人内源性逆转录病毒在慢性静脉疾病患者中的表达及其与炎症状态的相互作用

IF 0.5 Q4 GENETICS & HEREDITY

Human Gene Pub Date : 2025-04-25 DOI:10.1016/j.humgen.2025.201411

Kevin Cézar Nascimento Silva , Ana Paula Augusto da Cruz Ballerini , Bruna Reimberg Flose , Michelly Damasceno Silva , Célia Aparecida Marques Pimenta , Marina Tiemi Shio , Jonatas Bussador do Amaral , Liã Bárbara Arruda , André Luis Lacerda Bachi , Carolina Nunes França , Luiz Henrique da Silva Nali

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引用次数: 0

摘要

慢性静脉疾病（CVD）包括几种以慢性炎症状态为特征的静脉疾病，导致严重的血液循环障碍。虽然人类内源性逆转录病毒（herv）在自身免疫性疾病和炎症反应中发挥作用，但其对心血管疾病的影响尚不清楚。在这里，我们描述了herv的表达水平和CVD患者的全身炎症状态。方法共纳入85例志愿者，其中心血管疾病组39例，对照组46例。采用Real Time PCR检测HERV-W-env和HERV-K-gag的表达，以GAPDH作为管家基因推断相对定量。ELISA法测定血清细胞因子IL-6、IL-10、TNF-α水平。结果CVD患者sherv - w -env表达水平是对照组的6倍(p <；0.01)，而HERV-K-gag在CVD患者中不表达。CVD患者血清中促炎因子IL-6、TNF-α浓度高于对照组(p <；0.01)。CVD患者IL-10与TNF-α、IL-6、HERV-W、TNF-α/ IL-10呈正相关（p < 0.01）。结论研究结果表明HERV-W在CVD中具有较高的转录活性，提示HERV-W可能参与CVD的发病机制。

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Profile of expression of human endogenous retroviruses and their interplay on inflammatory status in patients with chronic venous disease

Introduction

Chronic Venous Disease (CVD) encompass several venous disorders characterized by chronic inflammatory state leading to sever impairment of blood return. Although Human Endogenous Retroviruses (HERVs) have shown a role in autoimmune diseases and inflammatory responses, its impact on CVD is poorly understood. Here we described the level of HERVs expression and the systemic inflammatory status in CVD patients.

Methods

A total of 85 volunteers (CVD n = 39, control group n = 46) were enrolled in the study. Real Time PCR was performed to determine the expression of HERV-W-env and HERV-K-gag using GAPDH as housekeeping gene to infer relative quantification. Serum levels of cytokines IL-6, IL-10, and TNF-α were quantified using ELISA.

Results

HERV-W-env expression in CVD patients was 6-fold higher than the control group (p < 0.01), whereas HERV-K-gag was not expressed in CVD patients. CVD patients presented higher serum concentration of pro-inflammatory cytokines IL-6 and TNF-α than control group (p < 0.01). Positive correlation was observed between IL-10 and both TNF-α and IL-6 (p 〈0,01) and HERV-W and TNF-α/IL10 ratio in CVD patients.