脊髓损伤中miRNA-124-3p与SP1表达水平的相关性

IF 1 Q4 GENETICS & HEREDITY

Gene Reports Pub Date : 2025-04-23 DOI:10.1016/j.genrep.2025.102231

Maryam Zand , Mehdi Sadegh , Behzad Khansarinejad , Mahdieh Mondanizadeh

{"title":"脊髓损伤中miRNA-124-3p与SP1表达水平的相关性","authors":"Maryam Zand , Mehdi Sadegh , Behzad Khansarinejad , Mahdieh Mondanizadeh","doi":"10.1016/j.genrep.2025.102231","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><div>Spinal cord injury (SCI) is a serious traumatic event affecting the central nervous system, leading to severe mobility and functional impairments. MicroRNAs, particularly miR-124, play a crucial role in neurogenesis and neuronal protection in this condition. Concurrently, Specific Protein 1 (SP1) is involved in various cellular processes, including cell growth and apoptosis. This study investigates the relationship between miR-124-3p and SP1 expression levels in a rat model of spinal cord injury.</div></div><div><h3>Methods</h3><div>An experimental study was conducted using 72 male Wistar rats, divided into three groups: SCI, sham, and control. A standardized SCI model was established through a mechanical compression method at the T9-T10 spinal region. Motor function was assessed using the Basso, Beattie, and Bresnahan (BBB) scoring system at four-time points: 1 h, 1 day, 3 days, and 7 days post-injury. Histological examination was performed via Nissl staining, while RT-qPCR was used to quantify miR-124-3p and SP1 expression levels in spinal cord tissues.</div></div><div><h3>Results</h3><div>The BBB scores indicated a significant decline in motor function across all time points post-SCI. Histological analysis confirmed successful injury induction. Notably, miR-124-3p expression decreased significantly in the SCI group compared to controls, whereas SP1 levels increased significantly following injury.</div></div><div><h3>Conclusions</h3><div>This study demonstrates a marked reduction of miR-124-3p alongside an elevation of SP1 levels in response to SCI. These findings suggest a complex interplay between these molecules that may influence neuronal response and recovery mechanisms post-SCI. Understanding this relationship could provide insights into potential therapeutic targets for enhancing recovery following SCIs.</div></div>","PeriodicalId":12673,"journal":{"name":"Gene Reports","volume":"40 ","pages":"Article 102231"},"PeriodicalIF":1.0000,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Correlation of miRNA-124-3p and SP1 expression levels in spinal cord injury\",\"authors\":\"Maryam Zand , Mehdi Sadegh , Behzad Khansarinejad , Mahdieh Mondanizadeh\",\"doi\":\"10.1016/j.genrep.2025.102231\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><div>Spinal cord injury (SCI) is a serious traumatic event affecting the central nervous system, leading to severe mobility and functional impairments. MicroRNAs, particularly miR-124, play a crucial role in neurogenesis and neuronal protection in this condition. Concurrently, Specific Protein 1 (SP1) is involved in various cellular processes, including cell growth and apoptosis. This study investigates the relationship between miR-124-3p and SP1 expression levels in a rat model of spinal cord injury.</div></div><div><h3>Methods</h3><div>An experimental study was conducted using 72 male Wistar rats, divided into three groups: SCI, sham, and control. A standardized SCI model was established through a mechanical compression method at the T9-T10 spinal region. Motor function was assessed using the Basso, Beattie, and Bresnahan (BBB) scoring system at four-time points: 1 h, 1 day, 3 days, and 7 days post-injury. Histological examination was performed via Nissl staining, while RT-qPCR was used to quantify miR-124-3p and SP1 expression levels in spinal cord tissues.</div></div><div><h3>Results</h3><div>The BBB scores indicated a significant decline in motor function across all time points post-SCI. Histological analysis confirmed successful injury induction. Notably, miR-124-3p expression decreased significantly in the SCI group compared to controls, whereas SP1 levels increased significantly following injury.</div></div><div><h3>Conclusions</h3><div>This study demonstrates a marked reduction of miR-124-3p alongside an elevation of SP1 levels in response to SCI. These findings suggest a complex interplay between these molecules that may influence neuronal response and recovery mechanisms post-SCI. Understanding this relationship could provide insights into potential therapeutic targets for enhancing recovery following SCIs.</div></div>\",\"PeriodicalId\":12673,\"journal\":{\"name\":\"Gene Reports\",\"volume\":\"40 \",\"pages\":\"Article 102231\"},\"PeriodicalIF\":1.0000,\"publicationDate\":\"2025-04-23\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Gene Reports\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2452014425001049\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"GENETICS & HEREDITY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Gene Reports","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2452014425001049","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"GENETICS & HEREDITY","Score":null,"Total":0}

引用次数: 0

摘要

脊髓损伤（SCI）是一种影响中枢神经系统的严重创伤性事件，可导致严重的活动能力和功能障碍。在这种情况下，microrna，特别是miR-124，在神经发生和神经元保护中起着至关重要的作用。同时，特异性蛋白1 （SP1）参与多种细胞过程，包括细胞生长和凋亡。本研究探讨了miR-124-3p与SP1在脊髓损伤大鼠模型中的表达水平之间的关系。方法选用雄性Wistar大鼠72只，分为脊髓损伤组、假手术组和对照组。在T9-T10脊柱区采用机械压迫法建立标准化脊髓损伤模型。使用Basso， Beattie, and Bresnahan （BBB）评分系统在损伤后1小时、1天、3天和7天四个时间点评估运动功能。采用尼氏染色进行组织学检查，RT-qPCR定量检测miR-124-3p和SP1在脊髓组织中的表达水平。结果BBB评分显示脊髓损伤后各时间点运动功能显著下降。组织学分析证实损伤诱导成功。值得注意的是，与对照组相比，SCI组miR-124-3p的表达显著降低，而SP1水平在损伤后显著升高。结论：该研究表明，在脊髓损伤的反应中，miR-124-3p显著降低，SP1水平升高。这些发现表明，这些分子之间存在复杂的相互作用，可能影响脊髓损伤后神经元的反应和恢复机制。了解这种关系可以为增强SCIs后恢复的潜在治疗靶点提供见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Correlation of miRNA-124-3p and SP1 expression levels in spinal cord injury

Background

Spinal cord injury (SCI) is a serious traumatic event affecting the central nervous system, leading to severe mobility and functional impairments. MicroRNAs, particularly miR-124, play a crucial role in neurogenesis and neuronal protection in this condition. Concurrently, Specific Protein 1 (SP1) is involved in various cellular processes, including cell growth and apoptosis. This study investigates the relationship between miR-124-3p and SP1 expression levels in a rat model of spinal cord injury.

Methods

An experimental study was conducted using 72 male Wistar rats, divided into three groups: SCI, sham, and control. A standardized SCI model was established through a mechanical compression method at the T9-T10 spinal region. Motor function was assessed using the Basso, Beattie, and Bresnahan (BBB) scoring system at four-time points: 1 h, 1 day, 3 days, and 7 days post-injury. Histological examination was performed via Nissl staining, while RT-qPCR was used to quantify miR-124-3p and SP1 expression levels in spinal cord tissues.

Results

The BBB scores indicated a significant decline in motor function across all time points post-SCI. Histological analysis confirmed successful injury induction. Notably, miR-124-3p expression decreased significantly in the SCI group compared to controls, whereas SP1 levels increased significantly following injury.

Conclusions

This study demonstrates a marked reduction of miR-124-3p alongside an elevation of SP1 levels in response to SCI. These findings suggest a complex interplay between these molecules that may influence neuronal response and recovery mechanisms post-SCI. Understanding this relationship could provide insights into potential therapeutic targets for enhancing recovery following SCIs.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Gene Reports Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

3.30

自引率

7.70%

发文量

246

审稿时长

49 days

期刊介绍： Gene Reports publishes papers that focus on the regulation, expression, function and evolution of genes in all biological contexts, including all prokaryotic and eukaryotic organisms, as well as viruses. Gene Reports strives to be a very diverse journal and topics in all fields will be considered for publication. Although not limited to the following, some general topics include: DNA Organization, Replication & Evolution -Focus on genomic DNA (chromosomal organization, comparative genomics, DNA replication, DNA repair, mobile DNA, mitochondrial DNA, chloroplast DNA). Expression & Function - Focus on functional RNAs (microRNAs, tRNAs, rRNAs, mRNA splicing, alternative polyadenylation) Regulation - Focus on processes that mediate gene-read out (epigenetics, chromatin, histone code, transcription, translation, protein degradation). Cell Signaling - Focus on mechanisms that control information flow into the nucleus to control gene expression (kinase and phosphatase pathways controlled by extra-cellular ligands, Wnt, Notch, TGFbeta/BMPs, FGFs, IGFs etc.) Profiling of gene expression and genetic variation - Focus on high throughput approaches (e.g., DeepSeq, ChIP-Seq, Affymetrix microarrays, proteomics) that define gene regulatory circuitry, molecular pathways and protein/protein networks. Genetics - Focus on development in model organisms (e.g., mouse, frog, fruit fly, worm), human genetic variation, population genetics, as well as agricultural and veterinary genetics. Molecular Pathology & Regenerative Medicine - Focus on the deregulation of molecular processes in human diseases and mechanisms supporting regeneration of tissues through pluripotent or multipotent stem cells.