors and Bioelectronics 工程技术-电化学
CiteScore
20.80
自引率
7.10%
发文量
1006
审稿时长
29 days
期刊介绍: Biosensors & Bioelectronics, along with its open access companion journal Biosensors & Bioelectronics: X, is the leading international publication in the field of biosensors and bioelectronics. It covers research, design, development, and application of biosensors, which are analytical devices incorporating biological materials with physicochemical transducers. These devices, including sensors, DNA chips, electronic noses, and lab-on-a-chip, produce digital signals proportional to specific analytes. Examples include immunosensors and enzyme-based biosensors, applied in various fields such as medicine, environmental monitoring, and food industry. The journal also focuses on molecular and supramolecular structures for enhancing device performance.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信