Erica Routila, Sadie Salminen, Randa Mahran, Mervi Toriseva, Heikki Irjala, Eeva Haapio, Eero Kytö, Sami Ventelä, Kim Pettersson, Johannes Routila, Kamlesh Gidwani, Janne Leivo
{"title":"整合素糖变异体作为头颈部鳞状细胞癌转移、侵袭和治疗分层的生物标志物的评估","authors":"Erica Routila, Sadie Salminen, Randa Mahran, Mervi Toriseva, Heikki Irjala, Eeva Haapio, Eero Kytö, Sami Ventelä, Kim Pettersson, Johannes Routila, Kamlesh Gidwani, Janne Leivo","doi":"10.1002/cam4.70717","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Background</h3>\n \n <p>Integrin glycosylation is one mechanism regulating the invasion and metastasis of malignant tumors. Little information exists about integrin glycosylation in head and neck squamous cell carcinoma (HNSCC). In this study, we evaluated the glycosylation of integrins in HNSCC tumor and serum samples.</p>\n </section>\n \n <section>\n \n <h3> Methods</h3>\n \n <p>Intraoperative fresh tumor and normal tissue samples and blood samples were collected from HNSCC patients (<i>N</i> = 24). Lectin-bioaffinity assays using six nanoparticle-bound lectins were used to evaluate the glycosylation of integrins ITGA2, ITGA3, ITGA5, ITGA6, ITGB1, and ITGB4. Associations with metastasis, therapy response, and clinical factors were analyzed.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>Glycosylation profiles of the integrins were relatively similar. High intratumoral ITGB1–WFL results were associated with high T class, whereas none of the integrin glycovariant assays provided significant resolution in the detection of nodal metastasis. While the serum integrin glycovariant levels were low overall, serum ITGA2–UEA offered significant resolution in both radiotherapy response prediction and cancer recurrence prognostication.</p>\n </section>\n \n <section>\n \n <h3> Conclusions</h3>\n \n <p>We demonstrate that while integrin glycovariants are abundant in HNSCC tumors and ITGB1-WFL was associated with invasiveness, integrin glycovariants do not directly correlate with metastatic behavior. Further, serum ITGA2–UEA appeared as a potential radioresponse biomarker.</p>\n </section>\n </div>","PeriodicalId":139,"journal":{"name":"Cancer Medicine","volume":"14 9","pages":""},"PeriodicalIF":2.9000,"publicationDate":"2025-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cam4.70717","citationCount":"0","resultStr":"{\"title\":\"Evaluation of Integrin Glycovariants as Biomarkers of Metastasis, Invasion, and Therapy Stratification in Head and Neck Squamous Cell Carcinoma\",\"authors\":\"Erica Routila, Sadie Salminen, Randa Mahran, Mervi Toriseva, Heikki Irjala, Eeva Haapio, Eero Kytö, Sami Ventelä, Kim Pettersson, Johannes Routila, Kamlesh Gidwani, Janne Leivo\",\"doi\":\"10.1002/cam4.70717\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n \\n <section>\\n \\n <h3> Background</h3>\\n \\n <p>Integrin glycosylation is one mechanism regulating the invasion and metastasis of malignant tumors. 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Evaluation of Integrin Glycovariants as Biomarkers of Metastasis, Invasion, and Therapy Stratification in Head and Neck Squamous Cell Carcinoma
Background
Integrin glycosylation is one mechanism regulating the invasion and metastasis of malignant tumors. Little information exists about integrin glycosylation in head and neck squamous cell carcinoma (HNSCC). In this study, we evaluated the glycosylation of integrins in HNSCC tumor and serum samples.
Methods
Intraoperative fresh tumor and normal tissue samples and blood samples were collected from HNSCC patients (N = 24). Lectin-bioaffinity assays using six nanoparticle-bound lectins were used to evaluate the glycosylation of integrins ITGA2, ITGA3, ITGA5, ITGA6, ITGB1, and ITGB4. Associations with metastasis, therapy response, and clinical factors were analyzed.
Results
Glycosylation profiles of the integrins were relatively similar. High intratumoral ITGB1–WFL results were associated with high T class, whereas none of the integrin glycovariant assays provided significant resolution in the detection of nodal metastasis. While the serum integrin glycovariant levels were low overall, serum ITGA2–UEA offered significant resolution in both radiotherapy response prediction and cancer recurrence prognostication.
Conclusions
We demonstrate that while integrin glycovariants are abundant in HNSCC tumors and ITGB1-WFL was associated with invasiveness, integrin glycovariants do not directly correlate with metastatic behavior. Further, serum ITGA2–UEA appeared as a potential radioresponse biomarker.
期刊介绍：
Cancer Medicine is a peer-reviewed, open access, interdisciplinary journal providing rapid publication of research from global biomedical researchers across the cancer sciences. The journal will consider submissions from all oncologic specialties, including, but not limited to, the following areas:
Clinical Cancer Research
Translational research ∙ clinical trials ∙ chemotherapy ∙ radiation therapy ∙ surgical therapy ∙ clinical observations ∙ clinical guidelines ∙ genetic consultation ∙ ethical considerations
Cancer Biology:
Molecular biology ∙ cellular biology ∙ molecular genetics ∙ genomics ∙ immunology ∙ epigenetics ∙ metabolic studies ∙ proteomics ∙ cytopathology ∙ carcinogenesis ∙ drug discovery and delivery.
Cancer Prevention:
Behavioral science ∙ psychosocial studies ∙ screening ∙ nutrition ∙ epidemiology and prevention ∙ community outreach.
Bioinformatics:
Gene expressions profiles ∙ gene regulation networks ∙ genome bioinformatics ∙ pathwayanalysis ∙ prognostic biomarkers.
Cancer Medicine publishes original research articles, systematic reviews, meta-analyses, and research methods papers, along with invited editorials and commentaries. Original research papers must report well-conducted research with conclusions supported by the data presented in the paper.
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